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  • 1
    In: Environmental Microbiology, June 2016, Vol.18(6), pp.1988-2000
    Description: Phosphorus () is an important macronutrient for all biota on earth but similarly a finite resource. Microorganisms play on both sides of the fence as they effectively mineralize organic and solubilize precipitated forms of soil phosphorus but conversely also take up and immobilize . Therefore, we analysed the role of microbes in two beech forest soils with high and low content by direct sequencing of metagenomic deoxyribonucleic acid. For inorganic solubilization, a significantly higher microbial potential was detected in the ‐rich soil. This trait especially referred to  olibacter usiatus, likewise one of the dominating species in the data sets. A higher microbial potential for efficient phosphate uptake systems () was detected in the ‐depleted soil. Genes involved in starvation response regulation (, ) were prevalent in both soils. This underlines the importance of effective phosphate (ho) regulon control for microorganisms to use alternative sources during phosphate limitation. Predicted genes were primarily harboured by hizobiales, ctinomycetales and cidobacteriales.
    Keywords: Soil Microbiology – Analysis ; Nucleic Acids – Analysis ; Phosphates – Analysis ; Forest Soils – Analysis ; Soil Phosphorus – Analysis;
    ISSN: 1462-2912
    E-ISSN: 1462-2920
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  • 2
    Language: English
    In: Journal of Plant Nutrition and Soil Science, August 2016, Vol.179(4), pp.472-480
    Description: Among several environmental factors shaping soil microbial communities the impact of soil nutrients is of special interest. While continuous application mainly of N and P dramatically shifts community composition during fertilization, it remains unclear whether this effect is consistent in generic, unfertilized beech forest ecosystems of Germany, where differences in nutrient contents are mostly a result of the parental material and climatic conditions. We postulate that in such ecosystems nutrient effects are less pronounced due to the possibility of the soil microbiome to adapt to the corresponding conditions over decades and the vegetation acts as the major driver. To test this hypothesis, we investigated the bacterial community composition in five different German beech dominated forest soils, representing a natural gradient of total‐ and easily available mineral‐P. A community fingerprinting approach was performed using terminal‐Restriction Fragment Length Polymorphism analysis of the 16S rRNA gene, while abundance of bacteria was measured applying quantitative real‐time PCR. Bacterial communities at the five forest sites were distinctly separated, with strongest differences between the end‐members of the P‐gradient. However the majority of identified microbial groups (43%) were present at all sites, forming a core microbiome independent from the differences in soil chemical properties. Especially in the P‐deficient soil the abundance of unique bacterial groups was highly increased, indicating a special adaption of the community to P limitation at this site. In this regard Correspondence Analysis elucidated that exclusively soil pH significantly affected community composition at the investigated sites. In contrast soil C, N and P contents did mainly affect the overall abundance of bacteria.
    Keywords: Core Microbiome ; Forest Soil ; Nutrient Content ; Diversity
    ISSN: 1436-8730
    E-ISSN: 1522-2624
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  • 3
    In: Environmental Microbiology, September 2016, Vol.18(8), pp.2767-2767
    Description: Byline: Fabian Bergkemper, Anne Scholer, Marion Engel, Friederike Lang, Jaane Kruger, Michael Schloter, Stefanie Schulz ***** No abstract is available for this article. *****
    Keywords: Recycling ; Forest Soils ; Soil Microbiology;
    ISSN: 1462-2912
    E-ISSN: 1462-2920
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  • 4
    Language: English
    In: Journal of Microbiological Methods, June 2016, Vol.125, pp.91-97
    Description: Phosphorus (P) is of central importance for cellular life but likewise a limiting macronutrient in numerous environments. Certainly microorganisms have proven their ability to increase the phosphorus bioavailability by mineralization of organic-P and solubilization of inorganic-P. On the other hand they efficiently take up P and compete with other biota for phosphorus. However the actual microbial community that is associated to the turnover of this crucial macronutrient in different ecosystems remains largely anonymous especially taking effects of seasonality and spatial heterogeneity into account. In this study seven oligonucleotide primers are presented which target genes coding for microbial acid and alkaline phosphatases ( , ), phytases ( ), phosphonatases ( ) as well as the quinoprotein glucose dehydrogenase ( ) and different P transporters ( , ). Illumina amplicon sequencing of soil genomic DNA underlined the high rate of primer specificity towards the respective target gene which usually ranged between 98% and 100% ( : 87%). As expected the primers amplified genes from a broad diversity of distinct microorganisms. Using DNA from a beech dominated forest soil, the highest microbial diversity was detected for the alkaline phosphatase ( ) gene which was amplified from 15 distinct phyla respectively 81 families. Noteworthy the primers also allowed amplification of from 6 fungal orders. The genes coding for acid phosphatase ( ) and the quinoprotein glucose dehydrogenase ( ) were amplified from 20 respectively 17 different microbial orders. In comparison the phytase and phosphonatase ( , ) primers covered 13 bacterial orders from 2 different phyla respectively. Although the amplified microbial diversity was apparently limited both primers reliably detected all orders that contributed to the P turnover in the investigated soil as revealed by a previous metagenomic approach. Genes that code for microbial P transporter ( , ) were amplified from 13 respectively 9 distinct microbial orders. Accordingly the introduced primers represent a valuable tool for further analysis of the microbial community involved in the turnover of phosphorus in soils but most likely also in other environments.
    Keywords: Phosphorus Turnover ; Forest Soil ; Phod ; Phon ; Appa ; Pita ; Psts ; Biology
    ISSN: 0167-7012
    E-ISSN: 1872-8359
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