In:
Alcoholism: Clinical and Experimental Research, Wiley, Vol. 38, No. 5 ( 2014-05), p. 1339-1346
Abstract:
Fetal exposure to alcohol can have multiple deleterious effects, including learning disorders and behavioral and executive functioning abnormalities, collectively termed fetal alcohol spectrum disorders. Neonatal mice lacking both calcium‐/calmodulin‐stimulated adenylyl cyclases ( AC s) 1 and 8 demonstrate increased vulnerability to ethanol (EtOH)‐induced neurotoxicity in the striatum compared with wild‐type ( WT ) controls. However, the developmental impact on surviving neurons is still unclear. Methods WT and AC 1/8 double knockout ( DKO ) mice were administered 1 dose of EtOH (2.5 g/kg) between postnatal days 5 to 7 (P5–7). At P30, brains were removed and processed for Golgi–Cox staining. Medium spiny neurons ( MSN s) from the caudate putamen were analyzed for changes in dendritic complexity; number of branches, branch points and terminals, total and average dendritic length; spine density and soma size. Results EtOH significantly reduced the dendritic complexity and soma size in surviving MSN s regardless of genotype without affecting spine density. In the absence of EtOH, genetic deletion of AC 1/8 reduced the dendritic complexity, number of branch points, spine density, and soma size of MSN s compared with WT controls. Conclusions These data indicate that neonatal exposure to a single dose of EtOH is sufficient to cause long‐term alterations in the dendritic complexity of MSN s and that this outcome is not altered by the functional status of AC 1 and AC 8. Therefore, although deletion of AC 1/8 demonstrates a role for the ACs in normal morphologic development and EtOH‐induced neurodegeneration, loss of AC 1/8 activity does not exacerbate the effects of EtOH on dendritic morphology or spine density.
Type of Medium:
Online Resource
ISSN:
0145-6008
,
1530-0277
DOI:
10.1111/acer.2014.38.issue-5
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2046886-6
detail.hit.zdb_id:
3167872-5
SSG:
15,3
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