Acta Crystallographica Section D, November 2004, Vol.60(11), pp.2019-2021
Regucalcin is a novel calcium ion (Ca) binding protein that does not contain an EF‐hand motif as a Ca‐binding domain and has been demonstrated to play a multi‐functional role in many cell types. Human liver regucalcin, consisting of 299 amino‐acid residues, was overexpressed in , purified and crystallized by the vapour‐diffusion method in the presence of polyethylene glycol 4000 as a precipitant. A native crystal diffracted to 2.8 Å with synchrotron radiation and belongs to space group 2, with unit‐cell parameters = 64.87, = 52.52, = 86.38 Å, β = 99.86°. Two molecules most probably exist in the asymmetric unit, corresponding to = 2.2 Å Da. Heavy‐atom derivative data were collected and the Pb derivative showed one high‐occupancy site per molecule.
Regucalcin ; Calcium‐Binding Proteins.