Format:
1 Online-Ressource
Content:
Abstract: L7Ae is a universal archaeal protein that recognizes and stabilizes kink-turn (k-turn) motifs in RNA substrates. These structural motifs are widespread in na-ture and are found in many functional RNA species, including ribosomal RNAs. Syn-thetic biology approaches utilize L7Ae/k-turn interactions to control gene expressionin eukaryotes. Here, we present results of comprehensive RNA immunoprecipitationsequencing (RIP-Seq) analysis of genomically tagged L7Ae from the hyperthermo-philic archaeonSulfolobus acidocaldarius. A large set of interacting noncoding RNAswas identified. In addition, several mRNAs, including thel7aetranscript, were foundto contain k-turn motifs that facilitate L7Ae binding.In vivostudies showed thatL7Ae autoregulates the translation of its mRNA by binding to a k-turn motif presentin the 5=untranslated region (UTR). A green fluorescent protein (GFP) reporter sys-tem was established inEscherichia coliand verified conservation of L7Ae-mediatedfeedback regulation inArchaea. Mobility shift assays confirmed binding to a k-turnin the transcript ofnop5-fibrillarin, suggesting that the expression of all C/D boxsRNP core proteins is regulated by L7Ae. These studies revealed that L7Ae-mediatedgene regulation evolved in archaeal organisms, generating new tools for the modu-lation of synthetic gene circuits in bacteria
Note:
mBio. - 8, 4 (2017) , e00730-17, ISSN: 2150-7511
Language:
English
DOI:
10.1128/mbio.00730-17
URN:
urn:nbn:de:bsz:25-freidok-1438360
URL:
https://doi.org/10.1128/mbio.00730-17
URL:
https://nbn-resolving.org/urn:nbn:de:bsz:25-freidok-1438360
Bookmarklink