Kooperativer Bibliotheksverbund

Berlin Brandenburg

and
and

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Language: English
    In: Systematic and Applied Microbiology, 1992, Vol.15(4), pp.593-600
    Description: Based on comparative analyses of 16S and 23S ribosomal RNA sequences we have located sites specific for the alpha-, beta-, and gamma-subclasses of Proteobacteria. Short oligodeoxynucleotides complementary to these signature regions were evaluated as potential nucleic acid probes for the differentiation of the major subclasses of Proteobacteria. Hybridization conditions were optimized by the addition of form-amide to the hybridization buffer and high stringency post-hybridization washing. Single-mismatch discrimination of probes was further improved by blocking nontarget probe binding sites with competitor oligonucleotides. Nonisotopic dot-blot hybridization to reference strains demonstrated the expected probe specificities, whole cell hybridization with fluorescent probe derivatives allowed the classification of individual microbial cells. The probes will be useful for determinative studies and for the monitoring of population distribution and dynamics in microbial communities.
    Keywords: Phylogenetic Probes ; 16s and 23s Rrna ; Proteobacteria ; Whole Cell Hybridization ; Single-Mismatch Discrimination ; Biology
    ISSN: 0723-2020
    E-ISSN: 1618-0984
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    Language: English
    In: Systematic and Applied Microbiology, December 1996, Vol.19(4), pp.608-615
    Description: Bacteria with specific metabolic capabilities are required for the degradation of industrial wastewater. In reactors with suspended biomass these organisms may easily be washed out. Reactors with immobilized biomass appear to be better suited to retain those organisms in the system. In order to monitor immobilization efficiency of such a Biof film reactor, the composition of the biof film grown in the reactor has to be examined. In this study a Membrane Biofilm Reactor (MBR) was inoculated with JMP 134 to yield 2,4-dichlorophenoxyacetic acid (2,4-D) degrading biofilms. In a MBR the biofilm is supplied with oxygen through a membrane from the gas compartment and with substrate from the bulk liquid. In situ hybridization of cross sections of the biofilm with 16S rRNA-targeted oligonucleotide probes revealed the spatial distribution of bacterial cells in the biofilm. An oligonucleotide probe specific for the 2,4-degrading strain JMP 134 was developed based on comparative sequence analysis. JMP 134 cells were hardly found directly attached to the membrane, but clusters of them colonized e. g. the testaceous amoebae in the layer close to the membrane. The biofilm itself consisted of three different layers. The bottom layer was characterized by clusters of testaceous amoebae covered with bacterial cells from all groups examined. The base biofilm layer contained organisms of the beta-subclass and - in most cases - fungi. The surface layer exhibited again a higher diversity of bacterial cells and some testaceous amoebae. The overall composition of the biofilm was characterized by a dominance of organisms of the beta-subclass of Proteobacteria. Cells of JMP 134 were found in all three layers, but in different morphological shapes.
    Keywords: In Situ Hybridization ; Oligonucleotide Probes ; A. Eutrophus Jmp 134 ; Biofilm Structure ; Membrane Attached Biofilm ; Biology
    ISSN: 0723-2020
    E-ISSN: 1618-0984
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. Further information can be found on the KOBV privacy pages