Kooperativer Bibliotheksverbund

Berlin Brandenburg

and
and

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • SpringerLink  (6)
Type of Medium
Language
Year
  • 1
    Language: English
    In: Experimental & Applied Acarology, 2002, Vol.28(1), pp.203-208
    Description: As already shown, some inducers of the differentiation of promyelocytic cells along the granulocytic pathway, such as dimethylsulphoxide (DMSO) or all- trans retinoic acid, can enhance propagation of granulocytic ehrlichiae in HL-60 cell cultures. This study was conducted to prove whether sodium valproate, a salt of di- n -propylacetic acid (VPA) known to trigger cellular differentiation in several solid and hematopoietic malignancies is similarly efficient in ehrlichial cultures. Two cell lines derived from HL-60, that is, low-passage undifferentiated HL-60 (HL-60F) and high-passage HL-60 spontaneously differentiated towards monocytic phenotype (HL-60J) were grown in RPMI 1640 medium supplemented with 10% FBS. The respective HL-60F and HL-60J IC 50 -values for NaVPA were estimated to be 0.8 and 2.2 mM under these culture conditions; to stimulate the differentiation, the respective doses of 0.3 and 1.2 mM were then applied. When the NaVPA-treated cells of both lines were challenged with an ehrlichial laboratory strain (HGE), maintained in splenectomized NMRI mice, the respective 1–2 and ≤0.1% primary infection rates in HL-60F and HL-60J cultures were observed 3 days post-inoculation. In comparison, only rare (≤0.1%) infected HL-60F and no infected HL-60J cells were recorded under the same experimental conditions in untreated control cultures. HGE continuously propagated in NaVPA-supplemented HL-60F cultures remained infectious to mice at least up to the 95th passage (12 months). NaVPA can thus facilitated continuous propagation of granulocytic ehrlichiae in cell cultures without a substantial loss of infectiveness.
    Keywords: Anaplasma phagocytophilum ; ehrlichiosis ; cell culture ; HL-60 ; sodium valproate
    ISSN: 0168-8162
    E-ISSN: 1572-9702
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    Language: English
    In: In Vitro Cellular & Developmental Biology - Animal, 1992, Vol.28(3), pp.147-148
    Keywords: Biological sciences -- Biology -- Cytology ; Biological sciences -- Biology -- Cytology ; Biological sciences -- Biology -- Cytology ; Biological sciences -- Biology -- Cytology ; Applied sciences -- Laboratory techniques -- Culture techniques ; Physical sciences -- Chemistry -- Chemical compounds ; Physical sciences -- Chemistry -- Chemical compounds;
    ISSN: 0883-8364
    E-ISSN: 1543-706X
    E-ISSN: 2327431X
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    Language: English
    In: In Vitro Cellular & Developmental Biology, 1990, Vol.26(9), pp.841-842
    Keywords: Biology;
    ISSN: 0883-8364
    E-ISSN: 1475-2689
    E-ISSN: 2327431X
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 4
    Language: English
    In: Medical Microbiology and Immunology, 2005, Vol.194(1), pp.55-59
    Description: Intracellular glutathione (GSH) plays an important regulatory role in the host response to viral infections. Replenishment of intracellular GSH is a desirable yet challenging goal, since systemic GSH supplementation is rather inefficient due to a short half-life of GSH in blood plasma. Further, GSH is not taken up by cells directly, but needs to be broken down into amino acids and resynthesized to GSH intracellularly, this process often being impaired during viral infections. These obstacles may be overcome by a novel glutathione derivative S-acetylglutathione (S-GSH), which is more stable in plasma and taken up directly by cells with subsequent conversion to GSH. In the present study, in vitro effects of supplementation with S-GSH or GSH on intracellular GSH levels, cell survival and replication of human herpes simplex virus type 1 (HSV-1) were studied in human foreskin fibroblasts. In addition, in vivo effects of supplementation with S-GSH or GSH on HSV-1-induced mortality were studied in hr/hr mice. In cell culture, viral infection resulted in a significant decrease of intracellular GSH levels. S-GSH efficiently and dose-dependently (5 and 10 mM tested) restored intracellular GSH, and this replenishment was more efficient than with GSH supplementation. In mice, S-GSH, but not GSH, significantly decreased HSV-1-induced mortality ( P 〈0.05). The data suggest that S-GSH is a suitable antiviral agent against HSV-1 both in vitro and in vivo, indicating that this drug may be of benefit in the adjunctive therapy of HSV-1 infections.
    Keywords: Intracellular glutathione ; S-acetylglutathione ; Herpes simplex virus type 1 infection ; Antiviral drugs
    ISSN: 0300-8584
    E-ISSN: 1432-1831
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 5
    Language: English
    In: Journal of tissue culture methods, 1989, Vol.12(2), pp.67-72
    Description: Mouse NCTC clone 929 L (L-929) cells were propagated continuously over 2 yr in protein-free Eagles's minimal essential medium (EMEM). The cells designated L-929-WS were used for quality-control testing of protein-free EMEM as a model for more general medium quality tests. The parameter for the suggested quality control assay was the growth-promoting activity of the medium for L-929-WS cells. As an example of quality tests we assayed the growth promoting activity of EMEM exposed to various conditions of storage time and temperature. We established the sensitivity of the new assay by comparing it to the original cells L-929 grown in EMEM supplemented with 10% fetal bovine serum (FBS). The assay system using L-929-WS cells propagated continuously in protein-free medium proved to be much more sensitive. The sensitivity, however, was abolished by the addition of FBS in a concentration as low as 1% to a culture medium.
    Keywords: protein-free medium ; quality control testing ; growth-promoting activity
    ISSN: 0271-8057
    E-ISSN: 1573-0603
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 6
    Language: English
    In: Medical Microbiology and Immunology, 2004, Vol.193(4), pp.195-203
    Description: Human cytomegalovirus (HCMV) retinitis causing retinal detachment and destruction of the blood-retina barrier is closely related to retinal hemorrhage/coagulation. However, the effects of procoagulants on HCMV (re)activation in retinal cells have not been investigated yet. Therefore, we studied whether thrombin modulates the expression of HCMV immediate early (IE) and late (L) genes in cultured human retinal pigment epithelial cells (RPE). Thrombin specifically stimulated the protease-activated receptor-1 (PAR-1) on RPE and, surprisingly, inhibited basal and 12,0-tetradecanoylphorbol 13-acetate-stimulated HCMV IE gene expression in infected RPE. On the other hand, HCMV strongly induced Sp1 DNA binding activity, which was prevented by thrombin/PAR1-mediated Sp1 hyperphosphorylation. Our data suggest that thrombin/PAR-1 may inhibit Sp1-dependent HCMV replication, which might be an important regulatory mechanism for HCMV persistence and replication in RPE.
    Keywords: Human cytomegalovirus ; Infectious immunity virus ; Retina ; Signal transduction ; Transcription factors
    ISSN: 0300-8584
    E-ISSN: 1432-1831
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. Further information can be found on the KOBV privacy pages