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  • 1
    Online Resource
    Online Resource
    Amsterdam ; Heidelberg [u.a.] : Academic Press
    UID:
    b3kat_BV042610757
    Format: 1 Online-Ressource
    ISBN: 9780128019344 , 9780128019368
    Series Statement: Methods in enzymology 558
    Language: English
    Subjects: Biology
    RVK:
    Keywords: RNS ; Komplexe ; RNS ; Strukturanalyse ; RNS ; Protein ; Aggregation ; RNS ; Protein ; Interaktion ; RNS ; Struktur ; Dynamik
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Online Resource
    Online Resource
    Waltham, Mass. [u.a.] : Elsevier, Academic Press
    UID:
    gbv_829936947
    Format: Online-Ressource (xxi, 631 S.) , Ill.
    Edition: Online-Ausg.
    Series Statement: Methods in enzymology 558
    Content: This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in RNA folding and dynamics, RNA-protein interactions and large RNPs
    Content: This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in RNA folding and dynamics, RNA-protein interactions and large RNPs
    Note: Includes index
    Additional Edition: ISBN 0128019360
    Additional Edition: ISBN 9780128019344
    Additional Edition: ISBN 9780128019368
    Language: English
    Keywords: RNS ; Komplexe ; Strukturanalyse ; Aufsatzsammlung
    Library Location Call Number Volume/Issue/Year Availability
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  • 3
    UID:
    gbv_1689048603
    Format: 1 Online-Ressource (1 Videodatei, 02:19:33 h) , farbig
    Uniform Title: Merry wives of Windsor
    Content: Imagining that Mistress Ford and Mistress Page have each fallen for him, the fat knight Sir John Falstaff decides to seduce them both, as much for their husbands’ money as for their personal charms. Wise to the old rogue’s tricks, the women turn the tables on him with a series of humiliating assignations and a very damp, extremely smelly laundry basket. Stage director: Christopher Luscombe. Screen director: Robin Lough.
    Language: English
    Keywords: Drama
    Library Location Call Number Volume/Issue/Year Availability
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  • 4
    Book
    Book
    Amsterdam [u.a.] :Elesevier, Acad. Press,
    UID:
    almafu_BV042612704
    Format: XXI, 631 S., [10] Bl. : , Ill., graph. Darst.
    Edition: 1. ed.
    ISBN: 978-0-12-801934-4
    Series Statement: Methods in enzymology 558
    Language: English
    Subjects: Chemistry/Pharmacy
    RVK:
    Keywords: RNS ; Struktur ; Dynamik ; RNS ; Interaktion ; RNS ; Aggregation ; RNS ; Strukturanalyse ; RNS ; Komplexe
    Library Location Call Number Volume/Issue/Year Availability
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  • 5
    Online Resource
    Online Resource
    Amsterdam, [Netherlands] :Academic Press,
    UID:
    edoccha_9958130548802883
    Format: 1 online resource (675 p.)
    Edition: First edition.
    ISBN: 0-12-801936-0 , 0-12-801934-4
    Series Statement: Methods in Enzymology ; Volume 558
    Content: This new volume of 〈i〉Methods in Enzymology〈/i〉 continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in RNA folding and dynamics, RNA-protein interactions and large RNPs.〈br〉〈br〉〈ul〉〈li〉Continues the legacy of this premier serial with quality chapters on structures of large RNA molecules and their complexes〈/li〉〈/ul〉
    Note: Description based upon print version of record. , Front Cover; Structures of Large RNA Molecules and Their Complexes; Copyright; Contents; Contributors; Preface; Section I: RNA Structure and Dynamics; Chapter 1: Native Purification and Analysis of Long RNAs; 1. Introduction; 2. Native Purification of Long Noncoding RNAs; 2.1. Construct design; 2.2. DNA plasmid linearization; 2.3. In vitro transcription of RNA; 2.4. DNase digestion; 2.5. Proteinase K treatment; 2.6. EDTA chelation of divalent ions (optional); 2.7. Buffer exchange and purification; 2.8. Size-exclusion chromatography , 3. Study of the RNA Tertiary Folding by Sedimentation Velocity Analytical Ultracentrifugation3.1. Preparation of samples for a study of RNA folding; 3.2. Assembly of the optical cells, sample loading, and instrument setup; 3.3. Setting up a sedimentation velocity experiment; 3.4. Data analysis; 4. Analysis of the RNA Tertiary Folding by Analytical Size-Exclusion Chromatography; 5. Determination of the Secondary Structure of LncRNAs by Chemical Probing; 5.1. Designing and coupling of primers; 5.2. Generation of sequencing ladders; 5.3. SHAPE reaction; 5.4. DMS reaction , 5.5. Primer extension reaction5.6. Reactions for mobility shift correction; 5.7. Spectral calibration of the instrument; 5.8. Preparation of samples for capillary electrophoresis; 5.9. Data analysis; 5.9.1. Determination of chemical probing reactivity profiles; 5.9.2. Normalization of SHAPE and DMS reactivity profiles; 5.9.3. RNA secondary structure prediction and analysis; Acknowledgments; References; Chapter 2: Characterizing RNA Excited States Using NMR Relaxation Dispersion; 1. Introduction; 2. NMR Relaxation Dispersion; 2.1. Chemical Exchange; 2.2. RD Experiments , 2.3. R1ρ with Low-to-High SL Fields3. General Protocol for Characterizing RNA ESs Using Low-to-High SL Field 13C and 15N Off-Resonance NMR R1ρ and Uniformly...; 3.1. Construct Design; 3.2. Sample Preparation and Purification; 3.3. Protocol for Measuring R1ρ RD in Uniformly Labeled Nucleic Acids; 3.3.1. Calibrating SL Power; 3.3.2. Measurement of 15N R1ρ Data; 3.3.3. Measurement of 13C R1ρ Data; 3.3.4. Trouble Shooting R1ρ; 3.4. Data Analysis; 3.4.1. Fitting Monoexponentials to Obtain R1ρ Values; 3.4.2. Fitting Off-Resonance R1ρ Data Using Algebraic Equations , 3.4.3. Fitting Off-Resonance R1ρ Data Using Bloch-McConnell Equations3.4.4. Determining the Chemical Shifts of the ES; 3.4.5. Plotting RD Profiles; 3.4.6. Estimating Uncertainties in Exchange Parameters; 3.4.7. Kinetic-Thermodynamic Analysis; 3.5. Inferring Structures of RNA ESs Using NMR Chemical Shifts and Secondary Structure Prediction; 3.5.1. 13C and 15N Chemical Shift-Structure Relationships in RNA; 3.5.2. Secondary Structure Prediction; 3.6. Testing Model RNA ESs; 3.6.1. Stabilizing GS and ES Using Mutations; 3.6.2. Stabilizing GS and ES Using Single-Atom Substitutions , 3.6.3. Stabilizing GS and ES by Changing pH , English
    Language: English
    Library Location Call Number Volume/Issue/Year Availability
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  • 6
    Online Resource
    Online Resource
    Amsterdam, [Netherlands] :Academic Press,
    UID:
    almahu_9949697557102882
    Format: 1 online resource (675 p.)
