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  • Gene Expression
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  • 1
    Language: English
    In: Journal of the American College of Cardiology, 05 April 2011, Vol.57(14), pp.E1202-E1202
    Description: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/S0735-1097(11)61202-3 Byline: Mohsen Sharifi, Rima Patel, Tariq Niazi, Elnaz Emrani, Mahshid Mehdipour
    Keywords: Medicine
    ISSN: 0735-1097
    E-ISSN: 1558-3597
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  • 2
    Language: English
    In: Preparative Biochemistry and Biotechnology, 03 July 2016, Vol.46(5), pp.454-460
    Description: Feeding experiments with hairy root cultures of Linum album have established that the extracellular coniferaldehyde is a good precursor for production of two lignans: lariciresinol (LARI) and pinoresinol (PINO). The accumulation of the LARI, PINO, and podophyllotoxin (PTOX) in hairy roots were...
    Keywords: 3,4-(Methylenedioxy)Cinnamic Acid ; Coniferaldehyde ; Gene Expression ; Hairy Roots ; Lignan ; Linum Album ; Biology
    ISSN: 1082-6068
    E-ISSN: 1532-2297
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  • 3
    Language: English
    In: Journal of Plant Physiology, July 2018, Vol.226, pp.163-171
    Description: Iron is a component of many proteins that have crucial roles in plant growth and development, such as ferritin and catalase. Iron also, as a ferromagnetic element, is assumed to be influenced by a static magnetic field (SMF). In the present study, we examined the relationship between ferrous content and gene expression and activity of ferritin and catalase in soybean plants under the influence of 0, 20, and 30 mT SMF for 5 day, 5 h each. Exposure to 20 mT decreased gene expression of Fe transporter, ferrous and H O contents and gene expression, content and activity of ferritin and catalase. Opposite responses were observed under 30 mT treatments. The results suggest that SMF triggered a signaling pathway that is mediated by iron. The structure and activity of purified ferritin and apoferritin from horse spleen, and catalase from bovine liver proteins under SMF were evaluated as well. Secondary structure of proteins were not influenced by SMF (evidenced by far-UV circular dichroism), whereas their tertiary structure, size, and activity were altered (shown by fluorescence spectroscopy and dynamic light-scattering). From these results, it is likely that the number of iron atoms is involved in the nature of influence of SMF on protein structure.
    Keywords: Apoferritin ; Catalase ; Ferritin ; Ferrous ; Fe Transporter ; H2o2 ; Botany
    ISSN: 0176-1617
    E-ISSN: 16181328
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  • 4
    Language: English
    In: Environmental and Experimental Botany, September 2017, Vol.141, pp.41-49
    Description: The mechanisms through which nitric oxide (NO) can influence plant tolerance to Pb are unknown, but may be based on the accumulation of secondary metabolites such as phenolic compounds and polyamines (PAs). This hypothesis was tested by exposing hydroponically-cultured to Pb alone and in combination with sodium nitroprusside (SNP; NO donor), L-NAME (NO synthase inhibitor) and tungstate (TUN; nitrate reductase inhibitor) for a time course of 72 h. The inhibitors strongly repressed NO generation, verifying that Pb and Pb + SNP-induced NO is probably mediated by nitrite and arginine-dependent pathways. The results show that the Pb-induced inhibition of growth was alleviated through application of NO donor, which decreased Pb accumulation in the plant samples, but deteriorated with the decrease in NO, which indicates a higher level of Pb absorbed. The elevated contents of phenolic acids under Pb and Pb + SNP treatments was reduced by the NOS and NR inhibitors and was significantly associated with phenylalanine ammonia-lyase ( ) gene expression patterns. The arginine decarboxylase ( ) gene in the PAs biosynthesis pathway remained unchanged. Considerable alteration was observed in the conjugated PAs in response to the Pb and Pb + SNP applications when compared with the NR and NOS inhibitors that exhibited an increase in free PAs. The results suggest that Pb-evoked NO promotes homeostasis of metabolic pathways to phenolic acids and conjugated PAs that enhance lignification to strengthen against stress.
