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  • Springer (CrossRef)  (870)
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  • 1
    Language: English
    In: Milan Journal of Mathematics, 2011, Vol.79(1), pp.67-79
    Description: First the connection between conformal mappings and Plateau’s problem is pointed out. Then the relation between minimizers of area and energy under Plateau boundary conditions is discussed (joint work with F. Sauvigny). Finally, generalizations of the mapping theorems of Riemann and Koebe for Riemannian metrics are presented (joint work with H. von der Mosel).
    Keywords: Minimal surfaces ; area ; Dirichlet integral ; Plateau’s problem ; mapping theorems of Riemann and Koebe
    ISSN: 1424-9286
    E-ISSN: 1424-9294
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  • 2
    Language: English
    In: Analytical and Bioanalytical Chemistry, 2010, Vol.397(5), pp.1787-1796
    Description: Fish are a common cause of allergic reactions associated with food consumption, with parvalbumin being the major allergenic protein. Some fish-hypersensitive patients tolerate some fish species while being allergic to others. Reliable detection methods for allergenic fish species in foods are necessary to ensure compliance with food allergen labeling guidelines to protect fish-allergic consumers. The objective of this project was to develop a multi-analyte detection method for the presence of fish in food. Therefore, conserved parvalbumin exon sequences were utilized for the design of universal PCR primers amplifying intron DNA and small regions of exons flanking the enclosed intron from even very distantly related fish species. An assay for the identification of eight fish species was developed using xMAP™ technology with probes targeting species-specific parvalbumin intron regions. Additionally, a universal fish probe was designed targeting a highly conserved exon region located between the intron and the reverse primer region. The universal fish assay showed no cross-reactivity with other species, such as beef, pork, lamb, chicken, turkey, and shrimp. Importantly, with the exception of one notable case with fish in the same subfamily, species-specific detection showed no cross-reactivity with other fish species. Limits of detection for these eight species were experimentally estimated to range from 0.01% to 0.04%, with potential to increase the detection sensitivity. This report introduces a newly developed method for the multiplex identification of at least eight allergenic fish species in food, which could conceivably be extended to detect up to 100 species simultaneously in one sample. Figure Schematic overview of the xMAP™ assay. Amine-modified capture oligonucleotides were covalently coupled to color-coded MagPlex™-C magnetic carboxylated microspheres (Luminex Corporation, TX, USA) using a carbodiimide coupling method described by Fulton et al. [25]. Parvalbuminencoding DNA was amplified with real-time PCR, whereas one of the primers was labeled with biotin. Biotin-labeled DNA strands were hybridized to their corresponding capture oligonucleotides. The fluorophor streptavidin–phycoerythrin (SAPE) was added and bound to the biotin molecules. Reaction tubes containing the magnetic microspheres were put on a magnet, unbound oligonucleotides and SAPE was removed by washing. The reaction mixture was analyzed on the BioPlex 200 analyzer (BioRad, CA, USA). Microspheres were interrogated individually in a rapidly flowing fluid stream as they passed by two separate lasers. A red diode laser excited two fluorochromes contained within the colorcoded microspheres and a green laser excited the reporter fluorochrome (SAPE) bound to the microsphere surface. High-speed digital signal processing classified the microsphere based on its spectral address and quantified the reaction on the surface
    Keywords: Fish allergy ; Parvalbumin ; PCR ; Universal primer ; xMAP
    ISSN: 1618-2642
    E-ISSN: 1618-2650
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  • 3
    Language: English
    In: Analytical and Bioanalytical Chemistry, 2016, Vol.408(17), pp.4475-4483
    Description: The exceptional photophysical properties and the nanometric dimensions of colloidal semiconductor quantum dots (QD) have strongly attracted the bioanalytical community over the last approximately 20 y. In particular, the integration of QDs in the analysis of biological components and interactions, and the related diagnostics using Förster resonance energy transfer (FRET), have allowed researchers to significantly improve and diversify fluorescence-based biosensing. In this TRENDS article, we review some recent developments in QD-FRET biosensing that have implemented this technology in electronic consumer products, multiplexed analysis, and detection without light excitation for diagnostic applications. In selected examples of smartphone-based imaging, single- and multistep FRET, steady-state and time-resolved spectroscopy, and bio/chemiluminescence detection of QDs used as both FRET donors and acceptors, we highlight the advantages of QD-based FRET biosensing for multiplexed and sensitive diagnostics. Graphical Abstract Quantum dots (QDs) can be applied as donors and/or acceptors for Förster resonance energy transfer- (FRET-) based biosensing for multiplexed and sensitive diagnostics in various assay formats
    Keywords: Fluorescence ; Spectroscopy ; Imaging ; Multiplexing ; Nanoparticle ; Immunoassay ; DNA ; RNA ; Smartphone ; BRET ; CRET
    ISSN: 1618-2642
    E-ISSN: 1618-2650
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  • 4
    Language: English
    In: Clinical Social Work Journal, 2016, Vol.44(1), pp.105-113
    Description: Research has increasingly noted that gay male adults are more at risk for developing body image dissatisfaction than other male populations. Body image issues warrant attention, particularly since they have been connected to the development of disordered eating patterns. Studies have often traced gay male body dissatisfaction to various sociocultural elements and phenomena, particularly the media. In fact, various media genres have been implicated as being instrumental in propagating idealized male physiques, which in turn may negatively influence observers. Using objectification theory, this paper aims to review the process by which media imagery are internalized by some gay men and how such internalizations harmfully impact their body image. The clinical implications and treatment of body dissatisfaction will be reviewed in terms of social work practice with gay male populations.
