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  • 1
    In: The Journal of Cell Biology, Feb 7, 2000, Vol.148(3), p.567
    Description: The anterior-posterior axis of the mouse embryo is defined before formation of the primitive streak, and axis specification and subsequent anterior development involves signaling from both embryonic ectoderm and visceral endoderm. The Wnt signaling pathway is essential for various developmental processes, but a role in anterior-posterior axis formation in the mouse has not been previously established. [Beta]-Catenin is a central player in the Wnt pathway and in cadherin-mediated cell adhesion. We generated [Beta]-catenin-deficient mouse embryos and observed a defect in anterior-posterior axis formation at embryonic day 5.5, as visualized by the absence of Hex and Hesx1 and the mislocation of cerberus-like and Lim1 expression. Subsequently, no mesoderm and head structures are generated. Intercellular adhesion is maintained since plakoglobin substitutes for [Beta]-catenin. Our data demonstrate that [Beta]-catenin function is essential in anterior-posterior axis formation in the mouse, and experiments with chimeric embryos show that this function is required in the embryonic ectoderm. Key words: anterior visceral endoderm * Wnt/wingless pathway * cell adhesion * plakoglobin * armadillo
    Keywords: Cell Adhesion -- Research ; Armadillos -- Research
    ISSN: 0021-9525
    E-ISSN: 15408140
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  • 2
    In: Biology of Reproduction, 2000, Vol.63(5), p.1555-1561
    Description: Abstract We developed a novel promoter-based selection strategy that could be used to produce cell lines representing sequential stages of spermatogenesis. The method is based on immortalization and subsequent targeted selection by using differentiation-specific promoter regions. As an example for this approach, a new murine germ cell line (GC-4spc) was established using a vector construct that contains the SV40 large T antigen and the neomycin phosphotransferase II gene under the control of the SV40 early promoter and a spermatocyte-specific promoter for human phosphoglycerate kinase 2, respectively. The GC-4spc was characterized as a cell line at the stage between preleptotene and early pachytene spermatocytes. Transcription of three germ cell-specific expressed genes, Pgk2, proacrosin, and the A-myb proto-oncogene, were detected in the GC-4spc cell line using reverse transcription-polymerase chain reaction. Furthermore, TSPY (human testis-specific protein, Y-encoded) and PGK2 (human phosphoglycerate kinase 2) promoter regions showed different transcriptional activities in the GC-4spc cell line compared with the spermatogonia-derived cell line GC-1spg. Thus, our strategy could be used for immortalization of cells at specific stages of differentiation, allowing production of a series of cultured cell lines representing sequential stages of differentiation in given cell lineages.
    Keywords: Spermatogenesis ; Testes
    ISSN: 0006-3363
    E-ISSN: 15297268
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  • 3
    In: Angewandte Chemie International Edition, 4/17/2000, Vol.39(8), pp.1453-1455
    ISSN: 14337851
    E-ISSN: 15213773
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