Format:
Online-Ressource
ISSN:
1460-2075
Content:
Lariat formation has been studied intensively only with a few self‐splicing group II introns, and little is known about how the numerous diverse introns in plant organelles are excised. Several of these introns have branch‐points that are not a single bulge but are adjoined by A:A, A:C, A:G and G:G pairs. Using a highly sensitive in vivo approach, we demonstrate that all but one of the barley chloroplast introns splice via the common pathway that produces a branched product. RNA editing does not improve domain 5 and 6 structures of these introns. The conserved branch‐point in tobacco rpl16 is chosen even if an adjacent unpaired adenosine is available, suggesting that spatial arrangements in domain 6 determine correct branch‐point selection. Lariats were not detected for the chloroplast trnV intron, which lacks an unpaired adenosine in domain 6. Instead, this intron is released as linear molecules that undergo further polyadenylation. trnV, which is conserved throughout plant evolution, constitutes the first example of naturally occurring hydrolytic group II intron splicing in vivo.
In:
volume:21
In:
number:14
In:
year:2002
In:
pages:3794-3803
In:
extent:10
In:
European Molecular Biology Organization, The EMBO journal, Heidelberg : EMBO Press, 1982-, 21, Heft 14 (2002), 3794-3803 (gesamt 10), 1460-2075
Language:
English
DOI:
10.1093/emboj/cdf359
URN:
urn:nbn:de:101:1-2023110405111789896618
URL:
https://doi.org/10.1093/emboj/cdf359
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023110405111789896618
URL:
https://d-nb.info/1308567308/34
URL:
https://doi.org/10.1093/emboj/cdf359
Bookmarklink