    Keywords: Gay ; Body image ; Media ; Objectification ; Social comparison
    ISSN: 0091-1674
    E-ISSN: 1573-3343
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  • 5
    Language: English
    In: Philosophy & Technology, 2013, Vol.26(4), pp.357-379
    Description: In this contribution, I will argue that the image of a balance is often used to defend the idea of a trade-off. To understand the drawbacks of this line of thought, I will explore the relationship between online security technologies and fundamental rights, notably privacy, nondiscrimination, freedom of speech and due process. After discriminating between three types of online security technologies, I will trace the reconfiguration of the notion of privacy in the era of smart environments. This will lead to an inquiry into the metaphor of the scale, building on the triple test regarding the justification of the limitation of fundamental rights such as privacy. The conclusion will be that in the case of a trade-off, infringing measures will have to be balanced by effective safeguards. No trade-off without balance.
    Keywords: Online security technologies ; Security ; Safety ; Privacy ; Monitoring ; Filtering ; Confidentiality ; Integrity ; Availability ; Cybercrime ; Balance ; Trade-off
    ISSN: 2210-5433
    E-ISSN: 2210-5441
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  • 6
    Language: English
    In: Philosophy & Technology, 2011, Vol.24(4), pp.371-390
    Description: In this article, I will investigate to what extent democracy and the Rule of Law require that ISPs as ‘common carriers’ that provide ‘mere conduit’ pre-empt extensive monitoring of the content they carry. I will trace this duty as a moral duty that is bound up with the framework of constitutional democracy, arguing that such monitoring affords unprecedented data-mining operations that could stifle our account of ourselves as moral agents in the novel infosphere.
    Keywords: ISPs ; Data mining ; Privacy ; Ontological friction ; Ambient Law ; The Rule of Law ; Deep packet inspection ; Data retention ; Number crunching
    ISSN: 2210-5433
    E-ISSN: 2210-5441
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  • 7
    Article
    Article
    Language: German
    In: Mathematische Semesterberichte, 2010, Vol.57(2), pp.163-168
    Description: Byline: S. Hildebrandt (1) Author Affiliation: (1) Drachenfelsstr. 23, 53757, Sankt Augustin, Deutschland Article History: Registration Date: 07/06/2010 Received Date: 01/02/2010 Accepted Date: 08/03/2010 Online Date: 08/07/2010
    ISSN: 0720-728X
    E-ISSN: 1432-1815
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  • 8
    Language: English
    In: Polar Biology, Oct, 2011, Vol.34(10), p.1523(11)
    Description: Byline: Nicole Hildebrandt (1), Melanie Bergmann (1), Rainer Knust (1) Keywords: Lycodes frigidus; Lycodes squamiventer; Zoarcidae; Age; Growth; Hausgarten; Hakon Mosby Mud Volcano; Fram strait; Barents Sea Abstract: Our basic knowledge of the ecology, especially the age and growth of polar deep-sea biota is still scarce. This study provides first data about the age and growth of the two abundant Arctic fish species Lycodes frigidus and Lycodes squamiventer (Zoarcidae). Lycodes frigidus was caught at the deeper parts (1,546--3,576 m depth) of the HAUSGARTEN observatory (HG), west of Svalbard. The congener Lycodes squamiventer was caught at two HG stations (1,273--1,546 m) and at the Hakon Mosby Mud Volcano (HMMV, ~1,250 m), a cold seep in the southwestern Barents Sea. Age was determined by sagittal otolith increment analysis. Growth performance was assessed by fitting age--length data to a von Bertalanffy growth equation. Our data suggest that L. frigidus and L. squamiventer attain maximum ages of 33 and 21 years, respectively. Lycodes squamiventer from the HMMV had significantly higher growth rates and their maximum age and length was slightly lower compared to conspecifics from the shallow HG stations. Von Bertalanffy growth equations were L .sub.t = 58.9 a (1 - e.sup.(-0.042*t)) for L. frigidus, and L .sub.t = 25.3 a (1 - e.sup.(-0.074*t)) and L .sub.t = 24.2 a (1 - e.sup.(-0.099 .sup.* .sup.t)) for L. squamiventer from HG and the HMMV, respectively. A comparison of these data with those of eight other zoarcids indicates that growth performances are correlated with temperature: the higher the annual mean temperatures experienced, the higher the growth rates. However, maximum ages decrease with increasing temperatures. Author Affiliation: (1) Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, 27570, Bremerhaven, Germany Article History: Registration Date: 31/03/2011 Received Date: 24/11/2010 Accepted Date: 30/03/2011 Online Date: 20/04/2011
