The Science of the Total Environment, Oct 15, 2015, Vol.530-531, p.198(11)
To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.scitotenv.2015.05.087 Byline: Mirco Weil, Tobias Mei[sz]ner, Wibke Busch, Armin Springer, Dana Kuhnel, Ralf Schulz, Karen Duis Abstract: For degradation of halogenated chemicals in groundwater Carbo-Iron[R], a composite of activated carbon and nano-sized Fe.sup.0, was developed (Mackenzie et al., 2012). Potential effects of this nanocomposite on fish were assessed. Beyond the contaminated zone Fe.sup.0 can be expected to have oxidized and Carbo-Iron was used in its oxidized form in ecotoxicological tests. Potential effects of Carbo Iron in zebrafish (Danio rerio) were investigated using a 48h embryo toxicity test under static conditions, a 96h acute test with adult fish under semi-static conditions and a 34 d fish early life stage test (FELST) in a flow-through system. Particle diameters in test suspensions were determined via dynamic light scattering (DLS) and ranged from 266 to 497nm. Particle concentrations were measured weekly in samples from the FELST using a method based on the count rate in DLS. Additionally, uptake of particles into test organisms was investigated using microscopic methods. Furthermore, effects of Carbo-Iron on gene expression were investigated by microarray analysis in zebrafish embryos. In all tests performed, no significant lethal effects were observed. Furthermore, Carbo-Iron had no significant influence on weight and length of fish as determined in the FELST. In the embryo test and the early life stage test, growth of fungi on the chorion was observed at Carbo-Iron concentrations between 6.3 and 25mg/L. Fungal growth did not affect survival, hatching success and growth. In the embryo test, no passage of Carbo-Iron particles into the perivitelline space or the embryo was observed. In juvenile and adult fish, Carbo-Iron was detected in the gut at the end of exposure. In juvenile fish exposed to Carbo-Iron for 29 d and subsequently kept for 5d in control water, Carbo-Iron was no longer detectable in the gut. Global gene expression in zebrafish embryos was not significantly influenced by Carbo-Iron. Article History: Received 26 March 2015; Revised 20 May 2015; Accepted 20 May 2015 Article Note: (miscellaneous) Editor: D. Barcelo
Genetic Research – Genetic Aspects ; Genetic Research – Analysis ; Genetic Research – Growth ; DNA Microarrays – Genetic Aspects ; DNA Microarrays – Analysis ; DNA Microarrays – Growth ; Groundwater – Analysis ; Genes – Genetic Aspects ; Genes – Analysis ; Genes – Growth ; Gene Expression – Genetic Aspects ; Gene Expression – Analysis ; Gene Expression – Growth
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