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Berlin Brandenburg

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  • Health Reference Center Academic (Gale)  (9)
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  • 1
    In: Canadian Journal of Microbiology (Revue canadienne de microbiologie), 2006, Vol.52(8), pp.731-739
    Description: A real-time polymerase chain reaction (PCR) method was applied to quantify the nifH gene pool in cucumber shoot and root and to evaluate how nitrogen (N) supply and plant age affect the nifH gene pool. In shoots, the relative abundance of the nifH gene was affected neither by different stages of plant growth nor by N supply. In roots, higher numbers of diazotrophic bacteria were found compared with that in the shoot. The nifH gene pool in roots significantly increased with plant age, and unexpectedly, the pool size was positively correlated with N supply. The relative abundance of nifH gene copy numbers in roots was also positively correlated ( r =0.96) with total N uptake of the plant. The data suggest that real-time PCR-based nifH gene quantification in combination with N-content analysis can be used as an efficient way to perform further studies to evaluate the direct contribution of the N 2 -fixing plant-colonizing plant growth promoting bacteria to plant N nutrition. Key words: real-time PCR, biological nitrogen fixation, cucumber, N nutrition, plant growth promoting bacteria.
    Description: Nous avons employ une mthode de raction en chane de la polymrase (PCR) en temps rel afin de quantifier l'ensemble des gnes nifH dans les pousses et les racines de concombres et d'valuer comment l'apport de N et l'ge de la plante influencent l'ensemble des gnes nifH . Dans les pousses, l'abondance relative du gne nifH n'a t affecte ni par les diffrents stades de croissance vgtale ni par l'apport en N. Dans les racines, un nombre suprieur de bactries diazotrophes a t retrouv comparativement aux pousses. L'ensemble des gnes nifH dans les racines a augment significativement avec l'ge de la plante et la taille de l'ensemble gntique fut inopinment corrle positivement avec l'apport en N. L'abondance relative des nombres de copies du gne nifH dans les racines fut galement corrle positivement ( r =0,96) avec l'apport total de N de la plante. Les donnes indiquent que la quantification du gne nifH par PCR en temps rel combine avec l'analyse du contenu en N peut tre employe comme mthode efficace pour raliser davantage d'tudes afin d'valuer la contribution directe des bactries fixatrices de N 2 colonisant les plantes et favorisant la croissance vgtale sur l'alimentation de la plante en N. Mots cls : PCR en temps rel, fixation biologique de l'azote, concombre, alimentation en N, bactrie favorisant la croissance vgtale.[Traduit par la Rdaction]
    Keywords: ProtÉ ; Ases;
    ISSN: 0008-4166
    E-ISSN: 1480-3275
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  • 2
    Language: English
    In: Science, 30 June 2006, Vol.312(5782), pp.1937-1941
    Description: The hexavalent state, considered to be the highest oxidation level accessible for iron, has previously been found only in the tetrahedral ferrate dianion, $FeO_4^{2-}$ . We report the photochemical synthesis of another Fe(VI) compound, an octahedrally coordinated dication bearing a terminal nitrido ligand. $M\ddot{o}ssbauer$ and x-ray absorption spectra, supported by density functional theory, are consistent with the octahedral structure having an $Fe \equiv N$ triple bond of 1.57 angstroms and a singlet $d_{xy}^2$ ground electronic configuration. The compound is stable at 77 kelvin and yields a highspin Fe(lll) species upon warming.