    Keywords: Nitric Oxide ; Phenolic Compounds ; Polyamines ; No Synthase ; Nitrate Reductase ; Prosopis Farcta ; Environmental Sciences ; Botany
    ISSN: 0098-8472
    E-ISSN: 1873-7307
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  • 5
    Language: English
    In: Planta, 2018, Vol.248(5), pp.1289-1306
    Description: To access, purchase, authenticate, or subscribe to the full-text of this article, please visit this link: http://dx.doi.org/10.1007/s00425-018-2973-z Byline: Hannaneh Tashackori (1), Mohsen Sharifi (1), Najmeh Ahmadian Chashmi (2), Mehrdad Behmanesh (3), Naser Safaie (4) Keywords: Elicitation; Gene expression; Hairy root; Lignan; Linum album; Phenylpropanoids Abstract: Main conclusion Elicitation of Linum album hairy roots by Piriformospora indica cell wall induced the target genes and specific metabolites in phenylpropanoid pathway and shifted the amino acid metabolism toward the phenolic compound production. Plants have evolved complex mechanisms to defend themselves against various biotic stresses. One of these responses is the production of metabolites that act as defense compounds. Manipulation of plant cell cultures by biotic elicitors is a useful strategy for improving the production of valuable secondary metabolites. This study focused on hairy root culture of Linum album, an important source for lignans. The effects of cell wall elicitor extracted from Piriformospora indica on phenylpropanoid derivatives were evaluated to identify metabolic traits related to biotic stress tolerance. Significant increases in lignin, lignans lariciresinol, podophyllotoxin, and 6-methoxy podophyllotoxin phenolic acids: cinnamic acid, ferulic acid, and salicylic acid flavonoids: myricetin, kaempferol, and diosmin were observed in response to the fungal elicitor. In addition, the gene expression levels of phenylalanine ammonia-lyase, cinnamyl alcohol dehydrogenase, cinnamoyl-CoA reductase, and pinoresinol--lariciresinol reductase significantly increased after elicitation. The composition of free amino acids was altered under the elicitation. Phenylalanine and tyrosine, as precursors of phenylpropanoid metabolites, were increased, but alanine, serine, and glutamic acid significantly decreased in response to the fungal elicitor, suggesting that the amino acid pathway may be shifted toward biosynthesis of aromatic amino acids and precursors of the phenylpropanoid pathway. These results provided evidence that up-regulation of genes involved in the phenylpropanoid pathway in response to the fungal elicitor resulted in enhanced metabolic responses associated with the protection in L. album. This approach can also be applied to improve lignan production. Author Affiliation: (1) 0000 0001 1781 3962, grid.412266.5, Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, 14115-154, Tehran, Iran (2) 0000 0000 9618 7703, grid.411622.2, Department of Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran (3) 0000 0001 1781 3962, grid.412266.5, Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran (4) 0000 0001 1781 3962, grid.412266.5, Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran Article History: Registration Date: 06/08/2018 Received Date: 14/03/2018 Accepted Date: 04/08/2018 Online Date: 14/08/2018
    Keywords: Elicitation ; Gene expression ; Hairy root ; Lignan ; Linum album ; Phenylpropanoids
    ISSN: 0032-0935
    E-ISSN: 1432-2048
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  • 6
    Language: English
    In: Planta, 2017, Vol.245(6), pp.1165-1178
    Description: To access, purchase, authenticate, or subscribe to the full-text of this article, please visit this link: http://dx.doi.org/10.1007/s00425-017-2671-2 Byline: Neda Javadian (1), Ghasem Karimzadeh (1), Mohsen Sharifi (2), Ahmad Moieni (1), Mehrdad Behmanesh (3) Keywords: Linum album; Podophyllotoxin; Tetraploidy; Gene expression; Flow cytometry Abstract: Main conclusion Induction of tetraploidy was performed and podophyllotoxin production increased by upregulating the expression level and enzyme activity of genes related to its biosynthesis in tetraploid compared to diploid Linum album. Linum album is a valuable medicinal plant that produces antiviral and anticancer compounds including podophyllotoxin (PTOX). To achieve homogeneous materials, in vitro diploid clones were established, and their nodal segments were exposed to different concentrations and durations of colchicine. This resulted in successful tetraploidy induction, confirmed by flow cytometry, and is being reported for the first time. The highest efficiency of tetraploid induction (22%) was achieved after 72 h exposure to 2.5-mM colchicine treatment. The stable tetraploids were produced after being subcultured three times, and their ploidy stability was confirmed after each subculture. The effects of autopolyploidy were measured on the morphological and phytochemical characteristics, as well as enzyme activity and the expression levels of some key genes involved in the PTOX biosynthetic pathway, including phenylalanine ammonia-lyase (PAL), cinnamoyl-Coa reductase (CCR), cinnamyl-alcohol dehydrogenase (CAD), and pinoresinol-lariciresinol reductase (PLR). The tetraploid plants had larger leaves and stomata (length and width) and lower density stomata. Increasing the ploidy level from diploid to tetraploid resulted in 1.39- and 1.23-fold enhancement of PTOX production, respectively, in the leaves and stem. The increase in PTOX content was associated with upregulated activities of some enzymes studied related to its biosynthetic pathway and the expression of the corresponding genes. The expression of the PAL gene and PLR enzymatic activity had the most positive correlation with the ploidy level in both leaf and stem tissues. Our results verified that autotetraploid induction is a useful breeding method, remarkably increasing the PTOX content in the leaves and stem of L. album. Author Affiliation: (1) Department of Plant Breeding, Faculty of Agriculture, Tarbiat Modares University, P. O. Box 14115-336, Tehran, Iran (2) Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran (3) Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran Article History: Registration Date: 06/03/2017 Received Date: 15/12/2016 Accepted Date: 04/03/2017 Online Date: 14/03/2017 Article note: Electronic supplementary material The online version of this article (doi: 10.1007/s00425-017-2671-2) contains supplementary material, which is available to authorized users.
