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Berlin Brandenburg

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  • Wiley (CrossRef)  (78)
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  • 1
    In: Transplant International, December 1994, Vol.7, pp.684-684
    ISSN: 0934-0874
    E-ISSN: 1432-2277
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  • 2
    In: New Phytologist, January 2017, Vol.213(2), pp.714-726
    Description: Photosystem I (PSI) is a pigment protein complex catalyzing the light‐driven electron transport from plastocyanin to ferredoxin in oxygenic photosynthetic organisms. Several PSI subunits are highly conserved in cyanobacteria, algae and plants, whereas others are distributed differentially in the various organisms. Here we characterized the structural and functional properties of PSI purified from the heterokont alga Nannochloropsis gaditana, showing that it is organized as a supercomplex including a core complex and an outer antenna, as in plants and other eukaryotic algae. Differently from all known organisms, the N. gaditana PSI supercomplex contains five peripheral antenna proteins, identified by proteome analysis as type‐R light‐harvesting complexes (LHCr4‐8). Two antenna subunits are bound in a conserved position, as in PSI in plants, whereas three additional antennae are associated with the core on the other side. This peculiar antenna association correlates with the presence of PsaF/J and the absence of PsaH, G and K in the N. gaditana genome and proteome. Excitation energy transfer in the supercomplex is highly efficient, leading to a very high trapping efficiency as observed in all other PSI eukaryotes, showing that although the supramolecular organization of PSI changed during evolution, fundamental functional properties such as trapping efficiency were maintained.
    Keywords: Electron Microscopy ; Heterokonta ; Light‐Harvesting Complex ; Nannochloropsis ; Photosynthesis ; Photosynthetic Apparatus ; Photosystem
    ISSN: 0028-646X
    E-ISSN: 1469-8137
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  • 3
    Language: English
    In: Biotechnology Journal, August 2012, Vol.7(8), pp.1002-1007
    Description: The stability of therapeutic antibodies during downstream processing and storage is important for functionality and quality. To determine functional antibody performance, the UNIchip high‐density protein microarray with 384 recombinant antigenic targets was developed; this allows characterization of antibody specificity by generating standardized quantitative binding profiles. In this study, we used UNIchip to test the efficacy of a novel protein stabilizing and protecting solution (SPS) to preserve the binding specificity and binding strength of a therapeutic anti‐TNF‐α antibody (Adalimumab; Humira). Our results show that reconstituted SPS‐formulated and lyophilized Adalimumab elicits significantly less off‐target activity after reconstitution and preserves binding strength even after six weeks of storage at 40°C compared with Adalimumab that underwent the same treatment with the original formulation. By means of UNIchip, we were able to confirm the protein stabilizing effects of SPS as shown by preserved antibody functionality. Stabilization and protecting solution (SPS) enables improvement of antibody binding profiles even after thermal stress and prolonged storage. Results from the current study demonstrate that SPS‐formulated antibodies exhibit better antigen binding profiles (specificity and binding intensity) after lyophilization, thermal stress, and reconstitution as shown by means of a protein microchip array.
    Keywords: Antibody ; Diagnostics ; Microarray ; Proteins ; Stability
    ISSN: 1860-6768
    E-ISSN: 1860-7314
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  • 4
    In: Plant Journal, September 2019, Vol.99(5), pp.877-894
    Description: Phosphorylation dynamics of 3 were investigated in by quantitative proteomics and genetic engineering. 3 protein expression and phosphorylation were induced in high light. Our data revealed synergistic and dynamic N‐terminal 3 phosphorylation. Phosphorylated and nonphosphorylated 3 associated with ‐ supercomplexes. The phosphorylation status of 4 was closely linked to the phosphorylation of multiple sites at the N‐terminus of 3, indicating that 3 phosphorylation may operate as a molecular switch modulating 4 phosphorylation, which in turn is important for ‐ disassembly. Notably, 3 phosphorylation diminished under prolonged high light, which coincided with onset of . Hierarchical clustering of significantly altered proteins revealed similar expression profiles of 3, , and . This finding indicated the existence of a functional link between 3 protein abundance and phosphorylation, photosynthetic electron flow, and the oxidative stress response. We analyzed light‐dependent phosphorylation of LHCSR3 and LHCB4 by quantitative proteomics, genetic engineering, and functional measurements. N‐terminal LHCSR3 phosphorylation is light dependent and modulates phosphorylation of LHCB4 and the association of LHCSR3 with photosynthetic multi‐protein complexes. Our data revealed the functional importance of LHCSR3 phosphorylation, pointing to unforeseen functions in algal photosynthesis.
