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  • 1
    Language: English
    In: International Journal of Urology, 2015, Vol.22(3), p.322(8)
    Description: To purchase or authenticate to the full-text of this article, please visit this link: http://onlinelibrary.wiley.com/doi/10.1111/iju.12679/abstract Byline: Johannes Weller, Manuela Pose, Chris Protzel, Felix Mader, Katrin Porath, Rudiger Kohling, Rudiger Kohling, Rudiger Kohling Objectives To analyze the effect of adenosine on detrusor smooth muscle contraction and to assess age-related changes of adenosine function. Methods Sustained contractions were induced in young (10-30days) and old (〉60days) rat detrusor muscle strips by application of 30mmol/LK.sub.+ and adenosine (0.1-400A[micro]mol/L), which was either applied before raising the K.sub.+ concentration or added to the precontracted muscle strip. Quantitative polymerase chain reaction analyses were used to study adenosine receptor expression in rat and human detrusor specimens. Results Pretreatment with adenosine dose-dependently reduced subsequent K.sub.+-induced contraction in detrusor muscle strips from young rats (half-maximal effect=40A[micro]mol/L). The residual depolarization-induced contraction strength in young tissue was significantly smaller than in tissue from old animals, showing a greater potency of adenosine in young detrusor samples. Likewise, the relaxing effect of adenosine on precontracted detrusor muscle was also significantly more pronounced in young compared with older detrusor. Quantitative polymerase chain reaction showed an age-related downregulation of the adenosine A2B receptor in rat detrusor tissues, which could be confirmed in human detrusor samples. Furthermore, relaxation of both K.sub.+-induced as well as carbachol-induced contraction by the specific A2B receptor agonist BAY 60-6583 was significantly more pronounced in young than in old rats. Conclusions Adenosine powerfully counteracts contraction of detrusor smooth muscle, which is lost in the aging bladder. This is paralleled by an age-dependent transcriptional downregulation of the low-affinity A2B receptor. Hence, this might be pathophysiologically relevant in conditions of raised adenosine concentrations, such as hyperactive bladder contractility.
    ISSN: 0919-8172
    Source: Cengage Learning, Inc.
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  • 2
    Language: English
    In: International journal of urology : official journal of the Japanese Urological Association, March 2015, Vol.22(3), pp.322-9
    Description: To analyze the effect of adenosine on detrusor smooth muscle contraction and to assess age-related changes of adenosine function. Sustained contractions were induced in young (10-30 days) and old (〉60 days) rat detrusor muscle strips by application of 30 mmol/L K(+) and adenosine (0.1-400 µmol/L), which was either applied before raising the K(+) concentration or added to the precontracted muscle strip. Quantitative polymerase chain reaction analyses were used to study adenosine receptor expression in rat and human detrusor specimens. Pretreatment with adenosine dose-dependently reduced subsequent K(+) -induced contraction in detrusor muscle strips from young rats (half-maximal effect = 40 µmol/L). The residual depolarization-induced contraction strength in young tissue was significantly smaller than in tissue from old animals, showing a greater potency of adenosine in young detrusor samples. Likewise, the relaxing effect of adenosine on precontracted detrusor muscle was also significantly more pronounced in young compared with older detrusor. Quantitative polymerase chain reaction showed an age-related downregulation of the adenosine A2B receptor in rat detrusor tissues, which could be confirmed in human detrusor samples. Furthermore, relaxation of both K(+) -induced as well as carbachol-induced contraction by the specific A2B receptor agonist BAY 60-6583 was significantly more pronounced in young than in old rats. Adenosine powerfully counteracts contraction of detrusor smooth muscle, which is lost in the aging bladder. This is paralleled by an age-dependent transcriptional downregulation of the low-affinity A2B receptor. Hence, this might be pathophysiologically relevant in conditions of raised adenosine concentrations, such as hyperactive bladder contractility.
    Keywords: Detrusor ; Isometric Contraction ; Organ Bath ; Real-Time Polymerase Chain Reaction ; Adenosine -- Therapeutic Use ; Adenosine A2 Receptor Agonists -- Therapeutic Use ; Aminopyridines -- Therapeutic Use ; Muscle Contraction -- Drug Effects ; Muscle, Smooth -- Drug Effects ; Receptor, Adenosine A2b -- Metabolism ; Urinary Bladder, Overactive -- Drug Therapy
    ISSN: 09198172
    E-ISSN: 1442-2042
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  • 3
    In: International Journal of Urology, March 2015, Vol.22(3), pp.322-329
    Description: OBJECTIVES: To analyze the effect of adenosine on detrusor smooth muscle contraction and to assess age-related changes of adenosine function.METHODS: Sustained contractions were induced in young (10-30 days) and old (〉60 days) rat detrusor muscle strips by application of 30 mmol/L K(+) and adenosine (0.1-400 µmol/L), which was either applied before raising the K(+) concentration or added to the precontracted muscle strip. Quantitative polymerase chain reaction analyses were used to study adenosine receptor expression in rat and human detrusor specimens.RESULTS: Pretreatment with adenosine dose-dependently reduced subsequent K(+) -induced contraction in detrusor muscle strips from young rats (half-maximal effect = 40 µmol/L). The residual depolarization-induced contraction strength in young tissue was significantly smaller than in tissue from old animals, showing a greater potency of adenosine in young detrusor samples. Likewise, the relaxing effect of adenosine on precontracted detrusor muscle was also significantly more pronounced in young compared with older detrusor. Quantitative polymerase chain reaction showed an age-related downregulation of the adenosine A2B receptor in rat detrusor tissues, which could be confirmed in human detrusor samples. Furthermore, relaxation of both K(+) -induced as well as carbachol-induced contraction by the specific A2B receptor agonist BAY 60-6583 was significantly more pronounced in young than in old rats.CONCLUSIONS: Adenosine powerfully counteracts contraction of detrusor smooth muscle, which is lost in the aging bladder. This is paralleled by an age-dependent transcriptional downregulation of the low-affinity A2B receptor. Hence, this might be pathophysiologically relevant in conditions of raised adenosine concentrations, such as hyperactive bladder contractility.
    Keywords: Detrusor ; Isometric Contraction ; Organ Bath ; Real‐Time Polymerase Chain Reaction
    ISSN: 0919-8172
    E-ISSN: 1442-2042
    Source: John Wiley & Sons, Inc.
    Library Location Call Number Volume/Issue/Year Availability
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