Infection and Immunity, 2001, Vol. 69(3), p.1483
Haemophilus ducreyi expresses several putative virulence factors in vitro. Isogenic mutant-to-parent comparisons have been performed in a human model of experimental infection to examine whether specific gene products are involved in pathogenesis. Several mutants (momp, ftpA, losB, lst, cdtC, and hhdB) were as virulent as the parent in the human model, suggesting that their gene products did not play a major role in pustule formation. However, we could not exclude the possibility that the gene of interest was not expressed during the initial stages of infection. Biopsies of pustules obtained from volunteers infected with H. ducreyi were subjected to reverse transcription-PCR. Transcripts corresponding to momp, ftpA, losB, lst, cdtB, and hhdA were expressed in vivo. In addition, transcripts for other putative virulence determinants such as ompA2, tdhA, lspA1, and lspA2 were detected in the biopsies. These results indicate that although several candidate virulence determinants are expressed during experimental infection, they do not have a major role in the initial stages of pathogenesis.
Haemophilus Ducreyi ; Haemophilus Ducreyi ; Volunteers ; Mutants ; Virulence ; Gene Expression ; Reverse Transcription ; Polymerase Chain Reaction ; Volunteers ; Mutants ; Virulence ; Gene Expression ; Reverse Transcription ; Polymerase Chain Reaction ; Momp Gene ; Ftpa Gene ; Losb Gene ; Lst Gene ; Cdtc Gene ; Hhdb Gene ; Ompa2 Gene ; Tdha Gene ; Lspa1 Gene ; Lspa2 Gene ; Momp Gene ; Ftpa Gene ; Losb Gene ; Lst Gene ; Cdtc Gene ; Hhdb Gene ; Ompa2 Gene ; Tdha Gene ; Lspa1 Gene ; Lspa2 Gene ; Antigenic Properties and Virulence ; Bacterial Genetics ; Cdtc Gene ; Ftpa Gene ; Hhdb Gene ; Losb Gene ; Lspa1 Gene ; Lspa2 Gene ; Lst Gene ; Momp Gene ; Ompa2 Gene ; Tdha Gene;
View this record in ASM
View full text in ASM (subscribers only)