Format:
201 S.
Edition:
Mikrofiche-Ausg. Ann Arbor, Mich. Univ. Microfilms Internat. 1985 Mikrofiches : 24x [Mikrofiche-Ausg.]
Content:
An investigation to evaluate and determine the source, methodology, survival, control and an animal model for pathogenicity of Campylobacter jejuni was conducted. When retail meats were surveyed for the presence of C. jejuni, the detection rate of this organism was: chicken (35%), lamb stew meat (7.5%), pork chops (2.5%), pork sausage (2.5%), flank steak (2.5%) and ground beef (0.0%). During this survey, many non-Campylobacter colonies grew on the selective agars. The most frequent randomly chosen microorganism from the selective plates were identified as: Bacillus, Citrobacter, Acinetobacter, Leuconostoc, Streptococcus, Proteus, Enterobacter and Pseudomonas. A study was conducted to determine the best mixing method, time and diluent combination for enumerating C. jejuni from skim milk. Results indicate that the blending mechanism, time and diluent are not critical steps in the enumeration of C. jejuni from skim milk. When C. jejuni was inoculated into bovine feces, it survived for 20 days at 25(DEGREES)C and 8 weeks at 4(DEGREES)C. The iodine udder wash was tested for effectiveness of inactivating this organism. This solution can inactivate 10('6) C. jejuni in less than 8 minutes. Hydrogen peroxide was tested for its effect of inactivating C. jejuni in skim and raw milk. At a concentration of 0.05% hydrogen peroxide, a 6 log reduction of this organism will occur by 30 or 60 minutes in either milk depending on the strain tested. The antimicrobial effect of sodium diacetate, oregano, sage and ground cloves on C. jejuni was temperature dependent. The greatest reduction of colony forming units of C. jejuni was observed at 42(DEGREES)C with 25(DEGREES)C being intermediate and 4(DEGREES)C showing the least death for all test chemicals. When 10('6) C. jejuni was spiked into skim milk and 10g beef cubes, a microwave treatment of 50 seconds (temperature of 92(DEGREES)C) and 40 seconds (center temperature of 95.7(DEGREES)C) respectively, was required to inactivate all C. jejuni. When 10('8) C. jejuni suspended in 1% iron dextran was fed to BALB/C mice, these mice became asymptomatic excretors of this organism for up to 5 days. The organism was located in the cecum and the colon.
Note:
Columbus, Ohio, Ohio State Univ., Diss., 1985
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Mikrofiche-Ausg.:
Language:
English
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