Journal of Biophotonics, September 2019, Vol.12(9), pp.n/a-n/a
, also known as Group B (GBS), is a major cause of chorioamnionitis and neonatal sepsis. This study evaluates Raman spectroscopy (RS) to identify spectral characteristics of infection and differentiate GBS from and during ex vivo infection of human fetal membrane tissues. Unique spectral features were identified from colonies grown on agar and infected fetal membrane tissues. Multinomial logistic regression analysis accurately identified GBS infected tissues with 100.0% sensitivity and 88.9% specificity. Together, these findings support further investigation into the use of RS as an emerging microbiologic diagnostic tool and intrapartum screening test for GBS carriage. Current methods to screen Group B (GBS), a major cause of chorioamnionitis and neonatal sepsis, do not provide accurate, sensitive readings. Raman microspectroscopy combined with logistic regression was utilized to investigate a GBS infection model of human fetal membranes ex vivo. Tissue infected with GBS was successfully distinguished from non‐infected, infected, and infected tissue. These findings motivate the development of Raman spectroscopy as a diagnostic tool for intrapartum screening of GBS.
Biofilms ; Chorioamnionitis ; Gbs ; Group B Streptococcus ; Raman Spectroscopy ; Streptococcus Agalactiae