Kooperativer Bibliotheksverbund

UID:

Format: xi, 262 Seiten , Illustrationen , 25.4 cm x 17.8 cm

ISBN: 1493972642 , 9781493972647

Series Statement: Methods in molecular biology 1663

Note: Includes bibliographical references and index , Super-resolution microscopy techniques and their potential for applications in radiation biophysics , Managing the introduction of super-resolution microscopy into a core facility , Live-cell STED imaging with the HyPer2 biosensor , Diffraction-unlimited fluorescence imaging with an EasySTED retrofitted confocal microscope , Two-photon STED microscopy for nanoscale imaging of neural morphology in vivo , STED imaging of golgi dynamics with Cer-SiR : a two-component, photostable, high-density lipid probe for live cells , Four-channel super-resolution imaging by 3-D structured illumination , Correlative SIM-STORM microscopy , Correlative super-resolution fluorescence imaging and atomic force microscopy for the characterization of biological samples , Quantitative single-molecule localization microscopy (qSMLM) of membrane proteins based on kinetic analysis of fluorophore blinking cycles , Two-color single-molecule tracking in live cells , Fully automated targeted confocal and single-molecule localization microscopy , Brain slice staining and preparation for three-dimensional super-resolution microscopy , Correlative in-resin super-resolution fluorescence and electron microscopy of cultured cells , Synthesis of Janelia Fluor HaloTag and SNAP-Tag ligands and their use in cellular imaging experiments , Measuring nanometer distances between fluorescent labels step-by-step , Correlative single-molecule localization microscopy and confocal microscopy , Correlative fluorescence super-resolution localization microscopy and platinum replica EM on unroofed cells , In situ super-resolution imaging of genomic DNA with oligoSTORM and oligoDNA-PAINT , Super-resolution high content screening and analysis

Additional Edition: Erscheint auch als Online-Ausgabe Super-resolution microscopy New York, NY : Humana Press, 2017 9781493972654

Language: English

Keywords: Laboratory Manuals

URL: Inhaltstext

URL: Cover

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Format: Online-Ressource (117 S.)

Note: Heidelberg, Univ., Diss., 2002

Additional Edition: Druckausg. Erfle, Holger Untersuchung von Membran-DNA Komplexen in äußeren elektrischen Feldern 2002

Language: German

Keywords: Hochschulschrift

UID:

Format: 1 Online-Ressource (XI, 262 p. 48 illus., 37 illus. in color)

ISBN: 9781493972654

Series Statement: Methods in Molecular Biology 1663

Content: This volume presents current advanced technologies and methods used in super-resolution microscopy. The chapters in this book cover a wide range of topics such as introducing super-resolution microscopy into a core facility; two-photon STED microscopy for nanoscale imaging of neural morphology in vivo; correlative SIM-STORM microscopy; two-color single-molecule tracking in live cells; and correlative single molecule localization microscopy and confocal microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Super-Resolution Microscopy: Methods and Protocols is a valuable resource for both established and novel researchers and users in this field

Additional Edition: Erscheint auch als Druck-Ausgabe ISBN 9781493972647

Language: English

DOI: 10.1007/978-1-4939-7265-4

URL: Volltext  (URL des Erstveröffentlichers)

UID:

Format: Online-Ressource (XI, 262 p. 48 illus., 37 illus. in color, online resource)