    Keywords: Linum album ; Podophyllotoxin ; Tetraploidy ; Gene expression ; Flow cytometry
    ISSN: 0032-0935
    E-ISSN: 1432-2048
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  • 7
    Language: English
    In: Plant Physiology and Biochemistry, October 2016, Vol.107, pp.374-384
    Description: Manipulation of cell culture media by elicitors is one of most important strategies to inducing secondary metabolism for the production of valuable metabolites. In this investigation, inducing effect of chitosan on physiological, biochemical, and molecular parameters were investigated in cell suspension cultures of Boiss. The results showed that chitosan concentration and time of elicitation are determinants of the effectiveness of the elicitor. Accumulation of aromatic amino acids (phenylalanine [Phe] and tyrosine [Tyr]), phenylpropanoid compounds (phenolic acids [PAs] and echinacoside [ECH]), hydrogen peroxide (H O ) production, phenylalanine ammonia-lyase (PAL) activity and gene expression, and antioxidant enzymes (superoxide dismutase [SOD], peroxidase [POX], catalase [CAT]) activities were altered by changing the exposure time of elicitation. Results showed that, upon elicitation with chitosan, oxidative events were induced, antioxidant responses of . cells were boosted through enhanced activity of an effective series of scavenging enzymes (SOD, CAT, and POX), and biosynthesis of non-enzymatic antioxidants (ECH and PAs [cinnamic, -coumaric and, caffeic acids]). The increase in amino acid content and PAL activity at early days of exposure to chitosan was related with rises in phenolic compounds. These results provide evidence that chitosan by up-regulation of gene differentially improves the production of phenylpropanoid compounds, which are of medical commercial value with good biotechnological prospects.
    Keywords: Aromatic Amino Acids ; Antioxidant Enzymes ; Chitosan ; Phenylalanine Ammonia-Lyase ; Phenylpropanoid Compounds ; Plant Cell Culture ; Scrophularia Striata Boiss ; Botany ; Chemistry
    ISSN: 0981-9428
    E-ISSN: 1873-2690
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  • 8
    Language: English
    In: Cell Journal, 01 October 2016, Vol.17(3), pp.502-509
    Description: Objective Podophyllotoxin (PTOX), a natural compound in numerous plants, contains remarkable biological properties that include anti-tumor, anti-viral such as anti-human im- munodeficiency virus (HIV) activities. In order to avoid its adverse effects, various com- pounds have been derived from PTOX. 6-methoxy PTOX (MPTOX) is one of the natural PTOX derivatives with an extra methoxy group. MPTOX is mostly isolated from the Linum species. This study has sought to determine the biological effects of MPTOX on cancer cell lines, 5637 and K562. Materials and Methods In this experimental study, we treated the 5637 and K562 cancer cell lines with MPTOX in a doseand time-dependent manner. Apoptosis was examined by flow cytometry and viability rate was analyzed by the MTT assay. Expressions of the tubulin (TUBB3) and topoisomerase II (TOPIIA) genes were determined by real-time poly- merase chain reaction (PCR). Results Treatment with MPTOX led to significant induction of apoptosis in cancer cells compared to control cells. Gene expression analysis showed reduced levels of TUBB3 and TOPIIA mRNA following MPTOX treatment. Conclusion MPTOX inhibited TUBB3 and TOPIIA gene expression and subsequently induced cell death through apoptosis. These results suggested that MPTOX could be considered a potential anti-tumor agent.