    Keywords: Physical sciences -- Chemistry -- Chemical reactions ; Physical sciences -- Physics -- Microphysics ; Physical sciences -- Chemistry -- Stereochemistry ; Physical sciences -- Physics -- Microphysics ; Physical sciences -- Chemistry -- Chemical compounds ; Physical sciences -- Chemistry -- Chemical bonding ; Physical sciences -- Physics -- Microphysics ; Physical sciences -- Chemistry -- Chemical elements ; Biological sciences -- Biology -- Biological taxonomies ; Physical sciences -- Physics -- Microphysics
    ISSN: 00368075
    E-ISSN: 10959203
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  • 3
    Language: English
    In: Circulation, Nov 10, 2015, Vol.132(19)
    Keywords: Heart Cells – Research ; Exome Sequencing – Research ; Electrophysiology – Usage ; Long Qt Syndrome – Care and Treatment
    ISSN: 0009-7322
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  • 4
    Language: English
    In: The Journal of clinical investigation, 01 March 2018, Vol.128(3), pp.1043-1056
    Description: Congenital long QT syndrome (LQTS) is an inherited channelopathy associated with life-threatening arrhythmias. LQTS type 2 (LQT2) is caused by mutations in KCNH2, which encodes the potassium channel hERG. We hypothesized that modifier genes are partly responsible for the variable phenotype severity observed in some LQT2 families. Here, we identified contributors to variable expressivity in an LQT2 family by using induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) and whole exome sequencing in a synergistic manner. We found that iPSC-CMs recapitulated the clinical genotype-phenotype discordance in vitro. Importantly, iPSC-CMs derived from the severely affected LQT2 patients displayed prolonged action potentials compared with cells from mildly affected first-degree relatives. The iPSC-CMs derived from all patients with hERG R752W mutation displayed lower IKr amplitude. Interestingly, iPSC-CMs from severely affected mutation-positive individuals exhibited greater L-type Ca2+ current. Whole exome sequencing identified variants of KCNK17 and the GTP-binding protein REM2, providing biologically plausible explanations for this variable expressivity. Genome editing to correct a REM2 variant reversed the enhanced L-type Ca2+ current and prolonged action potential observed in iPSC-CMs from severely affected individuals. Thus, our findings showcase the power of combining complementary physiological and genomic analyses to identify genetic modifiers and potential therapeutic targets of a monogenic disorder. Furthermore, we propose that this strategy can be deployed to unravel myriad confounding pathologies displaying variable expressivity.
    Keywords: Arrhythmias ; Cardiology ; Genetics ; Ion Channels ; Ips Cells ; Mutation ; Long Qt Syndrome -- Genetics ; Monomeric Gtp-Binding Proteins -- Genetics ; Potassium Channels, Tandem Pore Domain -- Genetics
    ISSN: 00219738
    E-ISSN: 1558-8238
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  • 5
    Language: English
    In: Frontiers in Immunology, Nov 3, 2016
    Description: Immunology is the science of biological warfare between the defenses of our immune systems and offensive pathogenic microbes and cancers. Over the course of his scientific career Eckhard R. Podack made several seminal discoveries that elucidated key aspects of this warfare at a molecular level. When Eckhard joined the complement laboratory of Müller-Eberhard in 1974 he was fascinated by two questions: (1) What is the molecular mechanism by which complement kills invasive bacteria? and (2) Which one of the complement components is the killer molecule? Eckhard’s quest to answer these questions would lead to the discovery C9 and later, two additional pore-forming killer molecules of the immune system. Here is a brief account of how he discovered poly-C9, the pore-forming protein of complement in blood and interstitial fluids; Perforin-1, expressed by natural killer cells (NK) and cytotoxic T lymphocytes; and Perforin-2 (MPEG1), expressed by all cell types examined to date. All three killing systems are crucial for our survival and health.
