Plant Cell, Tissue and Organ Culture (PCTOC), 2018, Vol.133(1), pp.51-61
To access, purchase, authenticate, or subscribe to the full-text of this article, please visit this link: http://dx.doi.org/10.1007/s11240-017-1360-y Byline: Mina Esfandiari (1,2), Mohsen Sharifi (1), Farzaneh Mohamadyar-Toupkanlou (3), Hana Hanaee-Ahwaz (4), Morteza Yousefzadi (5), Azizollah Jafari (6), Simzar Hosseinzadeh (7), Masoud Soleimani (8) Keywords: Linum persicum; Lignan; Podophyllotoxin; Anti-cancer; MCF7 cell line Abstract: Abstract Linum persicum contains significant amount of lignans as podophyllotoxin that may show therapeutic effects against several types of metastatic cancers such as breast cancer. The aim of this study was to establish the cell culture of L. persicum for lignans production and evaluation for anti-carcinogenic activity on MCF7 cell line. Also, lignan contents of the cell, callus, and plantlets and also in different parts including capsule, leave, stem and root of the wild plants were determined using HPLC technique. The highest podophyllotoxin in capsules and pinoresinol in leaf and root organs were observed. Shoot extract of the in-vitro plantlets have greater cytotoxic effect on breast cancer cells (IC50=5 A[micro]g/ml) and leads to significant raise in ratio of Bax/Bcl-2 value=1.42 and Rbl1 gene expression that are well-known as apoptotic inducing agents. In conclusion, due to cytotoxic activity of in vitro plantlet extracts on MCF7 cell line with limited side-effects the corresponding extracted component could be led to therapeutic approaches in future. Graphical abstract Author Affiliation: (1) 0000 0001 1781 3962, grid.412266.5, Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, 14115-154, Iran (2) grid.419654.b, Department of Nanotechnology and Tissue Engineering, Stem Cell Technology Research Center, Tehran, Iran (3) 0000 0001 1781 3962, grid.412266.5, Department of Chemical Engineering, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran (4) grid.419654.b, Stem Cell Biology Department, Stem Cell Technology Research Center, Tehran, Iran (5) grid.444744.3, Department of Marine Biology, Hormozgan University, Bandar Abbas, Iran (6) grid.440825.f, Department of Phytochemistry, Faculty of Science, Yasouj University, Yasouj, Iran (7) grid.411600.2, Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran (8) 0000 0001 1781 3962, grid.412266.5, Department of Hematology, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran Article History: Registration Date: 23/11/2017 Received Date: 24/07/2017 Accepted Date: 22/11/2017 Online Date: 11/12/2017 Article note: Communicated by Sergio J. Ochatt. The original version of this article has been revised: The seventh author's first name has been corrected. A correction to this article is available online at https://doi.org/10.1007/s11240-018-1386-9.
Linum persicum ; Lignan ; Podophyllotoxin ; Anti-cancer ; MCF7 cell line
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