Format:
Online-Ressource
ISSN:
1860-7314
Content:
Abstract: Recent outbreaks of influenza A highlight the importance of rapid and sufficient supply for pandemic and inter‐pandemic vaccines. Classical manufacturing methods for influenza vaccines fail to satisfy this demand. Alternatively, cell culture‐based production systems and virus‐like particle (VLP)‐based technologies have been established. We developed swine‐origin pandemic H1N1 influenza VLPs consisting of hemagglutinin (A/California/04/2009) and matrix protein. Hemagglutinin and matrix protein were co‐expressed in insect cells by the baculovirus expression system. VLPs were harvested from infection supernatants, purified and used for intraperitoneal immunization of BALB/c mice. Immunization induced high serum antibody titers against A/California/04/2009 as well as hemagglutination inhibiting antibodies. Additionally, we compared VLP production in two different insect cell lines, Sf9 and BTI‐TN5B1‐4 (High Five™). Taken together VLPs represent a potential strategy for the fight against new pandemic influenza viruses.
In:
volume:5
In:
number:1
In:
year:2010
In:
pages:17-23
In:
extent:7
In:
Biotechnology journal, Weinheim : Wiley-VCH, [2006]-, 5, Heft 1 (2010), 17-23 (gesamt 7), 1860-7314
Language:
English
DOI:
10.1002/biot.200900267
URN:
urn:nbn:de:101:1-2023060806215830954966
URL:
https://doi.org/10.1002/biot.200900267
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2023060806215830954966
URL:
https://d-nb.info/1292374241/34
URL:
https://doi.org/10.1002/biot.200900267
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