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  • 1
    UID:
    almahu_9949251348202882
    Format: 1 online resource (XXI, 786 p. 129 illus., 75 illus. in color.)
    Edition: 2nd ed. 2014.
    ISBN: 1-62703-631-8
    Series Statement: Methods in Molecular Biology, 1072
    Content: Plant Proteomics: Methods and Protocols, Second Edition presents recent advances made in the field of proteomics and their application to plant biology and translational research. In recent years, improvements in techniques and protocols for high-throughput proteomics have been made at all workflow stages, from wet (sampling, tissue and cell fractionation, protein extraction, depletion, purification, separation, MS analysis, quantification) to dry lab (experimental design, algorithms for protein identification, bioinformatics tools for data analysis, databases, and repositories). Divided into nine convenient sections, chapters cover topics such as applications of gel-free, label- or label-free, imaging and targeted approaches to experimental model systems, crops and orphan species, as well as the study and analysis of PTMs, protein interactions, and specific families of proteins, and finally proteomics in translational research. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Plant Proteomics: Methods and Protocols, Second Edition seeks to serve both professionals and novices looking to exploit the full potential of proteomics in plant biology research.
    Note: Bibliographic Level Mode of Issuance: Monograph , Plant Proteomics Methods and Protocols -- From Proteomics to Systems Biology: MAPA, MASS WESTERN, PROMEX and COVAIN as a User-Oriented Platform -- Plant Proteomics: From Genome Sequencing to Proteome Databases and Repositories -- How to Use 2D Gel Electrophoresis in Plant Proteomics -- Standardization of Data Processing and Statistical Analysis in a 2-DE-Based Comparative Plant Proteomics Experiment -- The Expanding Universe of Mass Analyzer Configurations for Biological Analysis -- A Protocol for Protein Extraction from Lipid-Rich Plant Tissues Suitable for Electrophoresis -- Making a Protein Extract in Plant Pathogenic Fungi for Gel- and LC-Based Proteomics -- Plant Proteomics Methods to Reach Low-Abundance Proteins -- Combination of 2DE and LC for Plant Proteomics Analysis -- 2DE Analysis of Forest Tree Proteins Using Fluorescent Labels and Multiplexing -- Differential Plant Proteome Analysis by Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) -- Global Quantitative Proteomics Using Spectral Counting: An Inexpensive Experimental and Bioinformatics Workflow for Deep Proteome Coverage -- Combining Chymotrypsin/trypsin Digestion to Identify Hydrophobic Proteins from Oil Bodies -- Mass Western for Absolute Quantification of Target Proteins and Considerations About the Instrument of Choice -- Selected Reaction Monitoring Mass Spectrometry: A Methodology Overview -- Mass Spectrometry Based Imaging of Metabolites and Proteins -- Using the Yeast Two-Hybrid System to Identify Protein-Protein Interactions -- Modifications of Wheat Germ Cell-Free System for Functional Proteomics of Plant Membrane Proteins -- Arabidopsis Proteomics: A Simple and Standardizable Workflow for Quantitative Proteome Characterization -- Analysis of Rice Proteins Using SDS-PAGE Shotgun Proteomics -- Medicago truncatula Proteomics for Systems Biology: Novel Rapid Shotgun LC-MS Approach for Relative Quantification Based on Full-Scan Selective Peptide Extraxtion (Selpex) -- Soybean Proteomics -- Proteome Analysis of Orphan Plant Species, Fact or Fiction? -- An Improved Detergent-Compatible Gel-Fractionation LC-LTQ-Orbitrap-MS Workflow for Plant and Microbial Proteomics -- Seed Proteomics -- Back to Osborne. Sequential Protein Extraction and LC-MS for Characterization of the Seed Proteome -- Xylem Sap Proteomics -- Suspension Cultured-Plant Cells as a Tool to Analyze the Extracellular Proteome -- Pollen Cultivation and Preparation for Proteome Studies -- Isolation of the Plant Cytosolic Fraction for Proteomic Analysis -- Quantitative Proteomic Analysis of Intact Plastids -- Shotgun Proteomics of Plant Plasma Membrane and Microdomain Proteins Using Nano-LC-MS/MS -- Plant Mitochondrial Proteomics -- Separation of the Plant Golgi Apparatus and Endoplasmic Reticulum by Free-Flow