    Keywords: Volcanoes -- Analysis
    ISSN: 0722-4060
    E-ISSN: 14322056
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  • 9
    Language: English
    In: Environmental Science and Pollution Research, 2012, Vol.19(2), pp.372-378
    Description: Byline: Thomas Hildebrandt (1), Denis Pick (1), Jurgen W. Einax (1) Keywords: Hotspot; Soil sampling; Sampling strategy; Simulation; Uncertainty Abstract: Introduction The pollution of soil and environment as a result of human activity is a major problem. Nowadays, the determination of local contaminations is of interest for environmental remediation. These hotspots can have various toxic effects on plants, animals, humans, and the whole ecological system. However, economical and juridical consequences are also possible, e.g., high costs for remediation measures. Materials and methods In this study three sampling strategies (simple random sampling, stratified sampling, and systematic sampling) were applied on randomly distributed hotspot contaminations to prove their efficiency in term of finding hotspots. The results were used for the validation of a computerized simulation. Results and conclusion This application can simulate the contamination on a field, the sampling pattern, and a virtual sampling. A constant hit rate showed that none of the sampling patterns could reach better results than others. Furthermore, the uncertainty associated with the results is described by confidence intervals. It is to be considered that the uncertainty during sampling is enormous and will decrease slightly, even the number of samples applied was increased to an unreasonable amount. It is hardly possible to identify the exact number of randomly distributed hotspot contaminations by statistical sampling. But a range of possible results could be calculated. Depending on various parameters such as shape and size of the area, number of hotspots, and sample quantity, optimal sampling strategies could be derived. Furthermore, an estimation of bias arising from sampling methodology is possible. The developed computerized simulation is an innovative tool for optimizing sampling strategies in terrestrial compartments for hotspot distributions. Author Affiliation: (1) Department of Environmental Analysis, Institute of Inorganic and Analytical Chemistry, Friedrich Schiller University of Jena, Lessingstrasse 8, 07745, Jena, Germany Article History: Registration Date: 04/07/2011 Received Date: 07/04/2011 Accepted Date: 04/07/2011 Online Date: 21/07/2011 Article note: Responsible editor: Zhihong Xu
    Keywords: Hotspot ; Soil sampling ; Sampling strategy ; Simulation ; Uncertainty
    ISSN: 0944-1344
    E-ISSN: 1614-7499
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  • 10
    Article
    Article
    Language: English
    In: The Visual Computer, 2016, Vol.32(4), pp.445-463
    Description: The same data can be visualized using various visual styles that each is suitable for specific requirements, e.g., 3D geodata visualized using photorealistic, cartographic, or illustrative styles. In contrast to feature-based styling, image-based styling performed in image space at image resolution allows decoupling styling from image generation and output-sensitive, expressive styling. However, leveraging image-based styling is still impeded. No previous approach allows specifying image-based styling expressively with an extensive inventory of composable operators, while providing styling functionality in a service-oriented, interoperable manner. In this article, we present an interactive system for specifying and providing the functionality of image-based styling. As key characteristics, it separates concerns of styling from image generation and facilitates specifying styling as algebraic compositions of high-level operators using a unified 3D model representation. We propose a generalized visualization model, an image-based styling algebra, two declarative DSLs, an operator taxonomy, an operational model, and a standards-based service interface. The approach facilitates expressive specifications of image-based styling for design, description, and analysis and leveraging the functionality of image-based styling in a service-oriented, interoperable, reusable, and composable manner.
    Keywords: Styling ; Image-based representation ; Visualization model ; Domain-specific language ; Taxonomy ; Optimization ; Interoperability ; Service-oriented computing
    ISSN: 0178-2789
    E-ISSN: 1432-2315
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