Format:
1 Online-Ressource (PDF-Datei: 205 Seiten, 24186 Kilobyte)
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Illustrationen (farbig), Diagramme (farbig)
Content:
Streptococcus pneumoniae, Two-component system, Regulation
Content:
Streptococcus pneumoniae colonizes asymptomatically the upper respiratory tract as a commensal, but has also a high virulence potential and can leave this ecological niche, thereby spreading to the lungs and blood. During this process, pneumococci must adapt to changing external environmental conditions and parameters such as nutrient availability, temperature, or oxygen levels. The transmission of these signals into the bacterial cell interior occurs via the process of signal transduction, which ultimately results in controlled differential gene expression. The most commonly strategy for signal transduction is the use of two-component regulatory systems (TCS), consisting of a membrane-bound histidine kinase as a sensor and a cytoplasmic response regulator that binds to the promoter region of its target genes and interferes with gene expression. In this study the regulatory impact and influence of the TCS08 and TCS09 on the phenotype and pathophysiology of S. pneumoniae were investigated using two different serotypes (serotype 2: D39 and serotype 4: TIGR4). For all functional assays, single (Δrr08/Δrr09 or Δhk08/Δhk09) and double (Δtcs08 or Δtcs09) mutants that were constructed by insertion-deletion mutagenesis, were applied. In the first study a comparative transcriptome analysis using RNA-sequencing was conducted with our tcs09-mutants and the parental wild-type D39. The data indicated upregulation of the aga operon, which is related to galactose metabolism, and ...
Note:
Literaturverzeichnis: Seite 165-188. - Literaturangaben
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Dissertation Mathematisch-Naturwissenschaftliche Fakultät der Universität Greifswald 2021
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Mit deutscher Zusammenfassung
Additional Edition:
Erscheint auch als Druck-Ausgabe Hirschmann, Stephanie Impacts of TCS08 and TCS09 on the pathophysiology of Streptococcus pneumoniae Greifswald, 2021
Language:
English
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German
Keywords:
Streptococcus pneumoniae
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Zweikomponentensystem
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Regulation
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Pathophysiologie
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Genexpression
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Hochschulschrift
URN:
urn:nbn:de:gbv:9-opus-56791
URL:
https://nbn-resolving.org/urn:nbn:de:gbv:9-opus-56791
URL:
https://d-nb.info/1243168897/34
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