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  • 1
    In: Journal of Bacteriology, March, 1997, Vol.179(5-6), p.1764(10)
    Description: The nucleotide sequence of the gene encoding the major outer membrane protein (MOMP) of Haemophilus ducreyi was analyzed by sodium dodecyl sulfate-polyacrilamide gel electrophoresis. Nucleotide sequence analysis of the MOMP gene of Haemophilus ducreyi indicated the presence of two OmpA homologs that were encoded by momp and ompA2 genes. Southern blot analysis also indicated the high degree of similarity between MOMP and OmpA2 which existed in tandem in the different strains of Haemophilus ducreyi.
    Keywords: Pathogenic Bacteria -- Genetic Aspects ; Membrane Proteins -- Analysis ; Bacterial Proteins -- Analysis
    ISSN: 0021-9193
    E-ISSN: 10985530
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  • 2
    Language: English
    In: The Journal of Infectious Diseases, 1 March 2000, Vol.181(3), pp.1049-1054
    Description: Haemophilus ducreyi expresses a conserved hemoglobin-binding outer-membrane protein (HgbA). To test the role of HgbA in pathogenesis, we infected 9 adults with isolate 35000 and its isogenic hgbA-inactivated mutant (FX504) on their upper arms in a double-blinded, escalating dose-response study. Papules developed at similar rates at sites inoculated with the mutant or parent. The pustule-formation rate was 55% (95% confidence interval [CI], 30.8%-78.5%) at parent sites and 0 (95% CI, 0-10.5%) at mutant sites (P 〈 .0001). The recovery rate of H. ducreyi from surface cultures was 16% (n = 142) from parent sites and 0 (n = 213) from mutant sites (P 〈 .0001). H. ducreyi was recovered at biopsy from 6 of 7 parent sites and from 0 of 3 mutant sites. The results indicate that hemoglobin may be a critical source of heme or iron for the establishment of H. ducreyi infection in humans.
    Keywords: Physical sciences -- Chemistry -- Chemical compounds -- Haemophilus ducreyi ; Biological sciences -- Biology -- Microbiology -- Haemophilus ducreyi ; Biological sciences -- Biology -- Microbiology -- Haemophilus ducreyi ; Health sciences -- Medical conditions -- Infections -- Haemophilus ducreyi ; Physical sciences -- Chemistry -- Chemical compounds -- Haemophilus ducreyi ; Health sciences -- Medical diagnosis -- Diagnostic methods -- Haemophilus ducreyi ; Health sciences -- Medical sciences -- Pharmacology -- Haemophilus ducreyi ; Health sciences -- Medical sciences -- Immunology -- Haemophilus ducreyi ; Health sciences -- Medical conditions -- Physical trauma -- Haemophilus ducreyi ; Health sciences -- Health and wellness -- Public health -- Haemophilus ducreyi
    ISSN: 00221899
    E-ISSN: 15376613
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