Format:
Online-Ressource
ISSN:
1439-7633
Content:
Abstract: The mechanism of action of aurein 2.2 and aurein 2.3, antimicrobial peptides from the frog Litoria aurea, was investigated. Proteomic profiling of the Bacillus subtilis stress response indicates that the cell envelope is the main target for both aureins. Upon treatment, the cytoplasmic membrane depolarizes and cellular ATP levels decrease. Global element analysis shows that intracellular concentrations of certain metal ions (potassium, magnesium, iron, and manganese) strongly decrease. Selective translocation of some ions over others was demonstrated in vitro. The same set of ions also leaks from B. subtilis cells treated with sublethal concentrations of gramicidin S, MP196, and nisin. Aureins do not permeabilize the cell membrane for propidium iodide thus excluding formation of large, unspecific pores. Our data suggest that the aureins acts by forming small pores thereby causing membrane depolarization, and by triggering the release of certain metal ions thus disturbing cellular ion homeostasis.
In:
volume:16
In:
number:7
In:
year:2015
In:
pages:1101-1108
In:
extent:8
In:
ChemBioChem, Weinheim : Wiley-VCH, [2000]-, 16, Heft 7 (2015), 1101-1108 (gesamt 8), 1439-7633
Language:
English
DOI:
10.1002/cbic.201500020
URN:
urn:nbn:de:101:1-2022122406314756438817
URL:
https://doi.org/10.1002/cbic.201500020
URL:
https://nbn-resolving.org/urn:nbn:de:101:1-2022122406314756438817
URL:
https://d-nb.info/1276509227/34
URL:
https://doi.org/10.1002/cbic.201500020
Bookmarklink