Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
Type of Material
Type of Publication
Consortium
Language
  • 1
    Online Resource
    Online Resource
    Zürich : ETH
    UID:
    (DE-627)780840127
    Format: Online-Ressource (82 S) , Ill
    Edition: Online-Ausg.
    Note: Zugl.: Zürich, Diss., Eidgenössische Technische Hochschule ETH Zürich, Nr. 19539, 2011
    Language: English
    Keywords: Hochschulschrift
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    Microfilm
    Microfilm
    UID:
    (DE-627)689680546
    Format: 82 S. , Ill., graph. Darst.
    Edition: Mikrofiche-Ausg. 1 Mikrofiche
    Note: Zürich, Eidgen. Techn. Hochschule, Diss., 2011
    Language: English
    Keywords: Hochschulschrift
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    UID:
    (DE-627)1755742096
    Format: 4
    ISSN: 1545-7885
    Content: Apicomplexan parasites are defined by complex apical structures, which are necessary for interaction with incredibly diverse host cells. Two studies now amend a long-standing paradigm by showing conservation of an essential ring structure in the entire phylum.
    Note: Gesehen am 06.08.2021
    In: Public Library of Science, PLoS biology, Lawrence, KS : PLoS, 2003, 19(2021), 3, Artikel-ID e3001105, Seite 1-4, 1545-7885
    In: volume:19
    In: year:2021
    In: number:3
    In: elocationid:e3001105
    In: pages:1-4
    In: extent:4
    Language: English
    URL: Volltext  (lizenzpflichtig)
    URL: Volltext  (lizenzpflichtig)
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 4
    UID:
    (DE-627)1759074284
    Format: 8
    ISSN: 2235-2988
    Content: Regulating the number of progeny generated by replicative cell cycles is critical for any organism to best adapt to its environment. Classically, the decision whether to divide further is made after cell division is completed by cytokinesis and can be triggered by intrinsic or extrinsic factors. Contrarily, cell cycles of some species, such as the malaria-causing parasites, go through multinucleated cell stages. Hence, their number of progeny is determined prior to the completion of cell division. This should fundamentally affect how the process is regulated and raises questions about advantages and challenges of multinucleation in eukaryotes. Throughout their life cycle Plasmodium spp. parasites undergo four phases of extensive proliferation, which differ over three orders of magnitude in the amount of daughter cells that are produced by a single progenitor. Even during the asexual blood stage proliferation parasites can produce very variable numbers of progeny within one replicative cycle. Here, we review the few factors that have been shown to affect those numbers. We further provide a comparative quantification of merozoite numbers in several P. knowlesi and P. falciparum parasite strains, and we discuss the general processes that may regulate progeny number in the context of host-parasite interactions. Finally, we provide a perspective of the critical knowledge gaps hindering our understanding of the molecular mechanisms underlying this exciting and atypical mode of parasite multiplication.
    Note: Gesehen am 27.05.2021
    In: Frontiers in Cellular and Infection Microbiology, Lausanne : Frontiers Media, 2010, 11(2021) vom: 7. Mai, Artikel-ID 658616, Seite 1-8, 2235-2988
    In: volume:11
    In: year:2021
    In: day:7
    In: month:05
    In: elocationid:658616
    In: pages:1-8
    In: extent:8
    Language: English
    URL: Volltext  (kostenfrei)
    URL: Volltext  (kostenfrei)
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 5
    UID:
    (DE-627)1666008311
    Format: 6
    ISSN: 1872-9428
    Content: Immunofluorescence staining is the key technique for visualizing organization of endogenous cellular structures in single cells. Labeling and imaging of blood stage Plasmodium falciparum has always been challenging since it is a small intracellular parasite. A widely-used standard for parasite immunofluorescence is fixation in suspension with addition of minute amounts of glutaraldehyde to the paraformaldehyde-based solution. While this maintains red blood cell integrity, it has been postulated that antigenicity of the parasite proteins was, if at all, only slightly reduced. Here we show the deleterious effect that even these small quantities of glutaraldehyde can have on immunofluorescence staining quality and present an alternative cell seeding protocol that allows fixation with only paraformaldehyde. The highly improved signal intensity and staining efficiency enabled us to carry out RescueSTED nanoscopy on microtubules and nuclear pores and describe their organization in greater detail throughout the blood stage cycle.