    Keywords: Podophyllotoxin ; Cancer ; Apoptosis ; Gene Expression ; Real-Time Pcr ; Anatomy & Physiology
    ISSN: 2228-5806
    E-ISSN: 2228-5814
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  • 9
    Language: English
    In: Plant Cell, Tissue and Organ Culture (PCTOC), 2018, Vol.133(1), pp.51-61
    Description: To access, purchase, authenticate, or subscribe to the full-text of this article, please visit this link: http://dx.doi.org/10.1007/s11240-017-1360-y Byline: Mina Esfandiari (1,2), Mohsen Sharifi (1), Farzaneh Mohamadyar-Toupkanlou (3), Hana Hanaee-Ahwaz (4), Morteza Yousefzadi (5), Azizollah Jafari (6), Simzar Hosseinzadeh (7), Masoud Soleimani (8) Keywords: Linum persicum; Lignan; Podophyllotoxin; Anti-cancer; MCF7 cell line Abstract: Abstract Linum persicum contains significant amount of lignans as podophyllotoxin that may show therapeutic effects against several types of metastatic cancers such as breast cancer. The aim of this study was to establish the cell culture of L. persicum for lignans production and evaluation for anti-carcinogenic activity on MCF7 cell line. Also, lignan contents of the cell, callus, and plantlets and also in different parts including capsule, leave, stem and root of the wild plants were determined using HPLC technique. The highest podophyllotoxin in capsules and pinoresinol in leaf and root organs were observed. Shoot extract of the in-vitro plantlets have greater cytotoxic effect on breast cancer cells (IC50=5 A[micro]g/ml) and leads to significant raise in ratio of Bax/Bcl-2 value=1.42 and Rbl1 gene expression that are well-known as apoptotic inducing agents. In conclusion, due to cytotoxic activity of in vitro plantlet extracts on MCF7 cell line with limited side-effects the corresponding extracted component could be led to therapeutic approaches in future. Graphical abstract Author Affiliation: (1) 0000 0001 1781 3962, grid.412266.5, Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, 14115-154, Iran (2) grid.419654.b, Department of Nanotechnology and Tissue Engineering, Stem Cell Technology Research Center, Tehran, Iran (3) 0000 0001 1781 3962, grid.412266.5, Department of Chemical Engineering, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran (4) grid.419654.b, Stem Cell Biology Department, Stem Cell Technology Research Center, Tehran, Iran (5) grid.444744.3, Department of Marine Biology, Hormozgan University, Bandar Abbas, Iran (6) grid.440825.f, Department of Phytochemistry, Faculty of Science, Yasouj University, Yasouj, Iran (7) grid.411600.2, Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran (8) 0000 0001 1781 3962, grid.412266.5, Department of Hematology, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran Article History: Registration Date: 23/11/2017 Received Date: 24/07/2017 Accepted Date: 22/11/2017 Online Date: 11/12/2017 Article note: Communicated by Sergio J. Ochatt. The original version of this article has been revised: The seventh author's first name has been corrected. A correction to this article is available online at https://doi.org/10.1007/s11240-018-1386-9.
    Keywords: Linum persicum ; Lignan ; Podophyllotoxin ; Anti-cancer ; MCF7 cell line
    ISSN: 0167-6857
    E-ISSN: 1573-5044
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  • 10
    Language: English
    In: Industrial Crops & Products, July 2019, Vol.133, pp.105-110
    Description: The potential for product recovery of metabolites can be improved by several methods; one of the most common techniques is elicitation. Elicitors can adjust multiple control points and precipitate the expression of some key genes. This project was conducted with the aim of increasing valuable secondary metabolites such as D-9-tetrahydrocannabinol (THC) and cannabidiol (CBD) identified in cannabis plants. Whole plants were treated with salicylic acid (SA) and γ-Aminobutyric acid (GABA) to evaluate their effect on , , and genes which are responsible for production of the main cannabinoids. THC and CBD contents in related samples were measured using HPLC, 72 h after treatments. expression was enhanced and reached its maximum level with 1 mM salicylic acid; however, the expression of , and decreased. Furthermore, treatment with GABA enhanced the expression mainly at 0.1 mM. The highest THC content was obtained in 1 mM salicylic acid and 0.1 mM GABA treatments, respectively. The results suggested that salicylic acid and GABA can control the signaling cascades of genes in cannabinoid pathway by changing their expression patterns at critical concentration, and these two compounds can be considered as effective elicitors for commercial cannabinoid production. Designing an appropriate standard protocol based on spraying approach on plants to elicit specific metabolites such as THC and CBD which are mainly produced at flowering time and in flowering organs has many benefits alongside other elicitation approaches.
    Keywords: Cannabis Sativa ; Elicitors ; Gene Expression ; Gaba ; Thc ; Cbd ; Agriculture
    ISSN: 0926-6690
    E-ISSN: 1872-633X
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