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Berlin Brandenburg

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  • 1
    Language: English
    In: Geomicrobiology Journal, 03 July 2015, Vol.32(6), pp.494-510
    Description: Fe oxidation is often the first chemical reaction that initiates weathering and disaggregation of intact bedrock into regolith. Here we explore the use of pyrosequencing tools to test for evidence that bacteria participate in these reactions...
    Keywords: Biogeochemical Cycling ; Community Structure ; Iron-Oxidizing Bacteria ; Subsurface Microbiology ; Biology ; Chemistry
    ISSN: 0149-0451
    E-ISSN: 1521-0529
    Source: Taylor & Francis (Taylor & Francis Group)
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  • 2
    Language: English
    In: Geomicrobiology Journal, 11/03/2015, pp.00-00
    Description: The natural environment of Geobacter sulfurreducens is oligotrophic and described as limiting in both electron donors and terminal electron acceptors (TEA). In previous studies we examined the effects of long-term TEA (fumarate) limitation, and in this study we examine long-term batch cultures under limiting electron donor. The microorganism survived under long-term electron donor (acetate) starvation, maintaining a stable population of ∼1–2× 10 8 cells mL −1 for 〉650 days. Proteins that varied in abundance with a high level of statistical significance ( p 〈 0.05) for stages between mid-log to survival phase (acetate starved) were identified using iTRAQ based mass spectroscopy. The most highly represented proteins that significantly increased in level in the survival phase cells are generally membrane-associated and are involved in energy metabolism and protein fate. These results document that changes in the outer and cytoplasmic membranes may help G. sulfurreducens survive during starvation through detection and transport of nutrients into the cell. A sizeable portion of the identified proteins with unknown or hypothetical function further suggest that much of the biological process involved in survival have yet to be fully understood. G. sulfurreducens was also able to survive under long-term TEA-starvation conditions with ferric citrate as TEA and maintained a stable population of 1.5–3 × 10 7 cells mL −1 for 〉650 days. We also found that survival phase cells from fumarate-limiting conditions were able to quickly resuscitate and reduce metal such as ferric iron as compared to the mid-log phase cells. Article just-accepted.
    Keywords: Article ; Starvation Phase ; Survival Strategy ; Electron Donor Limitation ; Acetate Starvation ; Itraq ; Lc-Ms/Ms ; Proteomics ; Uranium Bioremediation ; Iron Reduction ; Resuscitation ; Revival ; Ferric Citrate Starvation ; Electron Acceptor Starvation;
    ISSN: 0149-0451
    E-ISSN: 1521-0529
    Source: Informa - Taylor & Francis (via CrossRef)
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  • 3
    Language: English
    In: Geomicrobiology Journal, 01 November 2012, Vol.29(9), pp.792-803
    Description: Microbe-mineral associations in regolith overlying granodiorite bedrock (4.6-4.9 m depth) from the Luquillo Experimental Forest, Puerto Rico, were imaged with confocal scanning laser microscopy at a novel scale of 400X magnification. After adding...
    Keywords: Regolith, Confocal Microscopy, Iron Oxidation, Saprolite, 16s Rrna ; Biology ; Chemistry
    ISSN: 0149-0451
    E-ISSN: 1521-0529
    Source: Taylor & Francis (Taylor & Francis Group)
    Library Location Call Number Volume/Issue/Year Availability
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  • 4
    Language: English
    In: Geomicrobiology Journal, 01 January 2003, Vol.20(1), pp.25-42
    Description: In order to study biosignatures documenting the effects of microbiota on mineral surfaces, microbial matter must be removed without chemically or physically changing the surface. In previous work for analysis by scanning electron microscopy (SEM) we removed soil bacteria from silicate surfaces...
    Keywords: Afm ; Bacteria Removal ; Detergents ; Etch Pits ; Xps ; Biology ; Chemistry
    ISSN: 0149-0451
    E-ISSN: 1521-0529
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  • 5
    Language: English
    In: Geomicrobiology Journal, 11 April 2007, Vol.24(2), pp.79-91
    Description: Marine cyanobacteria have evolved phosphate uptake systems to live where P is limiting. Ultimately, P derives from apatite in rocks but little is known about apatite solubilization by cyanobacteria. Fluorapatite (FAP) was added to a P-free culture medium with and without Anabaena, as well as...
    Keywords: Anabaena ; Apatite ; Phosphate ; Polymeric Substances ; Biology ; Chemistry
    ISSN: 0149-0451
    E-ISSN: 1521-0529
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