In:
American Journal of Gastroenterology, 2010, Vol.105(9), pp.2060-2071
Description:
OBJECTIVES:: METHODS:: RESULTS:: Expression profiling showed 272 upregulated genes, including those encoding for immunoglobulins, chemokines and their receptors, and 86 downregulated genes, including those for pancreatic proteases such as three trypsinogen isoforms. Protein profiling showed that the expression of trypsinogens and other pancreatic enzymes was greatly reduced. Immunohistochemistry showed a near-loss of trypsin-positive acinar cells, which was also confirmed by western blotting. The serum of AIP patients contained high titers of autoantibodies against the trypsinogens PRSS1 and PRSS2 but not against PRSS3. In addition, there were autoantibodies against the trypsin inhibitor PSTI (the product of the SPINK1 gene). In the pancreas of AIP animals, we found similar protein patterns and a reduction in trypsinogen. CONCLUSIONS::
Keywords:
Medicine;
ISSN:
0002-9270
E-ISSN:
15720241
DOI:
10.1038/ajg.2010.141
URL:
View record at Wolters Kluwer Health Ovid Technologies
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