Microbes and Infection, 2006, Vol.8(9), pp.2465-2468
causes the sexually transmitted genital ulcer disease chancroid. In human inoculation experiments, bacteria colocalize with neutrophils and macrophages but remain extracellular. The organism also colocalizes with collagen and fibrin but not with keratinocytes, fibroblasts, laminin, or fibronectin. These relationships are established by 48 h postinoculation and persist through the pustular stage of disease. To extend these observations to the ulcerative stage of disease, and to compare results in the human model with those of natural disease, we obtained biopsies from patients with naturally acquired chancroid. All ulcers were culture positive for and histologically very similar to pustules from the human model. Staining with -specific monoclonal antibodies demonstrated within 5 biopsies. The organism was chiefly found within the granulocytic infiltrate of the ulcer. Dual staining for and eukaryotic tissue components showed that colocalized with neutrophils and fibrin at the ulcerative stage of disease. No bacteria were associated with keratinocytes, fibroblasts, or collagen. Overall, these findings are consistent with results from the human model. This is the first reported study to localize bacteria specifically identified as within naturally acquired chancroid.
Haemophilus Ducreyi ; Chancroid ; Genital Ulcer ; Bacterial Localization ; Biology
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