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  • 1
    Language: English
    In: PROTEOMICS, November 2006, Vol.6(22), pp.6042-6052
    Description: The tuber of potato () is commonly used as a model for underground storage organs. In this study, changes in the proteome were followed from tuberization, through tuber development and storage into the sprouting phase. Data interrogation using principal component analysis was able to clearly discriminate between the various stages of the tuber life cycle. Moreover, five well‐defined protein expression patterns were found by hierarchical clustering. Altogether 150 proteins showing highly significant differences in abundance between specific stages in the life cycle were highlighted; 59 of these were identified. In addition, 50 proteins with smaller changes in abundance were identified, including several novel proteins. Most noticeably, the development process was characterized by the accumulation of the major storage protein patatin isoforms and enzymes involved in disease and defense reactions. Furthermore, enzymes involved in carbohydrate and energy metabolism and protein processing were associated with development but decreased during tuber maturation. These results represent the first comprehensive picture of many proteins involved in the tuber development and physiology.
    Keywords: 2‐De ; Solanum Tuberosum ; Tuber Development
    ISSN: 1615-9853
    E-ISSN: 1615-9861
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  • 2
    Language: English
    In: PROTEOMICS, June 2006, Vol.6(12), pp.3696-3706
    Description: is increasingly acknowledged as one of the best models for studying metal hyperaccumulation in plants. In order to study the mechanisms underlying metal hyperaccumulation, we used proteomic profiling to identify differences in protein intensities among three accessions with pronounced differences in tolerance, uptake and root to shoot translocation of Zn and Cd. Proteins were separated using two‐dimensional electrophoresis and stained with SYPRO Orange. Intensity values and quality scores were obtained for each spot by using PDQuest software. Principal component analysis was used to test the separation of the protein profiles of the three plant accessions at various metal exposures, and to detect groups of proteins responsible for the differences. Spot sets representing individual proteins were analysed with the analysis of variance and non‐parametric Kruskal‐Wallis test. Clearest differences were seen among the accessions, while the effects of metal exposures were less pronounced. The 48 tentatively identified spots represent core metabolic functions ( photosynthesis, nitrogen assimilation, carbohydrate metabolism) as well as putative signalling and regulatory functions. The possible roles of some of the proteins in heavy metal accumulation and tolerance are discussed.
    Keywords: Metal Hyperaccumulator ; Principal Component Analysis ; Statistics ; Thlaspi ; Two‐Dimensional Gel Electrophoresis
    ISSN: 1615-9853
    E-ISSN: 1615-9861
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