In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 9 ( 2021-9-23), p. e0256106-
Abstract:
Indiscriminate use of antibiotics to treat infections that are of viral origin contributes to unnecessary use which potentially may induce resistance in commensal bacteria. To counteract this a number of host gene transcriptional studies have been conducted to identify genes that are differently expressed during bacterial and viral infections in humans, and thus could be used as a tool to base decisions on the use of antibiotics. In this paper, we aimed to evaluate the potential of a selection of genes that have been considered biomarkers in humans, to differentially diagnose bacterial from viral infections in the pig. First porcine PBMC were induced with six toll-like receptor (TLR) agonists (FliC, LPS, ODN 2216, Pam3CSK4, poly I:C, R848) to mimic host gene expression induced by bacterial or viral pathogens, or exposed to heat-killed Actinobacillus pleuropneumoniae or a split influenza virus. Genes that were differentially expressed between bacterial and viral inducers were further evaluated on clinical material comprising eleven healthy pigs, and six pigs infected with A . pleuropneumoniae . This comprised three virally upregulated genes ( IFI44L , MxA , RSAD2 ) and four bacterially upregulated genes (IL-1β, IL-8 , FAM89A , S100PBP ). All six infected pigs could be differentially diagnosed to healthy pigs using a host gene transcription assay based on the geometric average of the bacterially induced genes IL-8 and S100PBP over that of the virally induced gene MxA .
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0256106
DOI:
10.1371/journal.pone.0256106.g001
DOI:
10.1371/journal.pone.0256106.g002
DOI:
10.1371/journal.pone.0256106.g003
DOI:
10.1371/journal.pone.0256106.g004
DOI:
10.1371/journal.pone.0256106.t001
DOI:
10.1371/journal.pone.0256106.t002
DOI:
10.1371/journal.pone.0256106.s001
DOI:
10.1371/journal.pone.0256106.s002
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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