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  • HAL (CCSd)  (32)
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  • 1
    Language: English
    In: Études de communication, 01 June 2013, pp.145-166
    Description: Cet article vise à spécifier comment la quantification d’un phénomène fondée sur une certaine vision du monde se propage et se déforme. Ce projet a été porté par une direction déconcentrée de l’État soucieuse de l’emprise croissante du bâti sur les terres agricoles en Languedoc-Roussillon. Le cadre théorique mobilisé, la sociologie de la traduction, permet d’identifier les grandes étapes d’intéressement, d’enrôlement, ainsi que le développement de controverses. Les difficultés rencontrées interrogent sur la complexité de clôture de processus innovants s’agissant de production d’information et de co-construction de sens associant des actants hétérogènes impliqués dans des procédures de débat public.
    Keywords: Sociologie de La Traduction ; Qualité Des Terres Agricoles ; Dissidence ; Production D’information ; Production de Sens ; Actor-Network Theory ; Agricultural Soil Quality ; Dissidence ; Information Production ; Sensemaking ; Journalism & Communications
    ISSN: 1270-6841
    E-ISSN: 2101-0366
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  • 2
    Language: French
    Description: The land-use planning in France, and specifically the making process of planning documents, evolve both in a distinct and common way due to the effects of the rationalization of public policies, the State devolution of power and the transformations of land management issues. The actors...
    Keywords: Humanities and Social Sciences ; Library and Information Sciences ; Constitutive Communication ; Interorganizational Collaboration ; Authoring Process ; Prescription ; Big Data ; Rawification ; Instrumentation ; Sensemaking ; Soils Artificialization ; Land Use Planning ; Communication Constitutive ; Collaboration Inter-Organisationnelle ; Processus D'Auteurisation ; Brutification ; Travail Du Sens ; Artificialisation Des Sols ; Aménagement Du Territoire
    Source: Hyper Article en Ligne (CCSd)
    Source: Hyper Article en Ligne Open Access (CCSd)
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  • 3
    Language: French
    Description: The land-use planning in France, and specifically the making process of planning documents, evolve both in a distinct and common way due to the effects of the rationalization of public policies, the State devolution of power and the transformations of land management issues. The actors...
    Keywords: Computer Science ; Information Theory ; Rawification ; Authoring Process ; Interorganizational Collaboration ; Constitutive Communication ; Instrumentation ; Sensemaking ; Soils Artificialization ; Land Use Planning ; Communication Constitutive ; Collaboration Inter-Organisationnelle ; Processus D'Auteurisation ; Prescription ; Big Data ; Brutification ; Travail Du Sens ; Artificialisation Des Sols ; Aménagement Du Territoire
    Source: Hyper Article en Ligne (CCSd)
    Source: Hyper Article en Ligne Open Access (CCSd)
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  • 4
    Language: French
    In: Études de communication - Langages, information, médiations, 2013, Issue 40, pp.145-166
    Description: This article aims to describe how the quantification of a phenomenon in relation to a specific worldview spreads and is distorted. This project has been supported by a regional public authority in Languedoc-Roussillon (France), and consisted...
    Keywords: Environmental Sciences ; Agricultural Land ; Geographical Information Systems ; Geomatics ; Farmland ; Territorial Community ; Systeme D'Information Geographique ; Geomatique ; Terre Agricole ; Bati ; Collecte D'Information ; Collectivite Territoriale ; Environmental Sciences ; Journalism & Communications
    ISSN: 1270-6841
    E-ISSN: 2101-0366
    Source: Hyper Article en Ligne (CCSd)
    Source: Hyper Article en Ligne Open Access (CCSd)
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  • 5
    Language: English
    In: Acta neurochirurgica, June 2013, Vol.155(6), pp.1095-100; discussion 1100
    Description: International guidelines for the management of unruptured intracranial aneurysms (UIAs) recommend observation in aneurysms 〈10 mm due to the estimated low risk of rupture. The aim of our study was analyse the data of recently treated patients with ruptured cerebral aneurysms with the special focus on size and configuration in view of the frequency scale in a daily routine setting. We reviewed the data of all patients with aneurysmal subarachnoid haemorrhage (SAH) during the last 24 months at our institution. Configuration and size of the aneurysms were measured. Clinical data were collected using the following classifications for analysis: Hunt and Hess (H&H), modified Rankin Scale (mRS) and Fisher classification. Data of 135 patients with aneurysmal SAH (98 women, 37 men; ratio 2.6:1) were analysed. Analysis showed that 19 aneurysms (14 %) were 〉10 mm (mean size, 19.2 mm) and 116 aneurysms (85.9 %) 10 mm), 18 as multi-lobar (n = 16 10 mm) and 5 as fusiform (n = 4 10 mm). Since the results of our study showed that the majority of the aneurysms are 〈10 mm (mean, 6.2 mm), it is justified to challenge the recommendations of the international guidelines in a daily routine setting. We believe that the published data are not convincing enough to play a guidance role in daily routine. Due to improving surgical and endovascular techniques with satisfying results and the high number of ruptured small aneurysms, we believe a change in attitude in management of small-sized aneurysms is needed. Further diagnostic models are needed to determine the risk of rupture of intracranial aneurysms properly to obtain adequate treatment for UIAs.
