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  • Annals of the Rheumatic Diseases
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  • 1
    Language: English
    In: Annals of the Rheumatic Diseases, 22 February 2012, Vol.71(Suppl 1), p.A51
    Description: Systemic lupus erythematosus (SLE) is a chronic inflammatory autoimmune disease that affects multiple organs, whose pathology is mainly caused by the augmented interferon (IFN) signaling pathway. The objective of this study was to analyse the particular role of peripheral CD4+T cells in the pathogenesis of SLE by global gene expression profiling. The major focus was set on the comparison of disease-active and -inactive patients either by standard drug treatment or by autologous stem cell transplantation (ASCT) that is assumed to completely reset the autoreactive immunologic memory.
    Keywords: Medicine;
    ISSN: 0003-4967
    ISSN: 00034967
    E-ISSN: 1468-2060
    E-ISSN: 14682060
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  • 2
    Language: English
    In: Annals of the Rheumatic Diseases March 2013, Vol.72(Suppl 1), p.A76
    Description: Multiple Sclerosis (MS) is a chronic inflammatory and demyelinating disease of the CNS with unknown aetiology until now. CD4+ T helper (Th) 1 cells, proinflammatory Th17 cells, CD8+ T cells, B cells, natural killer (NK) cells and denritic cells (DC) are accepted to play in important role in pathogenesis of disease. Lymphocytes of the peripheral blood from multiple sclerosis (MS) patients are characterised by proinflammatory function but robust cell surface markers to distinguish patients from controls are not available until now.
    Keywords: Central Nervous System ; Cell Surface ; Helper Cells ; Autoimmune Diseases ; Statistical Analysis ; Demyelinating Diseases ; Flow Cytometry ; Cd4 Antigen ; Inflammatory Diseases ; Lymphocytes T ; Fluorochromes ; Blood Cells ; Fluorescence ; Cell Number ; Multiple Sclerosis ; Monoclonal Antibodies ; Lymphocytes B ; Leukocytes ; Natural Killer Cells ; Sclerosis ; Peripheral Blood ; Cd8 Antigen ; Immunity ; Inflammation ; Cd62l Protein ; Autoimmunity;
    ISSN: 0003-4967
    ISSN: 00034967
    E-ISSN: 1468-2060
    E-ISSN: 14682060
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  • 3
    Language: English
    In: Annals of the Rheumatic Diseases, 9 March 2010, Vol.69(Suppl 2), p.32
    Description: Cytokines contribute to the host defence by an overall tuning of the immune system. Nevertheless, an excessive and uncontrolled production of proinflammatory cytokines can be responsible for the onset and maintenance of chronic inflammatory diseases like systemic lupus erythematosus (SLE). The cytokine production that accompanies pathophysiological processes of chronic inflammation is reflected within the monocyte transcriptome. The present study was designed to define stimulus-specific expression patterns in tumour necrosis factor α (TNFα), interferon type I (IFNγ) and IFN type II (IFNγ)-stimulated monocytes in vitro, and to use those cytokine signatures to unravel monocyte transcriptome from patients with SLE.
    Keywords: Medicine;
    ISSN: 0003-4967
    ISSN: 00034967
    E-ISSN: 1468-2060
    E-ISSN: 14682060
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  • 4
    Language: English
    In: Annals of the Rheumatic Diseases, 22 February 2012, Vol.71(Suppl 1), p.A2
    Description: Many cytokines are involved in the pathogenesis of chronic rheumatic diseases like systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). To estimate the role of cytokines in these diseases is intriguing considering the fact that they act together within complex cytokine networks. Although various cell types produce cytokines, monocytes are considered as their principal source. Nevertheless, how functions of monocytes are altered in the course of different rheumatic diseases and how cytokines influence these alterations remained largely unknown.
    Keywords: Medicine;
    ISSN: 0003-4967
    ISSN: 00034967
    E-ISSN: 1468-2060
    E-ISSN: 14682060
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  • 5
    Language: English
    In: Annals of the Rheumatic Diseases, June, 2014, Vol.73(6), p.344(2)
    Keywords: Periodic Disease -- Risk Factors ; Periodic Disease -- Genetic Aspects ; Immune Response -- Research ; Autophagy (Cytology) -- Research
    ISSN: 0003-4967
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  • 6
    Language: English
    In: Annals of the Rheumatic Diseases March 2013, Vol.72(Suppl 1), p.A6
    Description: Gene expression profiling experiments using peripheral blood mononuclear cells (PBMCs) revealed a crucial role of type I interferon (IFN) in the pathogenesis of systemic lupus erythematosus (SLE). However, it is almost unknown how particular leukocyte subsets contribute to the overall type I IFN signature described for PBMCs. Furthermore, a detailed analysis of how IFN signatures differ in autoimmune disease from that observed after viral infection is missing so far. Therefore, we compared expression levels of 2442 IFN signature genes in peripheral CD4+ T helper cells and monocyte (Mo) subsets isolated from patients with SLE, healthy donors (ND) and ND vaccinated against yellow fever by global gene expression profiling.
    Keywords: Genomes ; Apoptosis ; DNA Probes ; Helper Cells ; Autoimmune Diseases ; Autoimmunity ; Infection ; Computer Programs ; Cd4 Antigen ; Software ; Peripheral Blood Mononuclear Cells ; Yellow Fever ; Lymphocytes T ; Systemic Lupus Erythematosus ; Monocytes ; Phagocytosis ; Data Processing ; Biomarkers ; Vaccination ; Immunization ; Inflammation ; Databases ; Interferon ; RNA ; Vaccines ; Signal Transduction ; Autoimmunity ; Human Diseases;
    ISSN: 0003-4967
    ISSN: 00034967
    E-ISSN: 1468-2060
    E-ISSN: 14682060
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  • 7
    Language: English
    In: Annals of the Rheumatic Diseases June 2013, Vol.71(Suppl 3), p.648
    Description: Systemic lupus erythematosus (SLE) is a chronic inflammatory autoimmune disease that affects multiple organs, whose pathology is mainly caused by the augmented interferon (IFN) signaling pathway.
