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  • 1
    Language: English
    In: The Lancet Global Health, July 2014, Vol.2(7), pp.e387-e387
    Keywords: Public Health
    ISSN: 2214-109X
    E-ISSN: 2214-109X
    Source: ScienceDirect Journals (Elsevier)
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  • 2
    Language: English
    In: The Journal of Pediatrics, 1986, Vol.108(2), pp.247-249
    Keywords: Medicine
    ISSN: 0022-3476
    E-ISSN: 1097-6833
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  • 3
    Language: English
    In: Microbes and Infection, 2006, Vol.8(9), pp.2465-2468
    Description: causes the sexually transmitted genital ulcer disease chancroid. In human inoculation experiments, bacteria colocalize with neutrophils and macrophages but remain extracellular. The organism also colocalizes with collagen and fibrin but not with keratinocytes, fibroblasts, laminin, or fibronectin. These relationships are established by 48 h postinoculation and persist through the pustular stage of disease. To extend these observations to the ulcerative stage of disease, and to compare results in the human model with those of natural disease, we obtained biopsies from patients with naturally acquired chancroid. All ulcers were culture positive for and histologically very similar to pustules from the human model. Staining with -specific monoclonal antibodies demonstrated within 5 biopsies. The organism was chiefly found within the granulocytic infiltrate of the ulcer. Dual staining for and eukaryotic tissue components showed that colocalized with neutrophils and fibrin at the ulcerative stage of disease. No bacteria were associated with keratinocytes, fibroblasts, or collagen. Overall, these findings are consistent with results from the human model. This is the first reported study to localize bacteria specifically identified as within naturally acquired chancroid.
    Keywords: Haemophilus Ducreyi ; Chancroid ; Genital Ulcer ; Bacterial Localization ; Biology
    ISSN: 1286-4579
    E-ISSN: 1769-714X
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  • 4
    Language: English
    In: The Journal of Pediatrics, 1986, Vol.108(2), pp.169-177
    Description: To document pulmonary function abnormalities in children with sarcoidosis, we reviewed the records of all children with this disease at the North Carolina Memorial Hospital. Spirometry was performed by 34 of 60 children at initial presentation and was repeated by 16 at least 1 yr after presentation (mean, 3.9 yr). Those with and without pulmonary function data available were similar in age, sex, race, and clinical manifestations at presentation. Results indicate that at presentation, 50% of children had the characteristic functional changes of restrictive lung disease (mean % predicted FVC, 79.8 +/- 16.0). Fifteen percent had a FVC of 70 to 80% predicted, 32% had a FVC of 50 to 70% predicted, and 3% had a FVC less than 50% predicted. The TLC and FRC supported the diagnosis of restrictive lung disease. There were 15% who had obstructive changes. Children with auscultatory abnormalities and parenchymal changes on chest radiograph had more severe decrements in pulmonary function; however, specific symptoms did not predict decrements in pulmonary function. There was significant improvement in lung function at the last pulmonary function test performed (mean % predicted FVC, 97.1 +/- 18.8).
    Keywords: Medicine
    ISSN: 0022-3476
    E-ISSN: 1097-6833
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  • 5
    Language: English
    In: The Journal of Pediatrics, 1986, Vol.109(5), pp.835-838
    Keywords: Medicine
    ISSN: 0022-3476
    E-ISSN: 1097-6833
    Source: ScienceDirect Journals (Elsevier)
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  • 6
    Language: English
    In: The Journal of Pediatrics, 1981, Vol.98(2), pp.328-332
    Keywords: Medicine
    ISSN: 0022-3476
    E-ISSN: 1097-6833
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  • 7
    Language: English
    In: Microbial Pathogenesis, 2006, Vol.40(3), pp.110-115
    Description: express two porin proteins, termed OmpP2A and OmpP2B. To test whether expression of OmpP2A and OmpP2B was necessary for virulence in humans, eight volunteers were experimentally infected with the parent (35000HP) in one arm and a double OmpP2A OmpP2B mutant (35000HP::P2AB) in the other arm. The pustule formation rates were 58.3% (95% CI, 33.2–83.5%) for the parent and 41.7% (95% CI, 19.3–64.0%) for the mutant ( =0.25). Biopsy of 35000HP and 35000HP::P2AB-infected sites yielded similar amounts of bacteria in quantitative culture. These results indicate that expression of OmpP2A and OmpP2B is not necessary to initiate disease or to progress to pustule formation in humans.
