Infection, 2018, Vol.46(2), pp.189-196
To access, purchase, authenticate, or subscribe to the full-text of this article, please visit this link: http://dx.doi.org/10.1007/s15010-017-1088-y Byline: Cihan Papan (1,2), Melanie Meyer-Buehn (1), Gudrun Laniado (1), Thomas Nicolai (1), Matthias Griese (1), Johannes Huebner (1) Keywords: Children; Neonates; Bacterial pneumonia; Bronchoalveolar lavage; Multiplex PCR Abstract: Background Pneumonia is a major healthcare problem. Rapid pathogen identification is critical, but often delayed due to the duration of culturing. Early, broad antibacterial therapy might lead to false-negative culture findings and eventually to the development of antibiotic resistances. We aimed to assess the accuracy of the new application Unyvero P50 based on multiplex PCR to detect bacterial pathogens in respiratory specimens from children and neonates. Methods In this prospective study, bronchoalveolar lavage fluids, tracheal aspirates, or pleural fluids from neonates and children were analyzed by both traditional culture methods and Unyvero multiplex PCR. Results We analyzed specimens from 79 patients with a median age of 1.8 (range 0.01--20.1). Overall, Unyvero yielded a sensitivity of 73.1% and a specificity of 97.9% compared to culture methods. Best results were observed for non-fermenting bacteria, for which sensitivity of Unyvero was 90% and specificity 97.3%, while rates were lower for Gram-positive bacteria (46.2 and 93.9%, respectively). For resistance genes, we observed a concordance with antibiogram of 75% for those specimens in which there was a cultural correlate. Conclusions Unyvero is a fast and easy-to-use tool that might provide additional information for clinical decision making, especially in neonates and in the setting of nosocomial pneumonia. Sensitivity of the PCR for Gram-positive bacteria and important resistance genes must be improved before this application can be widely recommended. Author Affiliation: (1) 0000 0004 1936 973X, grid.5252.0, University Children's Hospital at Dr. von Haunersches Kinderspital, Ludwig Maximilians University, Lindwurmstr. 4, 80337, Munich, Germany (2) 0000 0001 2190 4373, grid.7700.0, Pediatric Infectious Diseases, Medical Faculty Mannheim, University Children's Hospital Mannheim, Heidelberg University, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany Article History: Registration Date: 22/10/2017 Received Date: 04/08/2017 Accepted Date: 21/10/2017 Online Date: 31/10/2017 Article note: Electronic supplementary material The online version of this article (https://doi.org/10.1007/s15010-017-1088-y) contains supplementary material, which is available to authorized users.
Children ; Neonates ; Bacterial pneumonia ; Bronchoalveolar lavage ; Multiplex PCR
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