Electrophoresis -- Isolation of Leaf Peroxisomes from Arabidopsis for Organelle Proteomics Analyses -- Proteomics of Field Samples, the Case of the Chernobyl Area -- Differential Proteome and Secretome Analysis During Rice-Pathogen Interaction -- Protein Extraction and Gel-Based Separation Methods to Analyse Responses to Pathogens in Carnation (Dianthus caryophyllus L) -- StageTip-Based HAMMOC, an Efficient and Inexpensive Phosphopeptide Enrichment Method for Plant Shotgun Phosphoproteomics -- Simultaneous Identification and Quantification of Nitrosylation Sites by Combination of Biotin Switch and ICAT Labeling -- Tandem Metal-Oxide Affinity Chromatography for Enhanced Depth of Phosphoproteome Analysis -- N-Glycoprotein Enrichment by Lectin Affinity Chromatography -- Rapid and High-Throughput N-glycomic Analysis of Plant Glycoproteins -- Assay for Proteasome-Dependent Protein Degradation and Ubiquitinated Proteins -- Separation of Membrane Protein Complexes by Native LDS-PAGE -- Identification of Thioredoxin Target Disulfides Using Isotope-Coded Affinity Tags -- Class III Peroxidases -- Proteotyping of Holm oak (Quercus ilex subsp. ballota) Provenances Through Proteomic Analysis of Acorn Flour -- Immunoproteomics Analysis of Food Allergens -- Beer and Wort Proteomics -- Precipitation of Champagne Base Wine Proteins Prior 2D Electrophoresis -- The Minimal Information About a Proteomics Experiment (MIAPE) from the Proteomics Standards Initiative. , English
    Additional Edition: ISBN 1-62703-630-X
    Language: English
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  • 2
    Book
    Book
    Totowa, NJ : Humana Press
    UID:
    gbv_508936799
    Format: XII, 293 S. , graph. Darst.
    ISBN: 1588295613 , 9781588295613
    Series Statement: Methods in molecular biology 358
    Note: Literaturangaben , Metabolite profiling in blood plasma / Oliver Fiehn and Tobias Kind -- Nonsupervised construction and application of mass spectral and retention time index libraries from time-of-flight gas chromatography-mass spectrometry metabolite profiles / Alexander Erban ... [et al.] -- Metabolomic profiling of natural volatiles: headspace trapping: GC/MS / Yury M. Tikunov, Francel W.A. Verstappen, and Robert D. Hall -- Integrative profiling of metabolites and proteins: improving pattern recognition and biomarker selection for systems level approaches / Katja Morgenthal ... [et al.] -- Integrating profiling data: using linear correlation to reveal coregulation of transcript and metabolites / Ewa Urbanczyk-Wochniak, Lothar Willmitzer, and Alisdair R. Fernie -- Visualization and analysis of molecular data / Matthias Scholz and Joachim Selbig -- A gentle guide to the analysis of metabolomic data / Ralf Steuer ... [et al.] -- Capillary electrophoresis-mass spectrometry for metabolomics / Tomoyoshi Soga -- Application of liquid chromatography-mass spectrometry analysis in metabolomics: reversed-phase monolithic capillary chromatography and hydrophilic chromatography coupled to electrospray ionization-mass spectrometry / Vladimir V. Tolstikov, Oliver Fiehn, and Nobuo Tanaka -- High-performance liquid chromatography separations coupled with coulometric electrode array detectors: a unique approach to metabolomics / Bruce S. Kristal ... [et al.] -- Determination of metabolic flux ratios from 13C-experiments and gas chromatography-mass spectrometry data: protocol and principles / Annik Nanchen, Tobias Fuhrer, and Uwe Sauer -- Understanding the roadmap of metabolism by pathway analysis / Stefan Schuster, Axel von Kamp, and Mikhail Pachkov -- Revealing the metabolome of animal tissues using 1H nuclear magnetic resonance spectroscopy / Mark R. Viant -- Nuclear magnetic resonance metabonomics: methods for drug discovery and development / Karl-Heinz Ott and Nelly Aranibar -- Practical aspects of uniform stable isotope labeling of higher plants for heteronuclear NMR-based metabolomics / Jun Kikuchi and Takashi Hirayama
    Language: English
    Subjects: Biology
    RVK:
    RVK:
    RVK:
    Keywords: Metabolom ; Organische Analyse ; Metabolit ; Biochemische Methode
    Author information: Weckwerth, Wolfram 1969-
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  • 3
    UID:
    almafu_9959328899002883
    Format: 1 online resource (xxv, 415 pages) : , illustrations.