    Note: Gesehen am 21.05.2019
    In: Molecular and biochemical parasitology, Amsterdam : Elsevier, 1980, 229(2019), Seite 47-52, 1872-9428
    In: volume:229
    In: year:2019
    In: pages:47-52
    In: extent:6
    Language: English
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 6
    UID:
    (DE-627)1841250651
    Format: 21 , Illustrationen
    ISSN: 1553-7374
    Content: Malaria remains a significant threat to global health, and despite concerted efforts to curb the disease, malaria-related morbidity and mortality increased in recent years. Malaria is caused by unicellular eukaryotes of the genus Plasmodium, and all clinical manifestations occur during asexual proliferation of the parasite inside host erythrocytes. In the blood stage, Plasmodium proliferates through an unusual cell cycle mode called schizogony. Contrary to most studied eukaryotes, which divide by binary fission, the parasite undergoes several rounds of DNA replication and nuclear division that are not directly followed by cytokinesis, resulting in multinucleated cells. Moreover, despite sharing a common cytoplasm, these nuclei multiply asynchronously. Schizogony challenges our current models of cell cycle regulation and, at the same time, offers targets for therapeutic interventions. Over the recent years, the adaptation of advanced molecular and cell biological techniques have given us deeper insight how DNA replication, nuclear division, and cytokinesis are coordinated. Here, we review our current understanding of the chronological events that characterize the unusual cell division cycle of P. falciparum in the clinically relevant blood stage of infection.
    Note: Gesehen am 05.12.2023
    In: Public Library of Science, PLoS pathogens, Lawrence, Kan. : PLoS, 2005, 19(2023), 3, Artikel-ID e1011157, Seite 1-21, 1553-7374
    In: volume:19
    In: year:2023
    In: number:3
    In: elocationid:e1011157
    In: pages:1-21
    In: extent:21
    Language: English
    URL: Volltext  (kostenfrei)
    URL: Volltext  (kostenfrei)
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 7
    UID:
    (DE-627)1683639278
    Format: 5
    ISSN: 1476-4687
    Content: Long-read sequencing allows the assembly of antigen-gene arrays in Trypanosoma brucei and, coupled with deletion experiments, demonstrates that histone variants act as a molecular link between genome architecture, chromatin conformation and antigen variation.
    Note: Gesehen am 27.11.2019 , Laura S.M. Müller and Raúl O. Cosentino contributed equally
    In: Nature 〈London〉, London [u.a.] : Nature Publ. Group, 1869, 563(2018), 7729, Seite 121-125, 1476-4687
    In: volume:563
    In: year:2018
    In: number:7729
    In: pages:121-125
    In: extent:5
    Language: English
    URL: Volltext  (Pay-per-use)
    URL: Volltext  (Pay-per-use)
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 8
    UID:
    (DE-627)1696938589
    Format: 11
    ISSN: 2150-7511
    Content: The human malaria parasite Plasmodium falciparum uses mutually exclusive expression of the PfEMP1-encoding var gene family to evade the host immune system. Despite progress in the molecular understanding of the default silencing mechanism, the activation mechanism of the uniquely expressed var member remains elusive. A GC-rich noncoding RNA (ncRNA) gene family has coevolved with Plasmodium species that express var genes. Here, we show that this ncRNA family is transcribed in a clonally variant manner, with predominant transcription of a single member occurring when the ncRNA is located adjacent to and upstream of an active var gene. We developed a specific CRISPR interference (CRISPRi) strategy that allowed for the transcriptional repression of all GC-rich members. A lack of GC-rich ncRNA transcription led to the downregulation of the entire var gene family in ring-stage parasites. Strikingly, in mature blood-stage parasites, the GC-rich ncRNA CRISPRi affected the transcription patterns of other clonally variant gene families, including the downregulation of all Pfmc-2TM members. We provide evidence for the key role of GC-rich ncRNA transcription in var gene activation and discovered a molecular link between the transcriptional control of various clonally variant multigene families involved in parasite virulence. This work opens new avenues for elucidating the molecular processes that control immune evasion and pathogenesis in P. falciparum. - IMPORTANCE Plasmodium falciparum is the deadliest malaria parasite species, accounting for the vast majority of disease cases and deaths. The virulence of this parasite is reliant upon the mutually exclusive expression of cytoadherence proteins encoded by the 60-member var gene family. Antigenic variation of this multigene family serves as an immune evasion mechanism, ultimately leading to chronic infection and pathogenesis. Understanding the regulation mechanism of antigenic variation is key to developing new therapeutic and control strategies. Our study uncovers a novel layer in the epigenetic regulation of transcription of this family of virulence genes by means of a multigene-targeting CRISPR interference approach.
