In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 5 ( 2022-5-26), p. e0269109-
Kurzfassung:
Lymph node culture-positive tuberculosis (LNTB+) is associated with increased mycobacterial antigen-induced pro-inflammatory cytokine production compared to LN culture-negative tuberculosis (LNTB-). However, the frequencies of CD4 + , CD8 + T cells and NK cells expressing Th1/Tc1/Type 1 (IFNγ, TNFα, IL-2), Th17/Tc17/Type 17 (IL-17A, IL-17F, IL-22) cytokines and cytotoxic (perforin [PFN], granzyme [GZE] B, CD107a) markers in LNTB+ and LNTB- individuals are not known. Thus, we have studied the unstimulated (UNS) and mycobacterial antigen-induced frequencies of CD4 + , CD8 + T and NK cells expressing Th1, Th17 cytokines and cytotoxic markers using flow cytometry. The frequencies of CD4 + , CD8 + T and NK cells expressing cytokines and cytotoxic markers were not significantly different between LNTB+ and LNTB- individuals in UNS condition. In contrast, upon Mtb antigen stimulation, LNTB+ individuals are associated with significantly increased frequencies of CD4 + T cells (PPD [IFNγ, TNFα], ESAT-6 PP [IFNγ, TNFα] , CFP-10 PP [IFNγ, TNFα, IL-2]), CD8 + T cells (PPD [IFNγ], ESAT-6 PP [IFNγ] , CFP-10 PP [TNFα]) and NK cells (PPD [IFNγ, TNFα] , ESAT-6 PP [IFNγ, TNFα], CFP-10 PP [TNFα] ) expressing Th1/Tc1/Type 1, but not Th17/Tc17/Type 17 cytokines and cytotoxic markers compared to LNTB- individuals. LNTB+ individuals did not show any significant alterations in the frequencies of CD4 + , CD8 + T cells and NK cells expressing cytokines and cytotoxic markers compared to LNTB- individuals upon HIV Gag PP and P/I antigen stimulation. Increased frequencies of CD4 + , CD8 + T and NK cells expressing Th1/Tc1/Type 1 cytokines among the LNTB+ group indicates that the presence of mycobacteria plays a dominant role in the activation of key correlates of immune protection or induces higher immunopathology.
Materialart:
Online-Ressource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0269109
DOI:
10.1371/journal.pone.0269109.g001
DOI:
10.1371/journal.pone.0269109.g002
DOI:
10.1371/journal.pone.0269109.g003
DOI:
10.1371/journal.pone.0269109.s001
DOI:
10.1371/journal.pone.0269109.s002
DOI:
10.1371/journal.pone.0269109.s003
DOI:
10.1371/journal.pone.0269109.s004
DOI:
10.1371/journal.pone.0269109.s005
DOI:
10.1371/journal.pone.0269109.s006
DOI:
10.1371/journal.pone.0269109.s007
DOI:
10.1371/journal.pone.0269109.s008
DOI:
10.1371/journal.pone.0269109.r001
DOI:
10.1371/journal.pone.0269109.r002
DOI:
10.1371/journal.pone.0269109.r003
DOI:
10.1371/journal.pone.0269109.r004
DOI:
10.1371/journal.pone.0269109.r005
DOI:
10.1371/journal.pone.0269109.r006
Sprache:
Englisch
Verlag:
Public Library of Science (PLoS)
Publikationsdatum:
2022
ZDB Id:
2267670-3
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