    Keywords: Aneurysm, Ruptured -- Surgery ; Intracranial Aneurysm -- Surgery ; Practice Guidelines As Topic -- Standards ; Subarachnoid Hemorrhage -- Surgery
    ISSN: 00016268
    E-ISSN: 0942-0940
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  • 6
    Language: English
    In: Nature Communications, 14 June 2016, Vol.7, p.11847
    Description: Calcium (Ca 2+ ) and redox signalling play important roles in acclimation processes from archaea to eukaryotic organisms. Herein we characterized a unique protein from Chlamydomonas reinhardtii that has the competence to integrate Ca 2+ - and redox-related signalling. This protein, designated as calredoxin (CRX), combines four Ca 2+ -binding EF-hands and a thioredoxin (TRX) domain. A crystal structure of CRX, at 1.6 Å resolution, revealed an unusual calmodulin-fold of the Ca 2+ -binding EF-hands, which is functionally linked via an inter-domain communication path with the enzymatically active TRX domain. CRX is chloroplast-localized and interacted with a chloroplast 2-Cys peroxiredoxin (PRX1). Ca 2+ -binding to CRX is critical for its TRX activity and for efficient binding and reduction of PRX1. Thereby, CRX represents a new class of Ca 2+ -dependent ‘sensor-responder' proteins. Genetically engineered Chlamydomonas strains with strongly diminished amounts of CRX revealed altered photosynthetic electron transfer and were affected in oxidative stress response underpinning a function of CRX in stress acclimation. Calcium and redox signalling have important roles in acclimation processes. Here, the authors characterise a protein from Chlamydomonas reinhardtii that can integrate calcium and redox-related signalling.
    Keywords: Life Sciences ; Life Sciences ; Biochemistry, Molecular Biology ; Algae ; Chlamydomonas Reinhardtii ; Calcium ; Redox Signaling ; Protein ; Calredoxin ; Crx ; Subcellular Localization ; Chloroplast ; Crystal Structure ; Thioredoxin Domain ; Photosynthesis ; Electron Transfer ; Oxidative Stress ; Stress Acclimation ; Biology
    ISSN: 2041-1723
    E-ISSN: 2041-1723
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  • 7
    Language: English
    In: Molecular & cellular proteomics : MCP, November 2013, Vol.12(11), pp.3160-83
    Description: Chlamydomonas reinhardtii is a green unicellular eukaryotic model organism for studying relevant biological and biotechnological questions. The availability of genomic resources and the growing interest in C. reinhardtii as an emerging cell factory for the industrial production of biopharmaceuticals require an in-depth analysis of protein N-glycosylation in this organism. Accordingly, we used a comprehensive approach including genomic, glycomic, and glycoproteomic techniques to unravel the N-glycosylation pathway of C. reinhardtii. Using mass-spectrometry-based approaches, we found that both endogenous soluble and membrane-bound proteins carry predominantly oligomannosides ranging from Man-2 to Man-5. In addition, minor complex N-linked glycans were identified as being composed of partially 6-O-methylated Man-3 to Man-5 carrying one or two xylose residues. These findings were supported by results from a glycoproteomic approach that led to the identification of 86 glycoproteins. Here, a combination of in-source collision-induced dissodiation (CID) for glycan fragmentation followed by mass tag-triggered CID for peptide sequencing and PNGase F treatment of glycopeptides in the presence of (18)O-labeled water in conjunction with CID mass spectrometric analyses were employed. In conclusion, our data support the notion that the biosynthesis and maturation of N-linked glycans in the endoplasmic reticulum and Golgi apparatus occur via a GnT I-independent pathway yielding novel complex N-linked glycans that maturate differently from their counterparts in land plants.