    Note: Gesehen am 30.04.2020
    In: mBio, Washington, DC : American Society for Microbiology, 2010, 11(2020,1) Artikel-Nummer e03054-19, 11 Seiten, 2150-7511
    In: volume:11
    In: year:2020
    In: number:1
    In: extent:11
    Language: English
    URL: Volltext  (lizenzpflichtig)
    URL: Volltext  (lizenzpflichtig)
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 9
    UID:
    (DE-627)1878621807
    Format: 25 , Illustrationen
    ISSN: 1553-7374
    Content: Malaria is caused by the rapid proliferation of Plasmodium parasites in patients and disease severity correlates with the number of infected red blood cells in circulation. Parasite multiplication within red blood cells is called schizogony and occurs through an atypical multinucleated cell division mode. The mechanisms regulating the number of daughter cells produced by a single progenitor are poorly understood. We investigated underlying regulatory principles by quantifying nuclear multiplication dynamics in Plasmodium falciparum and knowlesi using super-resolution time-lapse microscopy. This confirmed that the number of daughter cells was consistent with a model in which a counter mechanism regulates multiplication yet incompatible with a timer mechanism. P. falciparum cell volume at the start of nuclear division correlated with the final number of daughter cells. As schizogony progressed, the nucleocytoplasmic volume ratio, which has been found to be constant in all eukaryotes characterized so far, increased significantly, possibly to accommodate the exponentially multiplying nuclei. Depleting nutrients by dilution of culture medium caused parasites to produce fewer merozoites and reduced proliferation but did not affect cell volume or total nuclear volume at the end of schizogony. Our findings suggest that the counter mechanism implicated in malaria parasite proliferation integrates extracellular resource status to modify progeny number during blood stage infection.
    Note: Gesehen am 22.01.2024
    In: Public Library of Science, PLoS pathogens, Lawrence, Kan. : PLoS, 2005, 19(2023), 12, Artikel-ID e1011807, Seite 1-25, 1553-7374
    In: volume:19
    In: year:2023
    In: number:12
    In: elocationid:e1011807
    In: pages:1-25
    In: extent:25
    Language: English
    URL: Volltext  (kostenfrei)
    URL: Volltext  (kostenfrei)
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 10
    UID:
    (DE-627)1777289017
    Format: 15
    ISSN: 2575-1077
    Content: 〈p〉Proliferation of 〈i〉Plasmodium falciparum〈/i〉 in red blood cells is the cause of malaria and is underpinned by an unconventional cell division mode, called schizogony. Contrary to model organisms, 〈i〉P. falciparum〈/i〉 replicates by multiple rounds of nuclear divisions that are not interrupted by cytokinesis. Organization and dynamics of critical nuclear division factors remain poorly understood. Centriolar plaques, the centrosomes of 〈i〉P. falciparum〈/i〉, serve as microtubule organizing centers and have an acentriolar, amorphous structure. The small size of parasite nuclei has precluded detailed analysis of intranuclear microtubule organization by classical fluorescence microscopy. We apply recently developed super-resolution and time-lapse imaging protocols to describe microtubule reconfiguration during schizogony. Analysis of centrin, nuclear pore, and microtubule positioning reveals two distinct compartments of the centriolar plaque. Whereas centrin is extranuclear, we confirm by correlative light and electron tomography that microtubules are nucleated in a previously unknown and extended intranuclear compartment, which is devoid of chromatin but protein-dense. This study generates a working model for an unconventional centrosome and enables a better understanding about the diversity of eukaryotic cell division.〈/p〉
    Note: Gesehen am 11.11.2021
    In: Life science alliance, Heidelberg : EMBO Press, 2018, 4(2021), 11, Artikel-ID e202101199, Seite 1-15, 2575-1077
    In: volume:4
    In: year:2021
    In: number:11
    In: elocationid:e202101199
    In: pages:1-15
    In: extent:15
    Language: English
    URL: Volltext  (lizenzpflichtig)
    URL: Volltext  (lizenzpflichtig)
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. Further information can be found on the KOBV privacy pages