Acta Crystallographica Section D, 01 February 2010, Vol.66(2), pp.176-180
Endosialidase NF (endoNF) is a bacteriophage‐derived endosialidase that specifically degrades α‐2,8‐linked polysialic acid. The structure of a new crystal form of endoNF in complex with sialic acid has been refined at 0.98 Å resolution. The 210 kDa homotrimeric multi‐domain enzyme displays outstanding stability and resistance to SDS. Even at atomic resolution, only a minor fraction of side chains possess alternative conformations. However, multiple conformations of an active‐site residue imply that it has an important catalytic function in the cleavage mechanism of polysialic acid.
Endosialidase Nf ; Polysialic Acid ; Atomic Resolution