Format:
1 online resource (359 pages)
Edition:
1st ed.
ISBN:
9783527608706
Content:
Dr Hrabe de Angelis is Director if theInstitute of Experimental Genetics, in Munich, Germany and Director of the European Mouse Mutant Archive (EMMA), in Monterotondo, Italy. He is also cofounder of "INGENIUM Biopharmaceuticals AG". Processor Pierre Chambon is the director of the Institute of Genetics, Molecular and Cell Biology in Strasbourg. He has won numerous scientific prizes including the CNRS Gold Medal and the Grand Prix of the Foundation of Medical Reseach. Professor Steve Brown is Director of the Mammalian Genetics Unit of the MRC in Harwell, UK, and is a member of EMBO.
Content:
Intro -- Standards of Mouse Model Phenotyping -- Foreword -- Table of Contents -- Preface -- 1 Characterizing Hearing in Mice -- 1.1 Introduction -- 1.2 Behavioral Tests of Hearing -- 1.3 Physiological Tests of Hearing -- 1.4 Anatomy of the Ear -- 1.5 Conclusions -- Acknowledgements -- 2 Molecular Phenotyping: Gene Expression Profiling -- 2.1 Why this Screen? Medical and Biological Relevance -- 2.2 Examples: Diseases of Mouse and Man -- 2.3 Diagnostic Methods: History and State of the Art -- 2.4 Technical Requirements for Screening Protocols (Short): First and Second Line Approaches -- 2.5 Logistics (Whom, When, How Many, Why) -- 2.5.1 Choice of Platform -- 2.5.2 Biological Samples -- 2.6 Trouble Shooting -- 2.6.1 Preparation of Hybridization Target -- 2.6.2 Critical Issues of Chip Hybridization -- 2.6.3 Image Processing and Array Design -- 2.7 Short-term Outlook -- 3 Screening for Bone and Cartilage Phenotypes in Mice -- 3.1 Introduction -- 3.1.1 The Skeleton -- 3.1.2 Skeletal Development in the Embryo -- 3.1.3 Growth and Maintenance of Bone and Cartilage -- 3.1.4 Diseases Involving Cartilage and Bone -- 3.1.5 The Mouse as a Model for Skeletal Diseases -- 3.2 Screening Protocols -- 3.2.1 Morphological Analysis -- 3.2.1.1 Protocol -- 3.2.2 X-Ray Analysis -- 3.2.2.1 General -- 3.2.2.2 Imaging -- 3.2.2.3 X-Ray Analysis -- 3.2.2.4 Protocol -- 3.2.3 DXA-Analysis -- 3.2.3.1 General -- 3.2.3.2 Advantages -- 3.2.3.3 Disadvantages -- 3.2.3.4 Small Animal Applications -- 3.2.3.5 Precision and Accuracy -- 3.2.3.6 Considerations -- 3.2.3.7 Protocol -- 3.2.4 Biochemical Bone Markers -- 3.2.4.1 Clinical Utility of Biochemical Markers of Bone Turnover in Small Animals -- 3.2.4.2 Mouse Markers of Bone Turnover/Metabolism and Hormonal Regulation -- 3.2.4.3 Variability/Sensitivity/Sample Choice -- 3.2.4.4 Which Markers Should be Used During the Screen?.
Note:
Description based on publisher supplied metadata and other sources
Additional Edition:
9783527310319
Additional Edition:
Erscheint auch als Druck-Ausgabe 9783527310319
Language:
English
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