Copyright information:Taken from "Experimental approaches to identify non-coding RNAs"Nucleic Acids Research 2006;34(2):635-646.Published online 25 Jan 2006PMCID:PMC1351373.© The Author 2006. Published by Oxford University Press. All rights reserved () Identification of ncRNAs by chemical or enzymatic sequencing of extracted abundant RNAs. () Identification of ncRNAs by cDNA cloning and sequencing; three different methods are indicated to reverse transcribe ncRNAs, usually lacking poly(A) tails, into cDNAs (e.g. by C-tailing, C-tailing and linker addition, or linker addition, only, followed by RT–RCR). () Identification of ncRNAs by micro-array analysis. DNA oligonucleotide covering the sequence space of an entire genome are spotted onto glass slides, to which fluorescently labelled samples, derived from cellular RNA, is hybridized. () Identification of ncRNAs by genomic SELEX. By random priming, the sequence of a genome is converted into short PCR fragments containing a T7 promotor at their 5′ ends. Subsequently, transcription by means of T7 RNA polymerase converts this genomic sequence of an organism into RNA fragments, which can then be assayed for function, such as binding to a specific protein or small chemical ligand, by SELEX....
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