    ISBN: 9783527655861 , 3527655867 , 9783527655892 , 3527655891 , 9783527655885 , 3527655883 , 9783527655878 , 3527655875 , 9781299313545 , 129931354X
    Content: Unlike other handbooks in this emerging field, this guide focuses on the challenges and critical parameters in running a metabolomics study, including such often-neglected issues as sample preparation, choice of separation and detection method, recording and evaluating data as well as method validation. By systematically covering the entire workflow, from sample preparation to data processing, the insight and advice offered here helps to clear the hurdles in setting up and running a successful analysis, resulting in high-quality data from every experiment. Based on more than a decade of practical experience in developing, optimizing and validating metabolomics approaches as a routine technology in the academic and industrial research laboratory, the lessons taught here are highly relevant for all systems-level approaches, whether in systems biology, biotechnology, toxicology or pharmaceutical sciences. From the Contents: * Sampling and Sample Preparation in Microbial Metabolomics * Tandem Mass Spectrometry Hyphenated with HPLC and UHPLC for Targeted Metabolomics * GC-MS, LC-MS, CE-MS and Ultrahigh Resolution MS (FTICR-MS) in Metabolomics * NMR-based metabolomics analysis * Potential of Microfluidics and Single Cell Analysis in Metabolomics * Data Processing in Metabolomics * Validation and Measurement Uncertainty in Metabolomic Studies * Metabolomics and its Role in the Study of Mammalian Systems and in Plant Sciences * Metabolomics in Biotechnology and Nutritional Metabolomics and more.
    Note: The Sampling and Sample Preparation Problem in Microbial Metabolomics / Walter M van Gulik, André B Canelas, Reza M Seifar, Joseph J Heijnen -- Tandem Mass Spectrometry Hyphenated with HPLC and UHPLC for Targeted Metabolomics / Gérard Hopfgartner, Emmanuel Varesio -- Uncertainty of Measurement in Quantitative Metabolomics / Raffaele Guerrasio, Christina Haberhauer-Troyer, Stefan Neubauer, Kristaps Klavins, Madeleine Werneth, Gunda Koellensperger, Stephen Hann -- Gas Chromatography and Comprehensive Two-Dimensional Gas Chromatography Hyphenated with Mass Spectrometry for Targeted and Nontargeted Metabolomics / Song Yang, Jamin C Hoggard, Mary E Lidstrom, Robert E Synovec -- LC-MS-Based Nontargeted Metabolomics / Georgios A Theodoridis, Helen G Gika, Ian D Wilson -- The Potential of Ultrahigh Resolution MS (FTICR-MS) in Metabolomics / Franco Moritz, Sara Forcisi, Mourad Harir, Basem Kanawati, Marianna Lucio, Dimitrios Tziotis, Philippe Schmitt-Kopplin -- The Art and Practice of Lipidomics / Koen Sandra, Ruben t'kindt, Lucie Jorge, Pat Sandra -- The Role of CE-MS in Metabolomics / Rawi Ramautar, Govert W Somsen, Gerhardus J de Jong -- NMR-Based Metabolomics Analysis / Andrea Lubbe, Kashif Ali, Robert Verpoorte, Young Hae Choi -- Potential of Microfluidics and Single Cell Analysis in Metabolomics (Micrometabolomics) / Meghan M Mensack, Ryan E Holcomb, Charles S Henry -- Data Processing in Metabolomics / Age K Smilde, Margriet M W B Hendriks, Johan A Westerhuis, Huub C J Hoefsloot -- Metabolic Flux Analysis / Christoph Wittmann, Jean-Charles Portais -- Metabolomics: Application in Plant Sciences / Gaetan Glauser, Julien Boccard, Jean-Luc Wolfender, Serge Rudaz -- Metabolomics and Its Role in the Study of Mammalian Systems / Warwick B Dunn, Mamas Mamas, Alexander Heazell -- Metabolomics in Biotechnology (Microbial Metabolomics) / Marco Oldiges, Stephan Noack, Nicole Paczia -- Nutritional Metabolomics / Hannelore Daniel
    Additional Edition: Print version: Metabolomics in practice. Weinheim : Wiley-VCH, ©2013 ISBN 9783527330898
    Language: English
    Keywords: Electronic books. ; Electronic books. ; Electronic books.
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  • 4
    Online Resource
    Online Resource
    Weinheim :Wiley-VCH Verlag GmbH, | Weinheim :Wiley-VCH,
    UID:
    almafu_9959327918002883
    Format: 1 online resource (448 pages)
    ISBN: 9783527669882 , 3527669884 , 9781299277601 , 1299277608 , 9783527669899 , 3527669892
    Series Statement: Molecular plant biology handbook series
    Content: This is the newest title in the successful?Molecular Plant Biology Handbook Series?. Just like the other titles in the series this new title gives an excellent overview of different approaches and techniques in the field. Contributors are either from ivy-league research institutions or from companies developing new technologies in this dynamic and fast-growing field. With its approach to introduce current techniques in plant metabolomics to a wider audience and with many labs and companies considering using introducing metabolomics for their research, the title meets a growing market. The Kah.
