Biochemical and Biophysical Research Communications, 2010, Vol.399(4), pp.542-547
► Levels of IL-1β are increased in the pig myocardium after infarction. ► Cultured pig heart cells possess IL-1 receptors. ► IL-1β increases cell proliferation of pig heart cells . ► IL-1β increases MMP-2 and MMP-9 activity in pig heart cells . ► IL-1β may be important for tissue remodelling events after myocardial infarction. After myocardial infarction, elevated levels of interleukins (ILs) are found within the myocardial tissue and IL-1β is considered to play a major role in tissue remodelling events throughout the body. In the study presented, we have established a cell culture model of primary pig heart cells to evaluate the effects of different concentrations of IL-1β on cell proliferation as well as expression and activity of enzymes typically involved in tissue remodelling. Primary pig heart cell cultures were derived from three different animals and stimulated with recombinant pig IL-1β. RNA expression was detected by RT-PCR, protein levels were evaluated by Western blotting, activity of matrix metalloproteinases (MMPs) was quantified by gelatine zymography and cell proliferation was measured using colorimetric MTS assays. Pig heart cells express receptors for IL-1 and application of IL-1β resulted in a dose-dependent increase of cell proliferation ( 〈 0.05 vs. control; 100 ng/ml; 24 h). Gene expression of caspase-3 was increased by IL-1β ( 〈 0.05 vs. control; 100 ng/ml; 3 h), and pro-caspase-3 but not active caspase was detected in lysates of pig heart cells by Western blotting. MMP-2 gene expression as well as enzymatic activities of MMP-2 and MMP-9 were increased by IL-1β ( 〈 0.05 vs. control; 100 ng/ml; 3 h for gene expression, 48 and 72 h for enzymatic activities of MMP-2 and MMP-9, respectively). Our data suggest that IL-1β plays a major role in the events of tissue remodelling in the heart. Combined with our recently published data (Meybohm et al., PLoS One, 2009), the results presented here strongly suggest IL-1β as a key molecule guiding tissue remodelling events after myocardial infarction.
Interleukin-1β ; Cell Proliferation ; Extracellular Matrix Remodelling ; Myocardial Infarction ; Biology ; Chemistry ; Anatomy & Physiology
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