In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 3 ( 2023-3-16), p. e0281446-
Kurzfassung:
Blast-induced lung injury is associated with inflammatory, which are characterised by disruption of the alveolar-capillary barrier, haemorrhage, pulmonary infiltrateration causing oedema formation, pro-inflammatory cytokine and chemokine release, and anti-inflammatory counter-regulation. The objective of the current study was to define sequence of such alterations in with establishing blast-induced lung injury in rats using an advanced blast generator. Methods Rats underwent a standardized blast wave trauma and were euthanised at defined time points. Non-traumatised animals served as sham controls. Obtained samples from bronchoalveolar lavage fluid (BALF) at each time-point were assessed for histology, leukocyte infiltration and cytokine/chemokine profile. Results After blast lung injury, significant haemorrhage and neutrophil infiltration were observed. Similarly, protein accumulation, lactate dehydrogenase activity (LDH), alveolar eicosanoid release, matrix metalloproteinase (MMP)-2 and -9, pro-Inflammatory cytokines, including tumour necrosis factor (TNF) and interleukin (IL) -6 raised up. While declining in the level of anti-inflammatory cytokine IL-10 occurred. Ultimately, pulmonary oedema developed that increased to its maximum level within the first 1.5 h, then recovered within 24 h. Conclusion Using a stablished model, can facilitate the study of inflammatory response to blast lung injury. Following the blast injury, alteration in cytokine/chemokine profile and activity of cells in the alveolar space occurs, which eventuates in alveolar epithelial barrier dysfunction and oedema formation. Most of these parameters exhibit time-dependent return to their basal status that is an indication to resilience of lungs to blast-induced lung injury.
Materialart:
Online-Ressource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0281446
DOI:
10.1371/journal.pone.0281446.g001
DOI:
10.1371/journal.pone.0281446.g002
DOI:
10.1371/journal.pone.0281446.g003
DOI:
10.1371/journal.pone.0281446.g004
DOI:
10.1371/journal.pone.0281446.g005
DOI:
10.1371/journal.pone.0281446.g006
DOI:
10.1371/journal.pone.0281446.g007
DOI:
10.1371/journal.pone.0281446.g008
DOI:
10.1371/journal.pone.0281446.g009
DOI:
10.1371/journal.pone.0281446.g010
DOI:
10.1371/journal.pone.0281446.g011
DOI:
10.1371/journal.pone.0281446.g012
DOI:
10.1371/journal.pone.0281446.g013
DOI:
10.1371/journal.pone.0281446.t001
DOI:
10.1371/journal.pone.0281446.s001
DOI:
10.1371/journal.pone.0281446.s002
DOI:
10.1371/journal.pone.0281446.s003
DOI:
10.1371/journal.pone.0281446.s004
DOI:
10.1371/journal.pone.0281446.r001
DOI:
10.1371/journal.pone.0281446.r002
DOI:
10.1371/journal.pone.0281446.r003
DOI:
10.1371/journal.pone.0281446.r004
DOI:
10.1371/journal.pone.0281446.r005
DOI:
10.1371/journal.pone.0281446.r006
Sprache:
Englisch
Verlag:
Public Library of Science (PLoS)
Publikationsdatum:
2023
ZDB Id:
2267670-3
Bookmarklink