Kooperativer Bibliotheksverbund

Berlin Brandenburg

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  • 1
    Language: English
    In: The Journal of Infectious Diseases, 1 March 2000, Vol.181(3), pp.1049-1054
    Description: Haemophilus ducreyi expresses a conserved hemoglobin-binding outer-membrane protein (HgbA). To test the role of HgbA in pathogenesis, we infected 9 adults with isolate 35000 and its isogenic hgbA-inactivated mutant (FX504) on their upper arms in a double-blinded, escalating dose-response study. Papules developed at similar rates at sites inoculated with the mutant or parent. The pustule-formation rate was 55% (95% confidence interval [CI], 30.8%-78.5%) at parent sites and 0 (95% CI, 0-10.5%) at mutant sites (P 〈 .0001). The recovery rate of H. ducreyi from surface cultures was 16% (n = 142) from parent sites and 0 (n = 213) from mutant sites (P 〈 .0001). H. ducreyi was recovered at biopsy from 6 of 7 parent sites and from 0 of 3 mutant sites. The results indicate that hemoglobin may be a critical source of heme or iron for the establishment of H. ducreyi infection in humans.
    Keywords: Physical sciences -- Chemistry -- Chemical compounds -- Haemophilus ducreyi ; Biological sciences -- Biology -- Microbiology -- Haemophilus ducreyi ; Biological sciences -- Biology -- Microbiology -- Haemophilus ducreyi ; Health sciences -- Medical conditions -- Infections -- Haemophilus ducreyi ; Physical sciences -- Chemistry -- Chemical compounds -- Haemophilus ducreyi ; Health sciences -- Medical diagnosis -- Diagnostic methods -- Haemophilus ducreyi ; Health sciences -- Medical sciences -- Pharmacology -- Haemophilus ducreyi ; Health sciences -- Medical sciences -- Immunology -- Haemophilus ducreyi ; Health sciences -- Medical conditions -- Physical trauma -- Haemophilus ducreyi ; Health sciences -- Health and wellness -- Public health -- Haemophilus ducreyi
    ISSN: 00221899
    E-ISSN: 15376613
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  • 2
    Language: English
    In: The Journal of Infectious Diseases, 1 July 1986, Vol.154(1), pp.100-109
    Description: Eighty-six nasopharyngeal isolates of Haemophilus influenzae were prospectively obtained from three children who attended a day care center from infancy until early childhood (five to seven years). A majority of the strains were nontypable. We analyzed strains by comparing their biotypes and by performing electrophoresis of outer membrane proteins on polyacrylamide gels. Profiles of outer membrane proteins were very heterogeneous and could not be used as the basis for the development of a subtyping scheme. The children characteristically carried a nasopharyngeal strain defined by a unique outer membrane pattern for a period of months, lost it, and then acquired a new strain. We probed the outer membrane proteins of a child's strains by the western blot technique with serum obtained serially from the child. Isolates whose outer membrane proteins appeared identical on stained gels generally had similar antigenic bands on western blots but were occasionally immunologically distinct. Serum immunoglobulins of the IgG class that reacted with the outer membrane proteins did not appear to change greatly over time or to play a role in preventing or terminating colonization. We conclude that nasopharyngeal colonization in children by nontypable H. influenzae is a dynamic process and that factors that cause loss and acquisition of strains remain to be determined
    Keywords: Biological sciences -- Biology -- Microbiology ; Health sciences -- Medical sciences -- Immunology ; Applied sciences -- Materials science -- Materials ; Health sciences -- Medical sciences -- Immunology ; Behavioral sciences -- Sociology -- Human societies ; Health sciences -- Medical conditions -- Infections ; Health sciences -- Health and wellness -- Public health ; Health sciences -- Medical conditions -- Infections ; Physical sciences -- Chemistry -- Chemical compounds ; Health sciences -- Medical conditions -- Diseases
    ISSN: 00221899
    E-ISSN: 15376613
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  • 3
    Language: English
    In: The Journal of Infectious Diseases, 1 May 1994, Vol.169(5), pp.1146-1150
    Description: Four subjects were experimentally infected with Haemophilus ducreyi. Lesions developed only at sites where live bacteria were inoculated on abraded skin. No subject developed fever, lymphadenopathy, or disseminated infection during a 3-day observation period. Two subjects who were rechallenged 2 months after initial infection also developed lesions. The amount of H. ducreyi recovered from 10 of 12 biopsies that were semiquantitatively cultured varied widely. Similar histologic features were present in initial and second infections. The epidermis contained pustules; the dermis contained an infiltrate of T cells and macrophages and reactive endothelial cells. Keratinocytes and T cells expressed HLA-DR, consistent with a delayed-type hypersensitivity response. The subjects did not mount humoral responses to bacterial proteins and to lipooligosaccharides after primary and secondary challenges. Thus, human experimental infection with H. ducreyi is well tolerated and safe. Recruitment of T cells and macrophages into chancroid lesions may partially explain the association between chancroid and human immunodeficiency virus transmission.