    Keywords: Pustule Formation ; Haemophilus Ducreyi ; Porin Proteins ; Uirulence ; Human Challenge Model ; Biology ; Chemistry
    ISSN: 0882-4010
    E-ISSN: 1096-1208
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  • 8
    Language: English
    In: Journal of Immunological Methods, 1985, Vol.81(1), pp.161-165
    Description: We compared bovine serum albumin, commercial non-fat dry milk, and Tween 20 as blocking agents for immunologic probing of bacterial proteins transferred to nitrocellulose sheets. There were quantitative and qualitative differences in antigens detected that depended on which blocking agents were used. We suggest that several methods for blocking and washing nitrocellulose should be compared when Western blotting is used to detect immunologically reactive proteins.
    Keywords: Western Blotting ; Blocking Agents ; Medicine ; Biology
    ISSN: 0022-1759
    E-ISSN: 1872-7905
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  • 9
    Language: English
    In: Microbial Pathogenesis, 1994, Vol.16(3), pp.243-247
    Description: Haemophilus ducreyi, Moraxella catarrhalis and a non-piliated Escherichia coli K-12 strain were studied for their ability to bind to human keratinocytes in vitro. Epidermal cells isolated from neonatal foreskins were grown to confluence in serum-free keratinocyte media. Probing of the monolayers with anti-cytokeratin antibody showed that 97% of cells were keratinocytes. Bacteria were grown to mid-log phase and seeded onto the monolayers. At various time-points monolayers were washed with PBS to remove non-adherent bacteria, and the monolayers were quantitatively cultured. After 120 min, 15 to 23% of the H. ducreyi inocula bound to the monolayer, while less than 1% of the M. catarrhalis or E. coli controls bound. Wet mounts of fixed monolayers observed with differential interference contrast microscopy confirmed the quantitative data. We conclude that H. ducreyi binds to keratinocytes and that this process may play a role in the initiation of chancroid.
    Keywords: Haemophilus Ducreyi; Adherence; Keratinocytes ; Biology ; Chemistry
    ISSN: 0882-4010
    E-ISSN: 1096-1208
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  • 10
    Language: English
    In: Microbial Pathogenesis, 1990, Vol.9(6), pp.417-426
    Description: Twelve strains of Haemophilus ducreyi isolated primarily from chancroid outbreaks in North America were examined for the presence of pili by transmission electron microscopy. We identified piliated cells in 10 of the 12 strains. Pilin extracts were prepared from the mechanically sheared cells of the 12 H. ducreyi strains as well as the stably piliated H. influenzae strain R890 and its non-piliated parent R906. Pili were present in 12 out of 12 H. ducreyi extracts and in the R890 extract but not in the R906 preparation. Pili were purified by cycles of differential pH solubilization and crystallization. In SDS-PAGE, the preparation consisted predominantly of a protein whose apparent relative molecular mass was 24,000 (24 k), and an electron micrograph showed that the preparation contained pili. Three H. ducreyi strains were passed 52 times on agar plates, and extracts prepared from these strains contained pili. There was no evidence of binding of erythrocytes obtained from nine mammalian and avian species to colonies of one of the stably piliated H. ducreyi strains. We conclude that H. ducreyi expressed pili, that the relative molecular mass of the pilin monomer was 24 k, that pilus expression was not readily lost in passage and that H. ducreyi pili may not bind to an erythrocyte receptor.
    Keywords: Haemophilus Ducreyi ; Pili ; Fimbriae ; Biology ; Chemistry
    ISSN: 0882-4010
    E-ISSN: 1096-1208
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