In:
The EMBO Journal, EMBO, Vol. 36, No. 4 ( 2017-02-15), p. 549-564
Abstract:
image New peptide sensors have been developed to monitor localization and fate of individual LC 3s and GABARAP s of the mATG 8 family, which serve as a central platform for autophagosome biogenesis. Phage display allowed isolation of peptides with LIR ( LC 3‐interacting region) properties that are selective for individual mATG 8 family members. These sensors were optimized by peptide multiplication, charge distribution, and fusion with a membrane recruitment domain ( FYVE ) or oligomerization domain ( PB 1). mATG 8 sensors that recognize specifically GABARAP , GABL 1, GABL 2, and LC 3C, as well as a combined sensor for LC 3A and LC 3B, were successfully used in cells to localize the respective mATG 8s. Endogenous LC 3C and LC 3C‐specific sensor mC h‐ PB 1‐ AS 3_67 are recruited to Salmonella during xenophagy and to mitochondria during mitophagy.
Type of Medium:
Online Resource
ISSN:
0261-4189
,
1460-2075
DOI:
10.15252/embj.201695063
Language:
English
Publisher:
EMBO
Publication Date:
2017
detail.hit.zdb_id:
1467419-1
detail.hit.zdb_id:
586044-1
SSG:
12
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