    ISSN: 0003-4967
    ISSN: 00034967
    E-ISSN: 1468-2060
    E-ISSN: 14682060
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  • 8
    Language: English
    In: Annals of the Rheumatic Diseases June 2014, Vol.73(Suppl 2), p.344
    Description: Mutations in gene encoding pyrin [1] account for Familial Mediterranean fever FMF, a recessively inherited disease but the high number of heterozygote patients is puzzling and requires additional investigation [2,3].
    ISSN: 0003-4967
    ISSN: 00034967
    E-ISSN: 1468-2060
    E-ISSN: 14682060
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  • 9
    Language: English
    In: Annals of the Rheumatic Diseases, 03/2015, Vol.74(Suppl 1), pp.A33.2-A33
    Description: Background and objectivesGlucose-6-phosphate isomerase (G6PI) immunisation induces acute self-limiting arthritis in DBA/1 mice. Upon depletion of regulatory T cells (Tregs) it is possible to deliberately switch the outcome towards a chronic, destructive and non-remitting disease. Fibroblast-like synoviocytes (FLS) are important effector cells in rheumatoid arthritis (RA). The objective of this work is to unravel differences at the transcriptomic and miRNomic level between FLS from Treg-depleted mice (chronic) and FLS from not-depleted mice (acute).Materials and methodsFLS were isolated from the small joints of G6PI immunised mice either Treg-depleted (chronic) or not-depleted (acute) at day 56 post-immunisation or from healthy mice as a baseline control. A transepitelial resistance assay (MATRIN) was used to test FLS collagen destructive potential, while apoptosis was assessed by serum starvation for 5 days followed by flow cytometry staining. Whole genome expression analysis was realised using mouse 430 2.0 array (Affymetrix). Whole microRNA expression was assessed using TaqMan registered Rodent MicroRNA A Array v2.0 (Applied Biosystems).ResultsFLS from chronic mice at late stages of arthritis show an increased collagen destructive potential and their sensitivity to apoptosis is reduced. Transcriptomic analysis reveals a strong difference in gene expression between FLS from chronic mice and FLS from acute mice. Stimulation with pro-inflammatory cytokines at different time points modulates gene expression differently in cells from chronic mice, enhancing the expression of genes related to cell adhesion, regulation of apoptosis, cell motion and inflammatory response. Comprehensive miRNA analysis shows a panel of overexpressed and down regulated miRNAs that could have an important role in the regulation of arthritis chronicity.ConclusionsFLS from chronic mice are more aggressive and less sensitive to cell death than FLS from acute mice. Whole genome expression analysis confirmed their profound differences at a molecular level. Moreover, stimulation with pro-inflammatory cytokines differentially activates gene expression in FLS from mice with chronic, destructive arthritis. Differences in microRNA expression also confirm these findings. G6PI-induced arthritis mouse model is a useful tool to unravel the role of differentially expressed genes and microRNAs in FLS from chronic mice, in order to discover potential candidates for therapeutic intervention in humans.
    Keywords: Starvation ; Genomes ; Immunoregulation ; Apoptosis ; Mirna ; Joint Diseases ; Animal Models ; Therapeutic Applications ; Immunization ; Effector Cells ; Cell Adhesion ; Inflammation ; Collagen ; Gene Expression ; Flow Cytometry ; Synoviocytes ; Rheumatoid Arthritis ; Arthritis ; Lymphocytes T ; Cytokines ; Autoimmunity ; Methods;
    ISSN: 0003-4967
    E-ISSN: 1468-2060
    Source: CrossRef
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  • 10
    Language: English
    In: Annals of the Rheumatic Diseases March 2011, Vol.70(Suppl 2), p.A86
    Description: Ankylosing spondylitis (AS) is a chronic inflammatory disorder mainly affecting the axial skeleton and belongs to a group of rheumatic diseases known as spondyloarthritides. Anti-tumour necrosis factor (TNF)-α (tumour necrosis factor) biologicals, which aim to block the disease-associated proinflammatory activity of this cytokine, have been successfully applied for treatment of rheumatoid arthritis (RA) and AS as well. Unfortunately, only a subgroup of around 60% of patients is successfully responding to this treatment and up to now, there are no useful biomarkers available for predicting a beneficial clinical response to the cost-intensive TNF-α therapies. The aim of the present study was to monitor treatment of AS patients over time with a multiparametric approach, called cytometric profiling, which allows immunoscoping of hundreds of immunophenotypic parameters at the single cell level. Fifty different monoclonal antibodies were combined to 10 different staining cocktails, which allowed the detection of all major leucocyte populations and their activation state in a few millitres of peripheral blood. Protocols applied include seven colour stainings that will allow the detection of up to 12 parameters. This strategy combines the generation of hypothesis-based and hypothesis-generating knowledge. The huge amount of data generated per measurement made it necessary to develop appropriate bioinformatic solutions for data storage, data normalisation and retrieval of differentially expressed parameters.
    Keywords: Medicine;
    ISSN: 0003-4967
    ISSN: 00034967
    E-ISSN: 1468-2060
    E-ISSN: 14682060
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