Journal of Pharmaceutical Sciences, May 1978, Vol.67(5), pp.627-630
To evaluate drug‐protein binding, a sensitive method for the determination of ibuprofen in submilliliter amounts of serum was required. A specific and highly sensitive procedure, based on benzene extraction of the acidified specimen, TLC of the benzene extract residue, formation of the pentafluorobenzyl esters of the materials eluted from the thin‐layer chromatogram, and quantification of the pentafluorobenzyl esters by GLC, was developed. Utilizing electron‐capture detection, the method is sensitive to 0.1 μg of ibuprofen/0.1 ml of serum. Statistical analyses indicated an average recovery of 97.7% with a standard deviation of ±7.3%. Mass spectrometric analysis, in conjunction with GLC, confirmed the specificity of the method for the intact drug. The procedure was applied successfully to drug absorption and drug‐protein binding studies in humans.
Ibuprofen—Electron-Capture Glc Analysis in Serum, Time Course of Protein Binding in Humans ; Glc, Electron Capture—Analysis, Ibuprofen in Serum ; Protein Binding—Ibuprofen in Humans, Time Course Studied Using Electron-Capture Glc Analysis in Serum ; Anti-Inflammatory Agents—Ibuprofen, Electron-Capture Glc Analysis in Serum, Time Course of Protein Binding in Humans ; Pharmacy, Therapeutics, & Pharmacology
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