    Keywords: Algal Proteins -- Chemistry ; Chlamydomonas Reinhardtii -- Metabolism ; Glycoproteins -- Chemistry
    ISSN: 15359476
    E-ISSN: 1535-9484
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  • 8
    Language: English
    In: PLoS ONE, 2012, Vol.7(2), p.e30624
    Description: Whole genome sequencing allowed the development of a number of high resolution sequence based typing tools for Yersinia (Y.) pestis . The application of these methods on isolates from most known foci worldwide and in particular from China and the Former Soviet Union has dramatically improved our understanding of the population structure of this species. In the current view, Y. pestis including the non or moderate human pathogen Y. pestis subspecies microtus emerged from Yersinia pseudotuberculosis about 2,600 to 28,600 years ago in central Asia. The majority of central Asia natural foci have been investigated. However these investigations included only few strains from Mongolia. ; Clustered Regularly Interspaced Short Prokaryotic Repeats (CRISPR) analysis and Multiple-locus variable number of tandem repeats (VNTR) analysis (MLVA) with 25 loci was performed on 100 strains, isolated from 37 sampling areas in Mongolia. The resulting data were compared with previously published data from more than 500 plague strains, 130 of which had also been previously genotyped by single nucleotide polymorphism (SNP) analysis. The comparison revealed six main clusters including the three biovars Ulegeica, Altaica, and Xilingolensis. The largest cluster comprises 78 isolates, with unique and new genotypes seen so far in Mongolia only. Typing of selected isolates by key SNPs was used to robustly assign the corresponding clusters to previously defined SNP branches. ; We show that Mongolia hosts the most recent clade (Ulegeica). Interestingly no representatives of the ancestral subspecies nodes previously identified in North-western China were identified in this study. This observation suggests that the subsequent evolution steps within did not occur in Mongolia. Rather, Mongolia was most likely re-colonized by more recent clades coming back from China contemporary of the black death pandemic, or more recently in the past 600 years.
    Keywords: Research Article ; Biology ; Computational Biology ; Evolutionary Biology ; Microbiology ; Medicine ; Infectious Diseases
    E-ISSN: 1932-6203
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  • 9
    Language: English
    In: Biotechnology Letters, 2011, Vol.33(3), pp.495-502
    Description: A new method for sampling suspended animal cells by fast filtration is presented that allows rapid quenching of cellular metabolism and efficient separation of the cells from culture medium. Compared to sampling with a microstructure heat exchanger or centrifugation without prior quenching, the adenylate energy charge and the measured concentrations especially of metabolites with a high turnover rate or of metabolites early in metabolic pathways were substantially higher. No leakage of ATP from the cells was observed when using iso-osmotic NaCl solution in the washing step. The combination of fast filtration and cold methanol extraction is therefore suitable for intracellular metabolomic studies of suspended animal cell cultures and superior to other methods currently applied.
    Keywords: Animal cells ; Fast filtration ; Metabolome ; Microstructure heat exchanger ; Quenching ; Rapid sampling
    ISSN: 0141-5492
    E-ISSN: 1573-6776
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  • 10
    Language: English
    In: PLANT 2030 Status Seminar 2012, Program KBBE
    Keywords: Life Sciences ; Vegetal Biology ; Life Sciences ; Biotechnology ; Life Sciences ; Biochemistry, Molecular Biology ; Genomics ; Life Sciences ; Vegetal Biology ; Phytopathology and Phytopharmacy ; Life Sciences ; Vegetal Biology ; Plant Breeding ; Life Sciences ; Biochemistry, Molecular Biology ; Molecular Biology ; Life Sciences ; Cellular Biology ; Life Sciences ; Biochemistry, Molecular Biology
    Source: Hyper Article en Ligne (CCSd)
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