In:
The International Journal of Biological Markers, SAGE Publications, Vol. 29, No. 2 ( 2014-04), p. 180-183
Abstract:
The L1 cell adhesion molecule (L1CAM) is overexpressed in many human cancers and can serve as a biomarker for prognosis in most of these cancers (including type I endometrial carcinomas). Here we provide an optimized immunohistochemical staining procedure for a widely used automated platform (VENTANA™), which has recourse to commercially available primary antibody and detection reagents. In parallel, we optimized the staining on a semi-automated BioGenix (i6000) immunostainer. These protocols yield good stainings and should represent the basis for a reliable and standardized immunohistochemical detection of L1CAM in a variety of malignancies in different laboratories.
Type of Medium:
Online Resource
ISSN:
1724-6008
,
1724-6008
Language:
English
Publisher:
SAGE Publications
Publication Date:
2014
detail.hit.zdb_id:
1475778-3
Bookmarklink