    Note: Central Metabolism. Metabolic Profiling of Plants by GC-MS / Camilla B Hill, Ute Roessner -- Isotopologue Profiling : Toward a Better Understanding of Metabolic Pathways / Wolfgang Eisenreich, Claudia Huber, Erika Kutzner, Nihat Knispel, Nicholas Schramek -- Nuclear Magnetic Resonance Spectroscopy for Plant Metabolite Profiling / Sonia van der Sar, Hye Kyong Kim, Axel Meissner, Robert Verpoorte, Young Hae Choi -- Comprehensive Two-Dimensional Gas Chromatography for Metabolomics / Katja Dettmer, Martin F Almstetter, Christian J Wachsmuth, Peter J Oefner -- MALDI Mass Spectrometric Imaging of Plants / Alees Svatoes, Hans-Peter Mock -- Root and Shoot Metabolomics: Protocol for the Investigation of the Primary Carbon and Nitrogen Metabolism Based on GC-MS / Vlora Mehmeti, Lena Fragner, Stefanie Wienkoop -- Secondary and Lipid Metabolism. Study of the Volatile Metabolome in Plant-Insect Interactions / Georg J F Weingart, Nora C Lawo, Astrid Forneck, Rudolf Krska, Rainer Schuhmacher -- Metabolomics in Herbal Medicine Research / Lie-Fen Shyur, Chiu-Ping Liu, Shih-Chang Chien -- Integrative Analysis of Secondary Metabolism and Transcript Regulation in / Fumio Matsuda, Kazuki Saito -- Liquid Chromatographic-Mass Spectrometric Analysis of Flavonoids / Maciej Stobiecki, Piotr Kachlicki -- Introduction to Lipid (FAME) Analysis in Algae Using Gas Chromatography-Mass Spectrometry / Takeshi Furuhashi, Wolfram Weckwerth -- Multi-Gene Transformation for Pathway Engineering of Secondary Metabolites / Hideyuki Suzuki, Eiji Takita, Kiyoshi Ohyama, Satoru Sawai, Hikaru Seki, Nozomu Sakurai, Toshiya Muranaka, Masao Ishimoto, Hiroshi Sudo, Kazuki Saito, Daisuke Shibata -- Metabolomics and Genomics. Metabolomics-Assisted Plant Breeding / Alexander Herrmann, Nicolas Schauer -- Conducting Genome-Wide Association Mapping of Metabolites / Susanna Atwell, Daniel J Kliebenstein -- Metabolomics and Bioinformatics. Metabolite Clustering and Visualization of Mass Spectrometry Data Using One-Dimensional Self-Organizing Maps / Alexander Kaever, Manuel Landesfeind, Kirstin Feussner, Ivo Feussner, Peter Meinicke -- Metabolite Identification and Computational Mass Spectrometry / Steffen Neumann, Florian Rasche, Sebastian Wolf, Sebastian Bocker -- Using COVAIN to Analyze Metabolomics Data / Xiaoliang Sun, Wolfram Weckwerth -- Mass Spectral Search and Analysis Using the Golm Metabolome Database / Jan Hummel, Nadine Strehmel, Christian Bolling, Stefanie Schmidt, Dirk Walther, Joachim Kopka. , English.
    Additional Edition: Print version: Handbook of plant metabolomics. Weinheim : Wiley-VCH Verlag GmbH 2013 ISBN 3527327770
    Language: English
    Keywords: Electronic books. ; Electronic books. ; Handbooks and manuals. ; Electronic books. ; Electronic books. ; Handbooks and manuals. ; Electronic books. ; Electronic books. ; Handbooks and manuals.
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  • 5
    Online Resource
    Online Resource
    Totowa, NJ :Humana Press :
    UID:
    almahu_9949251249802882
    Format: 1 online resource (308 p.)
    Edition: 1st ed. 2007.