    Keywords: Photosynthesis ; Light Harvesting ; High Light Stress ; Protein Phosphorylation
    ISSN: 0960-7412
    E-ISSN: 1365-313X
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  • 5
    Language: English
    In: Microsurgery, May 2013, Vol.33(4), pp.287-296
    Description: Intraoperative near‐infrared indocyanine‐green (ICG) angiography enables the visualization of microvascular perfusion and may help in the early detection of complications. The purpose of the present study was to examine whether the effect of microvascular stenoses can be quantitatively assessed by analysis of ICG‐angiography in a microvascular model. Graded stenoses and total vessel occlusion of the carotid, aorta, and femoral arteries were created in 25 Wistar rats. Stenoses were graded to reduce arterial flow by 25%, 50%, 75%, and 100% of baseline flow as measured by transit‐time flowmeter analyzing the emission signal of the ICG detected and investigated by the mathematical software tool (FLOW 800). ICG angiography was performed to assess vessel perfusion and flow curves were analyzed and correlated with the stenosis rate. A total of 576 investigations were performed. The area under the curve ( 〈 0.001), first and second maximum ( 〈 0.001), and the maximum slope to the first maximum ( 〈 0.001) were found to be of high prognostic value in evaluating the different flow patterns. Differences were displayed in comparisons by the maximum intensity of the ICG‐concentrations. The maximum slope to the second maximum was found to be predictive in selected vessel types, and specific changes of the flow curve were found to indicate compromised vascular flow. The FLOW 800 tool applied for ICG angiography has shown to be a quick and reliable method for assessing blood flow in vessels in this study. The dynamic assessment of the ICG signal allows reliable identification of microanastomotic complications with the described parameters. © 2013 Wiley Periodicals, Inc. Microsurgery, 2013.
    Keywords: Blood Flow ; Flow (Dynamics) ; Angiography;
    ISSN: 0738-1085
    E-ISSN: 1098-2752
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  • 6
    In: Biotechnology Journal, July 2018, Vol.13(7), pp.n/a-n/a
    Description: Trastuzumab is a recombinant, humanized monoclonal antibody glycoprotein that selectively targets the extracellular domain of human epidermal growth factor receptor 2 protein (Her2) and is an approved anticancer drug. In this study, the authors demonstrate that amino acid‐based advanced formulation development enables ideal balancing between high physical and chemical stability of highly concentrated therapeutic antibody formulations with low viscosities using the model antibody trastuzumab. This work is of significant importance for the manufacturing of stable highly concentrated therapeutic antibodies.
