In:
The International Journal of Biological Markers, SAGE Publications, Vol. 4, No. 4 ( 1989-10), p. 221-225
Abstract:
Expression of 40 kDa 2'-5' oligo (A) synthetase can serve as a marker of interferon (IFN) activity on the biological target. The mechanisms of induction of human 40 kDa 2'-5' oligo (A) synthetase by IFNs were investigated in HeLa and Molt 4 cells. Using a combined treatment with cycloheximide and actinomycin D we observed that in HeLa cells IFN-α did not need ongoing protein synthesis to induce the enzyme, whereas the addition of cycloheximide prevented the induction by IFN-γ. IFN-α induced the 40 kD enzyme in the T-cell line Molt 4 to a level comparable to that in HeLa cells, but only in the presence of active protein synthesis. These results suggest that an early response gene coding for a positive IFN-inducible protein may be needed in T cells, but not in HeLa cells to regulate the transcription of this 2'-5' oligo (A) synthetase gene.
Type of Medium:
Online Resource
ISSN:
1724-6008
,
1724-6008
DOI:
10.1177/172460088900400407
Language:
English
Publisher:
SAGE Publications
Publication Date:
1989
detail.hit.zdb_id:
1475778-3
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