    ISBN: 1-280-83272-X , 9786610832729 , 1-59745-244-0
    Series Statement: Methods in Molecular Biology, 358
    Content: Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic analysis. Leading researchers in the field present protocols for the application of complementary analytical methods, such as gas chromatography-mass spectrometry (GC-MS). Metabolomics: Methods and Protocols contains forward-looking protocols, which provide the essential groundwork for future efforts in elucidating the structure of the unknowns detected in metabolomic studies. Part I of this volume focuses on GC-MS techniques; specifically, attention is given to metabolite profiling in blood plasma, construction and application of mass spectral and retention time index libraries, and metabolomic profiling of natural volatiles. In Part II, emphasis shifts to data integration and data mining techniques, and specific protocols discuss improving pattern recognition and biomarker selection for systems level approaches, and visualization and analysis of molecular and metabolomic data. Parts III, IV, and V examine the coupling of capillary electrophoresis and mass spectrometry, classical reversed phase liquid chromatography-mass spectrometry, and electrochemical detection of metabolites using HPLC separations coupled with coulometric electrode array detectors. In Part VI, protocols for the determination of metabolic flux rates are presented and a theoretical framework for metabolic pathway network analysis is provided. The volume concludes with an examination of cutting-edge NMR protocols.
    Note: Description based upon print version of record. , Metabolome Analysis Using Gas Chromatography Coupled to Mass Spectrometry -- Metabolite Profiling in Blood Plasma -- Nonsupervised Construction and Application of Mass Spectral and Retention Time Index Libraries From Time-of-Flight Gas Chromatography-Mass Spectrometry Metabolite Profiles -- Metabolomic Profiling of Natural Volatiles -- Metabolomics: Data Integration and Data Mining -- Integrative Profiling of Metabolites and Proteins -- Integrating Profiling Data -- Visualization and Analysis of Molecular Data -- A Gentle Guide to the Analysis of Metabolomic Data -- Capillary Electrophoresis Coupled to Mass Spectrometry -- Capillary Electrophoresis-Mass Spectrometry for Metabolomics -- Liquid Chromatography Coupled to Mass Spectrometry for Metabolomics and Structural Elucidation -- Application of Liquid Chromatography-Mass Spectrometry Analysis in Metabolomics -- Electrochemical Detection of Metabolites -- High-Performance Liquid Chromatography Separations Coupled With Coulometric Electrode Array Detectors -- Metabolic Fluxes -- Determination of Metabolic Flux Ratios From 13C-Experiments and Gas Chromatography-Mass Spectrometry Data -- Understanding the Roadmap of Metabolism by Pathway Analysis -- NMR Metabolome Analysis -- Revealing the Metabolome of Animal Tissues Using 1H Nuclear Magnetic Resonance Spectroscopy -- Nuclear Magnetic Resonance Metabonomics -- Practical Aspects of Uniform Stable Isotope Labeling of Higher Plants for Heteronuclear NMR-Based Metabolomics. , English
    Additional Edition: ISBN 1-58829-561-3
    Language: English
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  • 6
    UID:
    gbv_546428002
    Format: 75, 50 S. , graph. Darst.
    Note: enth. auch 21 ZS-Artikel , Potsdam, Univ., kumulat. Habil.-Schr., 2007
    Language: English
    Keywords: Massenspektrometrie ; Hochschulschrift
    Author information: Weckwerth, Wolfram 1969-
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  • 7
    UID:
    b3kat_BV012418152
    Format: 104 S. , Ill., graph. Darst.
    Note: Berlin, Techn. Univ., Diss., 1998
    Language: German
    Keywords: Mycelia sterilia ; Cyclodepsipeptide ; Biosynthese ; Hochschulschrift
    Author information: Weckwerth, Wolfram 1969-
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  • 8
    UID:
    almafu_BV026590453
    Format: Getr. Zählung : , Ill., graph. Darst.
    Note: Potsdam, Univ., Habil.-Schr., 2006
    Language: English
    Keywords: Massenspektrometrie ; Molekularbiologie ; Hochschulschrift
    Author information: Weckwerth, Wolfram 1969-
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  • 9
    UID:
    almafu_BV012418152
    Format: 104 S. : Ill., graph. Darst.
    Note: Berlin, Techn. Univ., Diss., 1998
    Language: German
    Keywords: Mycelia sterilia ; Cyclodepsipeptide ; Biosynthese ; Hochschulschrift
    Author information: Weckwerth, Wolfram 1969-
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  • 10
    Online Resource
    Online Resource
    Weinheim an der Bergstrasse, Germany :Wiley-VCH Verlag GmbH,
    UID:
    almahu_9948317828202882
    Format: 1 online resource (xxv, 415 p.) : , ill.
    Edition: Electronic reproduction. Ann Arbor, MI : ProQuest, 2016. Available via World Wide Web. Access may be limited to ProQuest affiliated libraries.
    Language: English
    Keywords: Electronic books.
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