    Keywords: Antibodies ; Biotherapeutics ; Chromatography ; Medical Biotechnology ; Protein Aggregation
    ISSN: 1860-6768
    E-ISSN: 1860-7314
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  • 7
    In: Wound Repair and Regeneration, September 2011, Vol.19(5), pp.597-607
    Description: The pathophysiology leading to delayed wound healing is complex and efficient therapeutic approaches for accelerated wound healing currently do not exist. We developed a novel drug‐eluting platform for the potential use in wound dressings. Here, we report on the potential of eluting ascorbic acid‐2‐phosphate (‐2), a highly stable variant of ascorbic acid, to induce angiogenesis and to promote collagen synthesis by fibroblasts. The drug‐eluting platform device () consists of biocompatible polymeric layers comprising polyethylene terephtalate, polyvinyl alcohol (), and polyurethane with as the solvent for ‐2. The angiogenic potential of ‐2 was evaluated in the endothelial cell tube formation assay () and in the chorion allantoic membrane () model. Collagen synthesis by ‐2‐stimulated fibroblasts was determined by irius ed staining. ‐2 significantly induced angiogenesis in five independent and assays and induced collagen synthesis in two different fibroblast cell lines. The eluting kinetics of ‐2 was determined by the ultraviolet anorop method and the functional 2,2′‐Azinobis‐(3‐ethylbenzthiazolin‐6‐sulfonic acid) method. Eluting profiles showed a continuous release in the range of biologically effective concentrations 〉10 days. This is the first report showing the proangiogenic‐ and collagen‐promoting features of ‐2. loaded with ‐2 ought to be further evaluated as wound dressings or as supplementary pads for topical treatment of delayed wound healing in preclinical studies.
    Keywords: Drug Delivery Systems ; Angiogenesis Inducing Agents -- Pharmacology ; Ascorbic Acid -- Analogs & Derivatives ; Neovascularization, Physiologic -- Drug Effects ; Wound Healing -- Drug Effects;
    ISSN: 1067-1927
    E-ISSN: 1524-475X
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  • 8
    In: Plant Journal, February 2008, Vol.53(4), pp.691-704
    Description: The Metabolomics Standards Initiative (MSI) has recently released documents describing minimum parameters for reporting metabolomics experiments, in order to validate metabolomic studies and to facilitate data exchange. The reporting parameters encompassed by MSI include the biological study design, sample preparation, data acquisition, data processing, data analysis and interpretation relative to the biological hypotheses being evaluated. Herein we exemplify how such metadata can be reported by using a small case study – the metabolite profiling by GC‐TOF mass spectrometry of leaves from a knockout allele of the gene At1g08510 in the Wassilewskija ecotype. Pitfalls in quality control are highlighted that can invalidate results even if MSI reporting standards are fulfilled, including reliable compound identification and integration of unknown metabolites. Standardized data processing methods are proposed for consistent data storage and dissemination via databases.
    Keywords: Gc/Ms ; Standard Operating Procedure ; Abiotic Stress ; Wounding ; Binbase ; Setupx
    ISSN: 0960-7412
    E-ISSN: 1365-313X
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  • 9
    Language: English
    In: Medicinal Research Reviews, May 2005, Vol.25(3), pp.331-342
    Description: Systemically applied agents to modulate the Fas/FasL system, e.g., by stimulation of Fas on activated leukocytes or tumor cells failed as strategies in immune therapy due to severe toxic effects in the host. Recently, a novel strategy has been developed by using immobilized immune active biologicals in a medical device that may allow immune management without expensive systemic therapy. This review reports on the potential role of Fas/FasL in immune therapy and summarizes current experimental and clinical data with the leukocyte inhibition module (LIM), an immobilized anti‐Fas antibody containing device yet used in extracorporeal blood circulation. This proof of principal may stimulate the development of other devices based on the regulation of Fas/FasL or other targets relevant for immune disorders. © 2004 Wiley Periodicals, Inc.
    Keywords: Novel Therapeutic Strategies ; Immune Management ; Apoptosis
    ISSN: 0198-6325
    E-ISSN: 1098-1128
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  • 10
    Language: English
    In: ChemInform, 12 July 2005, Vol.36(28), pp.no-no
    Description: For see ChemInform in Full Text.
    Keywords: Biochemistry ; Review ; Pharmacology ; Medicinal Chemistry ; Vaccines ; Serums
    ISSN: 0931-7597
    E-ISSN: 1522-2667
    Source: John Wiley & Sons, Inc.
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