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  • Article  (4)
  • Vogel, J.  (4)
  • Springer (CrossRef)  (4)
  • 1
    Language: German
    In: BIOspektrum, 2017, Vol.23(5), pp.525-528
    Description: Single-cell RNA-seq has revolutionized our understanding of cellular heterogeneity in a wide spectrum of biological processes. Pioneering studies suggest complex mechanisms and division of labour employed by the bacterial pathogen Salmonella to subvert host cell functions during infection. Here we describe some of our findings from single-cell RNA-seq studies that have revealed and characterized heterogeneity between individual cells infected with this intracellular pathogen, and outline future challenges in this burgeoning field.
    Keywords: Life Sciences ; Life Sciences, General ; Biochemistry, General ; Human Genetics ; Microbiology ; Pharmacology/Toxicology ; Sciences (General);
    ISSN: 0947-0867
    E-ISSN: 1868-6249
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  • 2
    Language: English
    In: Analytical and bioanalytical chemistry, October 2009, Vol.395(3), pp.719-27
    Description: To establish planar biomimetic membranes across large scale partition aperture arrays, we created a disposable single-use horizontal chamber design that supports combined optical-electrical measurements. Functional lipid bilayers could easily and efficiently be established across CO(2) laser micro-structured 8 x 8 aperture partition arrays with average aperture diameters of 301 +/- 5 microm. We addressed the electro-physical properties of the lipid bilayers established across the micro-structured scaffold arrays by controllable reconstitution of biotechnological and physiological relevant membrane peptides and proteins. Next, we tested the scalability of the biomimetic membrane design by establishing lipid bilayers in rectangular 24 x 24 and hexagonal 24 x 27 aperture arrays, respectively. The results presented show that the design is suitable for further developments of sensitive biosensor assays, and furthermore demonstrate that the design can conveniently be scaled up to support planar lipid bilayers in large square-centimeter partition arrays.
    Keywords: Membranes, Artificial ; Biomimetics -- Instrumentation ; Lipid Bilayers -- Chemistry ; Membrane Proteins -- Chemistry ; Peptides, Cyclic -- Chemistry
    ISSN: 16182642
    E-ISSN: 1618-2650
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  • 3
    Language: English
    In: Theory of Computing Systems, 2003, Vol.36(4), pp.375-386
    Description: In 1977 Young proposed a voting scheme that extends the Condorcet Principle based on the fewest possible number of voters whose removal yields a Condorcet winner. We prove that both the winner and the ranking problem for Young elections is complete for \p || NP , the class of problems solvable in polynomial time by parallel access to NP. Analogous results for Lewis Carroll's 1876 voting scheme were recently established by Hemaspaandra et al. In contrast, we prove that the winner and ranking problems in Fishburn's homogeneous variant of Carroll's voting scheme can be solved efficiently by linear programming.
    Keywords: Computation ; Elections ; Complexity ; Polynomials ; Linear Programming ; Ranking ; Voters ; Voting ; Computing Milieux (General) (Ci);
    ISSN: 1432-4350
    E-ISSN: 1433-0490
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  • 4
    Language: English
    In: Applied Physics A, 2008, Vol.92(4), pp.853-857
    Description: High-power optical multimode fibers are essential components for materials processing and surgery and can limit the performance of expensive systems due to breakdown at the end faces. The aim of this paper is the determination of laser-induced damage thresholds (LIDT) of fibers (FiberTech) and preforms (Heraeus Suprasil F300). Preforms served as models. They were heated up to maximum temperatures of 1100, 1300 and 1500°C and cooled down to room temperature at rates of 10 K min −1 (oven) and ∼10 5  K min −1 (quenched in air) to freeze in various structural states simulating different conditions similar to a drawing process during the production of fibers. Single- and multi-pulse LIDT measurements were done in accordance with the relevant ISO standards. Nd:YAG laser pulses with durations of 15 ns (1064 nm wavelength) and 8.5 ns (532 nm) at a repetition rate of 10 Hz were used. For the preforms, LIDT values (1-on-1) ranged from 220 to 350 J/cm 2 (1064 nm) and from 80 to 110 J/cm 2 (532 nm), respectively. A multi-pulse impact changed the thresholds to lower values. The LIDT (1064 nm wavelength) of the preforms can be regarded as a lower limit for those of the fibers.
    Keywords: 52.38.Mf ; 79.20.Ds ; 42.81.Qb ; 64.70.P- ; 81.05.Kf
    ISSN: 0947-8396
    E-ISSN: 1432-0630
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