ISBN: 9781493972654

Series Statement: Methods in Molecular Biology 1663

Content: Super-Resolution Microscopy Techniques and their Potential for Applications in Radiation Biophysics -- Managing the Introduction of Super-Resolution Microscopy into a Core Facility -- Live-Cell STED Imaging with the HyPer2 Biosensor -- Diffraction-Unlimited Fluorescence Imaging with an EasySTED Retrofitted Confocal Microscope -- Two-Photon STED Microscopy for Nanoscale Imaging of Neural Morphology In Vivo -- STED Imaging of Golgi Dynamics with Cer-SiR: A Two-Component, Photostable, High-Density Lipid Probe for Live Cells -- Four Channel Super-Resolution Imaging by 3-D Structured Illumination -- Correlative SIM-STORM Microscopy -- Correlative Super-Resolution Fluorescence Imaging and Atomic Force Microscopy for the Characterization of Biological Samples -- Quantitative Single-Molecule Localization Microscopy (qSMLM) of Membrane Proteins Based on Kinetic Analysis of Fluorophore Blinking Cycles -- Two-Color Single-Molecule Tracking in Live Cells -- Fully Automated Targeted Confocal and Single Molecule Localization Microscopy -- Brain Slice Staining and Preparation for Three-Dimensional Super-Resolution Microscopy -- Correlative In-Resin Super-Resolution Fluorescence and Electron Microscopy of Cultured Cells -- Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and their use in Cellular Imaging Experiments -- Measuring Nanometer Distances between Fluorescent Labels Step-by-Step -- Correlative Single Molecule Localization Microscopy and Confocal Microscopy -- Correlative Fluorescence Super-Resolution Localization Microscopy and Platinum Replica EM on Unroofed Cells -- In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT -- Super-Resolution High Content Screening and Analysis.

Content: This volume presents current advanced technologies and methods used in super-resolution microscopy. The chapters in this book cover a wide range of topics such as introducing super-resolution microscopy into a core facility; two-photon STED microscopy for nanoscale imaging of neural morphology in vivo; correlative SIM-STORM microscopy; two-color single-molecule tracking in live cells; and correlative single molecule localization microscopy and confocal microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Super-Resolution Microscopy: Methods and Protocols is a valuable resource for both established and novel researchers and users in this field.

Additional Edition: 9781493972647

Additional Edition: Erscheint auch als Druck-Ausgabe Super-resolution microscopy New York, NY : Humana Press, 2017 1493972642

Additional Edition: 9781493972647

Language: English

DOI: 10.1007/978-1-4939-7265-4

URL: lizenzpflichtig

URL: Cover

UID:

Format: 117 S. , graph. Darst.

Note: Heidelberg, Univ., Diss., 2002

Additional Edition: Online-Ausg. Erfle, Holger Untersuchung von Membran-DNA Komplexen in äußeren elektrischen Feldern 2002

Language: German

Keywords: Hochschulschrift

UID:

Note: Dateiformat: tgz, Dateien im PDF-Format , Heidelberg, Univ., Diss., 2002

Additional Edition: Druckausg. Erfle, Holger Untersuchung von Membran-DNA-Komplexen in äußeren elektrischen Feldern

Language: German

Keywords: Hochschulschrift

URN: urn:nbn:de:bsz:16-opus-19359

URL: https://nbn-resolving.org/urn:nbn:de:bsz:16-opus-19359

URL: https://d-nb.info/963936050/34

URL: http://archiv.ub.uni-heidelberg.de/volltextserver/volltexte/2002/1935/container.tgz

URL: http://archiv.ub.uni-heidelberg.de/volltextserver/volltexte/2002/1935/index.html

UID:

Format: 117 Bl. , Ill., graph. Darst. , 30 cm

Note: Heidelberg, Univ., Diss., 2002 (Nicht für den Austausch)

Additional Edition: Online-Ausg. Erfle, Holger Untersuchung von Membran-DNA-Komplexen in äußeren elektrischen Feldern

Language: German

Keywords: Hochschulschrift

UID:

Format: Online-Ressource

Note: Dateiformat: tgz, Dateien im PDF-Format , Heidelberg, Univ., Diss., 2002

Language: German

URN: urn:nbn:de:bsz:16-opus-19359

URL: kostenfrei

URL: kostenfrei

URL: kostenfrei

UID:

Format: 117 Bl , Ill., graph. Darst

Note: Heidelberg, Univ., Diss., 2002

Language: German

Keywords: Hochschulschrift

UID:

Format: 1 Online-Ressource (8 Seiten, 190,11 KB)

Note: Projektleiter ist laut Berichtsblatt Autor , Durchführende Institution ist laut Berichtsblatt: BioQuant, Ruprecht-Karls-Universität Heidelberg , Unterschiede zwischen dem gedruckten Dokument und der elektronischen Ressource können nicht ausgeschlossen werden

Language: German

Keywords: Forschungsbericht

DOI: 10.2314/GBV:1022353632

URL: kostenfrei