In:
Annals of the Rheumatic Diseases, BMJ, Vol. 78, No. 10 ( 2019-10), p. 1398-1404
Abstract:
Familial Mediterranean fever (FMF) can be complicated by AA
amyloidosis (AAA), though it remains unclear why only some patients develop amyloidosis. We examined the gut microbiota composition and
inflammatory markers in patients with FMF complicated or not by AAA. Methods We analysed the gut microbiota of 34 patients with FMF without AAA,
7 patients with FMF with AAA, 19 patients with AAA of another origin, and 26 controls using 16S ribosomal RNA gene sequencing with the
Illumina MiSeq platform. Associations between bacterial taxa and clinical phenotypes were evaluated using multivariate association with
linear models statistical method. Blood levels of interleukin (IL)−1β, IL-6, tumour necrosis factor-α and adipokines were assessed by ELISA;
indoleamine 2,3-dioxygenase (IDO) activity was determined by high-performance liquid chromatography. Results Compared with healthy subjects, specific changes in faecal
microbiota were observed in FMF and AAA groups. Several operational taxonomic units (OTUs) were associated with FMF. Moreover, two OTUs were
over-represented in FMF-related AAA compared with FMF without AAA. Additionally, higher adiponectin levels and IDO activity were observed
in FMF-related AAA compared with FMF without AAA (p 〈 0.05). Conclusion The presence of specific changes in faecal microbiota in FMF and in
FMF-related AAA suggests that intestinal microorganisms may play a role in the pathogenesis of these diseases. These findings may offer an
opportunity to use techniques for gut microbiota manipulation.
Type of Medium:
Online Resource
ISSN:
0003-4967
,
1468-2060
DOI:
10.1136/annrheumdis-2019-215258
Language:
English
Publisher:
BMJ
Publication Date:
2019
detail.hit.zdb_id:
1481557-6
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