    Keywords: Cancer – Care and Treatment ; Immune System – Health Aspects ; Microorganisms – Analysis ; Pore-Forming Toxins – Dosage and Administration
    ISSN: 1664-3224
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  • 6
    In: PLoS ONE, 2013, Vol.8(12)
    Description: Thermal layering in reservoirs upstream from hydroelectric dams can create temperature gradients in fishways used by upstream migrating adults. In the Snake River, Washington, federally-protected adult salmonids ( Oncorhynchus spp.) often encounter relatively cool water in dam tailraces and lower ladder sections and warmer water in the upstream portions of ladders. Using radiotelemetry, we examined relationships between fish passage behavior and the temperature difference between the top and bottom of ladders (∆T) at four dams over four years. Some spring Chinook salmon ( O. tshawytscha ) experienced ∆T ≥ 0.5 °C. Many summer and fall Chinook salmon and summer steelhead ( O. mykiss ) experienced ∆T ≥ 1.0 °C, and some individuals encountered ΔT 〉 4.0°C. As ΔT increased, migrants were consistently more likely to move down fish ladders and exit into dam tailraces, resulting in upstream passage delays that ranged from hours to days. Fish body temperatures equilibrated to ladder temperatures and often exceeded 20°C, indicating potential negative physiological and fitness effects. Collectively, the results suggest that gradients in fishway water temperatures present a migration obstacle to many anadromous migrants. Unfavorable temperature gradients may be common at reservoir-fed fish passage facilities, especially those with seasonal thermal layering or stratification. Understanding and managing thermal heterogeneity at such sites may be important for ensuring efficient upstream passage and minimizing stress for migratory, temperature-sensitive species.
    Keywords: Research Article
    E-ISSN: 1932-6203
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  • 7
    Language: English
    In: PLoS ONE, 01 January 2016, Vol.11(3), p.e0152580
    Description: Glycosylated hemoglobin A1c (HbA1c) has been proposed as an independent predictor of long-term prognosis in pulmonary arterial hypertension. However, the clinical relevance of HbA1c in patients with operable chronic thromboembolic pulmonary hypertension (CTEPH) remains unknown. The aim of the...
    Keywords: Sciences (General)
    E-ISSN: 1932-6203
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  • 8
    In: Case Reports in Orthopedics, 2017, Vol.2017, 5 pages
    Description: . Patient with a C2 fracture and entrapment of the right vertebral artery in the fracture gap.. Presentation of a case with follow-up until end of treatment.. Case report.. A 25-year-old woman was brought into our emergency room after falling while riding a horse. She complained of pain in the cervical spine. Clinical examinations showed at the upper cervical spine and painful impairment of the mobility of the neck, with no signs of neurological impairment. Radiological diagnostics revealed a traumatic C2/3 spondylolisthesis. A computer tomography (CT) angiographic scan showed a dislocation of the right vertebral artery into the fracture gap without injury to the artery. Open reduction and osteosynthesis were considered of too high risk. Therefore, we conducted fracture treatment with closed reduction and halo fixation. After removal of the halo fixator, the patient was given a soft cervical collar and was advised to rest for additional 6 weeks before beginning gradual activity.. Conventional follow-up revealed osseous consolidation and a CT angiographic scan showed consistent blood flow to the artery.. Halo fixation was a safe and effective therapy strategy in the case of vertebral artery entrapment after traumatic C2 spondylolisthesis.
    Keywords: Cat Scans -- Usage ; Spondylolisthesis -- Diagnosis ; Spondylolisthesis -- Care And Treatment ; Internal Fixation -- Usage ; Emergency Medical Services -- Management;
    ISSN: 2090-6749
    E-ISSN: 2090-6757
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  • 9
    In: Nature Genetics, 2015
    Description: The function of the majority of genes in the mouse and human genomes remains unknown. The mouse embryonic stem cell knockout resource provides a basis for the characterization of relationships between genes and phenotypes. The EUMODIC consortium developed and validated robust methodologies for the broad-based phenotyping of knockouts through a pipeline comprising 20 disease-oriented platforms. We developed new statistical methods for pipeline design and data analysis aimed at detecting reproducible phenotypes with high power. We acquired phenotype data from 449 mutant alleles, representing 320 unique genes, of which half had no previous functional annotation. We captured data from over 27,000 mice, finding that 83% of the mutant lines are phenodeviant, with 65% demonstrating pleiotropy. Surprisingly, we found significant differences in phenotype annotation according to zygosity. New phenotypes were uncovered for many genes with previously unknown function, providing a powerful basis for hypothesis generation and further investigation in diverse systems.
    Keywords: Gene Mutation -- Identification And Classification ; Gene Mutation -- Health Aspects ; Human Genome -- Research;
    ISSN: 1061-4036
    E-ISSN: 1546-1718
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