In:
Journal of Phytopathology, Wiley, Vol. 162, No. 3 ( 2014-03), p. 180-189
Abstract:
Five asparagus cultivars, three breeding lines and the wild relative Asparagus amarus were tested for natural infection by Asparagus virus 1 ( AV ‐1) in experimental fields at two locations over 3 and 4 years, respectively. In the first year after re‐planting the annual crowns in the field, more than 90% of tested plants of cultivars were infected by AV ‐1. In the third and fourth year, 100% of tested plants of cultivars were AV ‐1 infected. In comparison, all plants of the wild relative A. amarus were completely free of AV ‐1, suggesting a high level of resistance. Additionally, 1‐year‐old glasshouse‐cultivated plants of A. officinalis and A. amarus were placed in an AV ‐1 provocation cabin under field conditions. Seven months later, 100% of the A. officinalis plants showed a high virus concentration in ELISA , whereas no AV ‐1 was detectable in the A. amarus plants. This result was confirmed by highly sensitive AV ‐1‐specific RT ‐ PCR . To exclude vector resistance, the feeding behaviour of green peach aphid Myzus persicae was tested over 12 h using the electrical penetration graph method. Both asparagus genotypes were accepted by the aphids as potential hosts, but the feeding time was significantly longer on A. amarus . A genetic distance analysis of the various cultivars of Asparagus officinalis and selected wild relatives of the JKI collection was carried out, resulting in a clear discrimination of cultivars and wild relatives, especially A. amarus . The potential breeding value of the putative resistance carrier is discussed.
Type of Medium:
Online Resource
ISSN:
0931-1785
,
1439-0434
DOI:
10.1111/jph.2014.162.issue-3
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2020539-9
SSG:
12
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