Transcription start sites confirmed by 5′RACE. dRNA-Seq data are represented as number of read starts per base and gRNA-Seq data as read coverage, both are visualised using the Integrated Genome Browser . dRNA-Seq data are represented as read starts per base, gRNA-Seq data are represented as coverage. Blue arrows depict representative sequences derived from 5′RACE analysis. Panel A: pTSS of BCAL3153, confirmed by 2 out of 8 RACE sequences, 6 sequences were shorter than the putative 5′UTR. Panel B: pTSS of BCAL0672, confirmed by 5 out of 12 RACE sequences, 7 sequences were shorter than the putative 5′UTR. The isTSS internal to BCAL0672 was not confirmed by 5′RACE, transcripts originating from this locus are probably truncated. Panel C: pTSS of BCAL0301 confirmed by 2 out of 7 RACE sequences, 5 sequences were shorter than the putative 5′UTR. The isTSS located internal to BCAL0300 could not be confirmed, transcripts originating from this locus are probably truncated. Panel D: Internal pTSS of BCAL0063. 4 out of 5 RACE sequences confirmed the internal pTSS, one read was shorter. Panel E: isTSS within BCAL3201: 3 out of 9 RACE sequences confirmed the isTSS, 3 were shorter and 3 longer than the transcript originating at...
Biochemistry ; Microbiology ; Cell Biology ; Genetics ; Molecular Biology ; Neuroscience ; Biotechnology ; Evolutionary Biology ; Chemical Sciences Not Elsewhere Classified ; Ecology ; Immunology ; Cancer ; Mental Health ; Infectious Diseases ; Plant Biology
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