Reproduction in Domestic Animals, November 2018, Vol.53, pp.103-109
Aim of the study was to examine the effect of deslorelin on uterine tissues of eleven pre‐pubertal bitches aged 4.2 ± 0.6 m. Implants containing placebo (sodium chloride 0.9%; = 4, G I), 4.7 mg ( = 3, ) or 9.4 mg ( = 4, ) deslorelin acetate (Suprelorin; Virbac, France), were administered subcutaneously. Signs of oestrus, vaginal cytology, serum progesterone (P4) and estradiol‐17β (E2) concentrations were monitored until the occurrence of oestrus. Bitches were ovariohysterectomized and sections from the uterine tissue were subjected to immunohistochemistry () for detection of Gn receptor (R), Kisspeptin ()10, Kisspeptin receptor (54), androgen receptor (), oestrogen receptor () α,β, and progesterone receptor (). Tissue sections were scored semi‐quantitatively using an immunoreactivity score () ranging from 0 to 300 (3). Since some animals were ovariohysterectomized before puberty ( = 1 from and = 2 from ), and some in metestrus (all controls and 2 from and each), results from these animals were separately evaluated and compared to the controls. Results: No abnormalities were seen in uterine tissues. Kisspeptin 10 expression was low in all cell types, highest were seen in the vascular endothelial cells. The 54 was mainly detected in the luminal epithelial cells, superficial and deep uterine glands. The expression of 54 and α,β was especially high in bitches operated prepubertally. No difference was observed between the controls and experimental bitches operated in their first metestrus. The and α,β were exclusively expressed in superficial and deep uterine glands and luminal surface epithelial cells. The and Gn‐R expression was negative in all cells of all groups. We conclude that application of 4.7 or 9.4 mg deslorelin at the age of 4 months did not cause uterine disturbances. 54 expression might be influenced by pre‐pubertal deslorelin treatment or the changings related to approaching puberty; the latter is supposed in case of α,β.
Deslorelin ; Dogs ; Gn Rh ; Kisspeptin ; Receptors ; Steroid Hormones ; Uterus