    Edition: First edition.
    ISBN: 0-12-801936-0 , 0-12-801934-4
    Series Statement: Methods in Enzymology ; Volume 558
    Content: This new volume of 〈i〉Methods in Enzymology〈/i〉 continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in RNA folding and dynamics, RNA-protein interactions and large RNPs.〈br〉〈br〉〈ul〉〈li〉Continues the legacy of this premier serial with quality chapters on structures of large RNA molecules and their complexes〈/li〉〈/ul〉
    Note: Description based upon print version of record. , Front Cover; Structures of Large RNA Molecules and Their Complexes; Copyright; Contents; Contributors; Preface; Section I: RNA Structure and Dynamics; Chapter 1: Native Purification and Analysis of Long RNAs; 1. Introduction; 2. Native Purification of Long Noncoding RNAs; 2.1. Construct design; 2.2. DNA plasmid linearization; 2.3. In vitro transcription of RNA; 2.4. DNase digestion; 2.5. Proteinase K treatment; 2.6. EDTA chelation of divalent ions (optional); 2.7. Buffer exchange and purification; 2.8. Size-exclusion chromatography , 3. Study of the RNA Tertiary Folding by Sedimentation Velocity Analytical Ultracentrifugation3.1. Preparation of samples for a study of RNA folding; 3.2. Assembly of the optical cells, sample loading, and instrument setup; 3.3. Setting up a sedimentation velocity experiment; 3.4. Data analysis; 4. Analysis of the RNA Tertiary Folding by Analytical Size-Exclusion Chromatography; 5. Determination of the Secondary Structure of LncRNAs by Chemical Probing; 5.1. Designing and coupling of primers; 5.2. Generation of sequencing ladders; 5.3. SHAPE reaction; 5.4. DMS reaction , 5.5. Primer extension reaction5.6. Reactions for mobility shift correction; 5.7. Spectral calibration of the instrument; 5.8. Preparation of samples for capillary electrophoresis; 5.9. Data analysis; 5.9.1. Determination of chemical probing reactivity profiles; 5.9.2. Normalization of SHAPE and DMS reactivity profiles; 5.9.3. RNA secondary structure prediction and analysis; Acknowledgments; References; Chapter 2: Characterizing RNA Excited States Using NMR Relaxation Dispersion; 1. Introduction; 2. NMR Relaxation Dispersion; 2.1. Chemical Exchange; 2.2. RD Experiments , 2.3. R1ρ with Low-to-High SL Fields3. General Protocol for Characterizing RNA ESs Using Low-to-High SL Field 13C and 15N Off-Resonance NMR R1ρ and Uniformly...; 3.1. Construct Design; 3.2. Sample Preparation and Purification; 3.3. Protocol for Measuring R1ρ RD in Uniformly Labeled Nucleic Acids; 3.3.1. Calibrating SL Power; 3.3.2. Measurement of 15N R1ρ Data; 3.3.3. Measurement of 13C R1ρ Data; 3.3.4. Trouble Shooting R1ρ; 3.4. Data Analysis; 3.4.1. Fitting Monoexponentials to Obtain R1ρ Values; 3.4.2. Fitting Off-Resonance R1ρ Data Using Algebraic Equations , 3.4.3. Fitting Off-Resonance R1ρ Data Using Bloch-McConnell Equations3.4.4. Determining the Chemical Shifts of the ES; 3.4.5. Plotting RD Profiles; 3.4.6. Estimating Uncertainties in Exchange Parameters; 3.4.7. Kinetic-Thermodynamic Analysis; 3.5. Inferring Structures of RNA ESs Using NMR Chemical Shifts and Secondary Structure Prediction; 3.5.1. 13C and 15N Chemical Shift-Structure Relationships in RNA; 3.5.2. Secondary Structure Prediction; 3.6. Testing Model RNA ESs; 3.6.1. Stabilizing GS and ES Using Mutations; 3.6.2. Stabilizing GS and ES Using Single-Atom Substitutions , 3.6.3. Stabilizing GS and ES by Changing pH , English
    Language: English
    Library Location Call Number Volume/Issue/Year Availability
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