    Keywords: Biological sciences -- Biology -- Physiology -- Heterophils ; Biological sciences -- Biology -- Physiology -- Heterophils ; Health sciences -- Medical conditions -- Infections -- Heterophils ; Biological sciences -- Biology -- Physiology -- Heterophils ; Biological sciences -- Biochemistry -- Biomolecules -- Heterophils ; Biological sciences -- Biochemistry -- Biomolecules -- Heterophils ; Health sciences -- Medical conditions -- Diseases -- Heterophils ; Health sciences -- Medical conditions -- Infections -- Heterophils ; Biological sciences -- Biochemistry -- Biomolecules -- Heterophils ; Health sciences -- Medical conditions -- Diseases -- Heterophils
    ISSN: 00221899
    E-ISSN: 15376613
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  • 4
    Language: English
    In: The Journal of Infectious Diseases, 1 June 1998, Vol.177(6), pp.1608-1613
    Description: Human subjects were infected with Haemophilus ducreyi. All subjects developed papules and were randomized to treatment with a single dose of azithromycin (1 g) or ciprofloxacin (500 mg). At weekly intervals, volunteers were reinoculated with H. ducreyi, and drug concentrations were measured in peripheral blood mononuclear cells (PBMC). When papules developed, the subjects were treated with antibiotics and dismissed from the study. Eight of the ciprofloxacin-treated subjects developed papules 1 week after the initial treatment, and the ninth subject developed disease 2 weeks after treatment. The 9 azithromycin-treated subjects developed papules 4-10 weeks (mean, 6.8) after the initial treatment (P 〈 .001). Azithromycin was detected in PBMC for 3-6 weeks (mean, 4). Pre-and posttreatment lesions had histology typical of experimental chancroid or were culture positive. Azithromycin prevents experimental chancroid for nearly 2 months. These findings have implications for strategies to prevent chancroid.
    Keywords: Health sciences -- Medical conditions -- Infections -- Haemophilus ducreyi ; Health sciences -- Medical sciences -- Pharmacology -- HIV ; Health sciences -- Medical conditions -- Infections -- HIV ; Health sciences -- Medical diagnosis -- Diagnostic methods -- HIV ; Biological sciences -- Biology -- Microbiology -- HIV ; Biological sciences -- Biology -- Microbiology -- HIV ; Biological sciences -- Biology -- Microbiology -- HIV ; Health sciences -- Medical specialties -- Pathology -- HIV ; Health sciences -- Medical conditions -- Physical trauma -- HIV ; Physical sciences -- Physics -- Microphysics -- HIV
    ISSN: 00221899
    E-ISSN: 15376613
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  • 5
    Language: English
    In: The Journal of Infectious Diseases, 1 March 2000, Vol.181(3), pp.1176-1179
    Description: Haemophilus ducreyi expresses fine tangled pili, which are composed predominantly of a major subunit (FtpA). Confocal microscopy showed that an FtpA-specific monoclonal antibody bound to bacteria in biopsy samples obtained from infected human volunteers. To test the role of pili in pathogenesis, an isogenic mutant (35000HP-SMS1) was constructed by insertionally inactivating ftpA 35000HP-SMS1 did not express FtpA and was nonpiliated but was otherwise identical to its parent, 35000HP. Seven healthy adults were challenged on the upper arm with the isogenic isolates in a double-blinded, escalating dose-response study. Sites inoculated with the mutant produced papules and pustules at rates similar to the rates observed at sites inoculated with the parent. The recovery rate of H. ducreyi from cultures and the histopathology of biopsy samples obtained from pustules inoculated with 35000HP or 35000HP-SMS1 were similar. Although pili are expressed in vivo, FtpA is not required for pustule formation in the human challenge model.
    Keywords: Biological sciences -- Biology -- Microbiology -- Haemophilus ducreyi ; Health sciences -- Medical diagnosis -- Diagnostic methods -- Polyclonal antibodies ; Biological sciences -- Biology -- Microbiology -- Polyclonal antibodies ; Health sciences -- Medical conditions -- Physical trauma -- Polyclonal antibodies ; Health sciences -- Medical conditions -- Infections -- Polyclonal antibodies ; Health sciences -- Medical sciences -- Immunology -- Polyclonal antibodies ; Health sciences -- Medical sciences -- Immunology -- Polyclonal antibodies ; Applied sciences -- Laboratory techniques -- Microscopy -- Polyclonal antibodies ; Health sciences -- Medical sciences -- Immunology -- Polyclonal antibodies ; Physical sciences -- Chemistry -- Chemical compounds -- Polyclonal antibodies
    ISSN: 00221899
    E-ISSN: 15376613
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